A5027515175- Glaucoma and retinal disorders
- Hippo pathway signaling and YAP/TAZ
- Proteoglycans and glycosaminoglycans research
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Corneal surgery and disorders
- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Bone fractures and treatments
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- TGF-β signaling in diseases
- Knee injuries and reconstruction techniques
- Adipose Tissue and Metabolism
- Bone Metabolism and Diseases
- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Ocular Surface and Contact Lens
- Muscle Physiology and Disorders
- Autophagy in Disease and Therapy
- Connexins and lens biology
- Ophthalmology and Eye Disorders
- Cancer-related molecular mechanisms research
- S100 Proteins and Annexins
SUNY Upstate Medical University
2017-2025
Syracuse University
2020-2024
Case Western Reserve University
2014-2021
Forest Institute
2017-2019
Wake Forest University
2017-2019
Charlie Norwood VA Medical Center
2012-2014
Georgia Regents Medical Center
2013-2014
Augusta University
2010-2014
Augusta University Health
2012-2013
University of Georgia
2012
Mechanical loading regulates mesenchymal condensation–mediated endochondral bone regeneration.
Remote ischemic conditioning is cardioprotective in myocardial infarction and neuroprotective mechanical occlusion models of stroke. However, there no report on its therapeutic potential a physiologically relevant embolic stroke model (embolic middle cerebral artery occlusion) combination with intravenous tissue-type plasminogen activator (tPA).We tested remote perconditioning therapy (RIPerC) at 2 hours after the mouse without tPA 4 hours. We assessed blood flow up to 6 hours, neurological...
Primary open-angle glaucoma progression is associated with increased human trabecular meshwork (HTM) stiffness and elevated transforming growth factor beta 2 (TGFβ2) levels in the aqueous humor. Increased transcriptional activity of Yes-associated protein (YAP) coactivator PDZ-binding motif (TAZ), central players mechanotransduction, are implicated glaucomatous HTM cell dysfunction. Yet, detailed mechanisms underlying YAP/TAZ modulation cells response to alterations extracellular matrix...
Abstract Bone tissue engineering via endochondral ossification has been explored by chondrogenically priming cells using soluble mediators for at least 3 weeks to produce a hypertrophic cartilage template. Although recapitulation of achieved, long-term in vitro culture is required through repeated supplementation inductive factors the media. To address this challenge, microparticle-based growth factor delivery system was engineered drive within human bone marrow-derived mesenchymal stem cell...
Human hematopoietic niches are complex specialized microenvironments that maintain and regulate stem progenitor cells (HSPC). Thus far, most of the studies performed investigating alterations HSPC-niche dynamic interactions conducted in animal models. Herein, organ microengineering with microfluidics is combined to develop a human bone marrow (BM)-on-a-chip an integrated recirculating perfusion system consolidates variety important parameters such as 3D architecture, cell-cell/cell-matrix...
Lung cancer is the leading cause of cancer-related death worldwide yet in vitro disease models have been limited to traditional 2D culture utilizing cell lines. In contrast, recently developed 3D (organoids) adopted by researchers improve physiological relevance laboratory study. We hypothesized that hydrogel-based will allow for improved replication and characterization over standard using cells taken directly from patients. Here, we leveraged use create lung organoids a unique source,...
Mesenchymal condensations promote defect repair by mimicking cellular, morphogenetic, and mechanical aspects of bone development.
Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) hold great potential for cell-based therapy, yet the therapeutic efficacy remains uncertain. Transplanted BMSCs often fail to engraft within bone marrow (BM), in part due poor survival of donor response inflammatory reactions, hypoxia, oxidative stress, or nutrient starvation. Two basic cell processes, apoptosis and autophagy, could potentially be responsible impaired transplanted BMSCs. However, functional relationship between...
Increasing evidence suggests that stromal cell-derived factor-1 (SDF-1/CXCL12) is involved in bone formation, though underlying molecular mechanisms remain to be fully elucidated. Also, contributions of SDF-1β, the second most abundant splice variant, as an osteogenic mediator obscure. We have shown SDF-1β enhances osteogenesis by regulating morphogenetic protein-2 (BMP-2) signaling vitro. Here we investigate dose-dependent contribution suboptimal BMP-2-induced local formation; is, a dose...
