A5039430712- Osteoarthritis Treatment and Mechanisms
- Silk-based biomaterials and applications
- Bone Tissue Engineering Materials
- Alkaline Phosphatase Research Studies
- Bone and Dental Protein Studies
- Nasal Surgery and Airway Studies
- Collagen: Extraction and Characterization
- Reconstructive Facial Surgery Techniques
- Bone Metabolism and Diseases
- Advanced Glycation End Products research
- Ear Surgery and Otitis Media
- Planarian Biology and Electrostimulation
- Olfactory and Sensory Function Studies
- Marine Biology and Environmental Chemistry
- Periodontal Regeneration and Treatments
- Protein Hydrolysis and Bioactive Peptides
- Bone health and treatments
- Cytokine Signaling Pathways and Interactions
- Diabetic Foot Ulcer Assessment and Management
Hospital for Special Surgery
2008-2010
Cornell University
2008-2009
University of Massachusetts Chan Medical School
2002-2004
Worcester Polytechnic Institute
2001
Columbia University
2000
This study focuses on the development of a novel method nonenzymatic glycation fibrillar collagen gels. In contrast to previous studies in which type I gels were glycated solid state, this presents for solution. The solution or was exposed range ribose concentrations from 0 250 mM. binding documented using Fourier transform infrared (FTIR) spectroscopy. formation advanced end products (AGEs) quantified by fluorescence measurement. bulk compressive modulus and viscoelastic time constant...
Using a custom galvanotaxis chamber and time-lapse digital video microscopy, we report the novel observation that cultured chondrocytes exhibit cathodal migration when subjected to applied direct current (DC) electric fields as low 0.8 V/cm. The response was dose-dependent for field strengths greater than 4 Cell appeared be an active process with extension of cytoplasmic processes in direction movement. In some cells, application hour induced elongation initially round cells accompanied by...
Abstract A large‐deflection elasticity model was used to describe the mechanical behavior of cartilaginous tissues during three‐point bending tests. Force‐deflection curves were measured for 20‐mm long × 4‐mm wide ≈1‐mm thick strips porcine auricular and costal cartilage. Using a least‐squares method with elastic modulus in as only adjustable parameter, data fit based on von Karman theory large deflection plates. This described well, an average RMS error 14.8% R 2 value 0.98. this method,...
Abstract Collagen glycated with ribose (250 mM) in solution (pre‐glycation) and as a gel (post‐glycation) was seeded chondrocytes the effects of glycation on chondrocyte matrix assembly culture were determined. Pre‐glycation enhanced GAG accumulation significantly over controls at both 2 4 weeks ( p < 0.05), although time points there no statistical differences cell number between pre‐glycated control gels. The increased proteoglycan shown to be part due retention by constructs 0.05)....
Abstract Chondrocyte apoptosis is thought to be an important step in the calcification of cartilage vivo; however, there are conflicting reports as whether or not this a necessary precursor mineralization. The goal study was determine for mineralization vitro murine micromass model endochondral ossification. C3H10T1/2 mesenchymal stem cells were plated culture presence 4 mM inorganic phosphate with addition apoptogens, camptothecin, staurosporine, induce apoptosis. rate and total...
Chick limb-bud mesenchymal stem cells plated in high density culture the presence of 4 mM inorganic phosphate and vitamin C differentiate form a mineralizable matrix, resembling that chick growth plate. To further elucidate mechanism allows these cultures to physiologic hydroxyapatite deposits, how process can be manipulated gain insight into mineralization mechanisms, we compared gene expression mineralizing (with phosphate) non-mineralizing (containing only 1 at start (day 11) after...
Abstract The current literature presents many techniques for analysis of the mechanical properties articular cartilage [1]. By comparison few are available auricular which experiences a different environment than cartilage. Specifically, large deflection bending is deformation mode that most relevant to cartilage, but has not been studied thoroughly in soft tissues. goals this study were to: 1) apply an elasticity model three point data from normal and engineered costal cartilage; 2) use...
A large deflection elasticity model was used to describe the tensile elastic modulus of cartilage tissues during three point bending tests. Native and engineered were compared for both auricular costal types.
Abstract Cultured chondrocytes exhibit cathodal migration when subjected to applied direct current (DC) electric fields [1]. Our studies showed a 2-fold enhancement in migratory velocity at physiologic temperature (∼37°C) compared room (∼25°C). The inositol phosphate pathway antagonists, U-73122 and neomycin, were found inhibit the directed response, indicating that it may be involved chondrocyte migration. Further understanding of molecular mechanisms underlying galvanotaxis contribute...