A5055316622- Glaucoma and retinal disorders
- Adenosine and Purinergic Signaling
- Corneal surgery and disorders
- Cellular Mechanics and Interactions
- Retinal Diseases and Treatments
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Retinal and Macular Surgery
- Ophthalmology and Eye Disorders
- Photoacoustic and Ultrasonic Imaging
- Gastroesophageal reflux and treatments
- Pregnancy and Medication Impact
- Optical Coherence Tomography Applications
- Signaling Pathways in Disease
- Caveolin-1 and cellular processes
- Hippo pathway signaling and YAP/TAZ
- Reproductive System and Pregnancy
- Retinal Development and Disorders
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Skin and Cellular Biology Research
- Advanced Fluorescence Microscopy Techniques
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Leprosy Research and Treatment
Oregon Health & Science University
2006-2024
University of Wisconsin–Madison
2001-2007
University of Portland
2005
A real-time line-field optical coherence tomography (LF-OCT) system is demonstrated with image acquisition rates of up to 5000 B-frames or 2.5 million A-lines per second for 500 B-frame. The uses a high-speed low-cost camera achieve continuous data transfer required imaging, allowing the evaluation future applications in clinical intraoperative environments. light source an 840 nm super-luminescent diode. Leveraging parallel computing GPU and high speed CoaXPress interface, we were able...
Abstract Extracellular nucleotides regulate macrophage function via P2X nucleotide receptors that form ligand-gated ion channels. In particular, P2X7 activation is characterized by pore formation, membrane blebbing, and cytokine release. also linked to mitogen-activated protein kinases (MAPK) Rho-dependent pathways, which are known affect cytoskeletal structure in other systems. As critical for behavior, we have tested the importance of these pathways actin filament reorganization during...
A single nucleotide polymorphism (SNP) identified between caveolin-1 (CAV1) and caveolin-2 (CAV2) on chromosome 7 is associated with glaucoma. One function of CAVs endocytosis recycling extracellular matrix (ECM) components. Here, we generated CAV-silencing lentivirus to evaluate the effects ECM turnover by trabecular meshwork (TM) cells measure effect outflow facility in anterior segment perfusion culture.Short hairpin CAV1 CAV2 silencing control were generated, characterized, applied...
Extracellular nucleotides such as ATP are present in abundance at sites of inflammation and tissue damage, these agents exert a potent modulatory effect on macrophage/monocyte function via the nucleotide receptor P2X(7). In this regard, after exposure to bacterial LPS, P2X(7) activation augments expression inducible nitric oxide (NO) synthase production NO macrophages. Because has been reported stimulate certain members MAP kinase family (ERK1/2) can enhance DNA-binding activity NF-kappa B,...
There are distinctive areas of colocalization matrix metalloproteinase (MMP)-2 and -14 on trabecular meshwork (TM) cells that resemble podosomes or invadopodia. Studies were conducted to determine whether TM exhibit podosome- invadopodia-like structures (PILS) they produce focal extracellular (ECM) turnover.Porcine human perfused anterior segment organ cultures studied. Localization PILS components in sections from segments was determined by immunohistochemistry confocal microscopy. Cells...
Previous reports about the nucleotide receptor P2X(7), which exhibits ion channel and pore-forming activity is known to promote IL-1beta processing, have centered largely on its role in macrophage function, whereas participation monocyte has been unclear. However, because extracellular ATP shown affect monocytes with respect release, we hypothesized that P2X(7) also present functional a subpopulation of blood monocytes. Flow cytometric analysis revealed 70% isolated from normal human donors...
Macrophages express several lipopolysaccharide (LPS) binding proteins and are potently activated by LPS to produce inflammatory mediators. Recent studies have shown that receptors for exogenous nucleotides (P2X P2Y purinergic receptors) can modulate macrophage production of TNF-α , IL-1β nitric oxide (NO) following exposure. LPS-stimulated monocytes elevated levels 1 2 P2X 7 mRNA, suggesting both contribute LPS-induced pathophysiology. In addition, oxidized-ATP treatment (which inhibits )...
Macrophages express several lipopolysaccharide (LPS) binding proteins and are potently activated by LPS to produce inflammatory mediators. Recent studies have shown that receptors for exogenous nucleotides (P2X P2Y purinergic receptors) can modulate macrophage production of TNF-alpha, IL-1beta nitric oxide (NO) following exposure. LPS-stimulated monocytes elevated levels P2Y1, P2Y2 P2X7 mRNA, suggesting both P2X contribute LPS-induced pathophysiology. In addition, oxidized-ATP treatment...
Abstract During infection or inflammation, high concentrations of extracellular nucleotides are released into the inflammatory microenvironment, supplying a source ligand for purinergic receptors that present on many immune cell types. The P2X 7 receptor, member receptor family ATP‐gated ion channels, is thought to play an important role in monocyte/macrophage activation. One factor can powerfully activate macrophages bacterial lipopolysaccharide (endotoxin, LPS) and although mechanisms...
Intraocular pressure (IOP) elevation is the primary risk factor and currently main treatable for progression of glaucomatous optic neuropathy. In addition to direct clinical living animal in vivo studies, ex perfusion anterior segments whole eyes a key technique studying conventional outflow function as it responsible IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, limitations several model systems These include: (1) perfused globes, (2)...
Corneal disease is the fifth leading cause of global blindless. Optical coherence tomography (OCT) for anterior imaging extensively used due to its non-invasive and high-resolution volumetric characteristics. microscopy (OCM) a technical variation OCT that can image cornea with cellular resolution. Here, we demonstrate visible-light OCM as low-cost easily reproducible system visualize various corneal structures such epithelial cells, endothelial keratocytes, collagen bundles within stromal...