Abstract The growing socioeconomic burden of musculoskeletal injuries and limitations current therapies have motivated tissue engineering approaches to generate functional tissues aid in defect healing. A readily implantable scaffold-free system comprised human bone marrow-derived mesenchymal stem cells embedded with bioactive microparticles capable controlled delivery transforming growth factor-beta 1 (TGF-β1) morphogenetic protein-2 (BMP-2) was engineered guide endochondral formation. were...
Abstract The tumor microenvironment (TME) plays a significant role in cancer progression and thus modeling it will advance our understanding of growth dynamics response to therapies. Most vitro models are not exposed intact body physiology, at the same time, fail recapitulate extensive features stroma. Conversely, animal do accurately capture human architecture. We address these deficiencies with biofabricated colorectal (CRC) tissue equivalents, which built replicate architectural biopsied...
Impairment of the trabecular meshwork (TM) is principal cause increased outflow resistance in glaucomatous eye. Yes-associated protein (YAP) and transcriptional coactivator with PDZ binding motif (TAZ) are emerging as potential mediators TM cell/tissue dysfunction. Furthermore, YAP/TAZ activity was recently found to be controlled by mevalonate pathway non-ocular cells. Clinically used statins block cascade were shown improve cell pathobiology; yet, link signaling not investigated. In this...
The risk for developing primary open-angle glaucoma (POAG) correlates with the magnitude of ocular hypertension (OHT) and concentration transforming growth factor-β2 (TGFβ2) in aqueous humor. Effective treatment POAG requires detailed understanding interaction between pressure sensing mechanisms trabecular meshwork (TM) biochemical factors. Here, we employed molecular, optical, electrophysiological tonometric strategies to establish role TGFβ2 transcription functional expression...
The risk for developing primary open-angle glaucoma (POAG) correlates with the magnitude of ocular hypertension (OHT) and concentration transforming growth factor-β2 (TGFβ2) in aqueous humor. Effective treatment POAG requires detailed understanding interaction between pressure sensing mechanisms trabecular meshwork (TM) biochemical factors. Here, we employed molecular, optical, electrophysiological tonometric strategies to establish role TGFβ2 transcription functional expression...
Skeletal injuries are among the most prevalent clinical problems and bone marrow-derived mesenchymal stem/stromal cells (BMSCs) have successfully been used for treatment thereof. Stromal cell-derived factor-1 (SDF-1; CXCL12) is a member of CXC chemokine family with multiple splice variants. The two abundant variants, SDF-1α SDF-1β, share identical amino acid sequences, except four additional acids at C-terminus which may mediate surface stabilization via glycosaminoglycans protect SDF-1β...
Abstract Currently, there are no synthetic or biologic materials suitable for long‐term treatment of large tracheal defects. A successful replacement must (1) have radial rigidity to prevent airway collapse during respiration, (2) contain an immunoprotective respiratory epithelium, and (3) integrate with the host vasculature support epithelium viability. Herein, biopolymer microspheres used deliver chondrogenic growth factors human mesenchymal stem cells (hMSCs) seeded in a custom mold that...
Kwon DH, Bennett W, Herberg S, Bastone P, Pippig Rodriguez NA, Susin C, Wikesjö UME. Evaluation of an injectable rhGDF‐5/PLGA construct for minimally invasive periodontal regenerative procedures: a histological study in the dog. J Clin Periodontol 2010; 37: 390–397. doi: 10.1111/j.1600‐051X.2010.01546.x. Abstract Aim: To evaluate injectability, biocompatibility, safety, and wound healing/regeneration following application novel bioresorbable recombinant human growth/differentiation factor‐5...
Purpose Transforming growth factor-beta 2 (TGFβ2) is a major contributor to the pathologic changes occurring in human trabecular meshwork (HTM) cells primary open-angle glaucoma (POAG). TGFβ2 activates extracellular-signal-regulated kinase (ERK) and Rho-associated (ROCK) signaling pathways, both affecting HTM cell behavior. However, exactly how these pathways converge regulate contractility unclear. Here, we investigated molecular mechanism underlying TGFβ2-induced contractility, crosstalk...