ObjectivesReduced susceptibility to extended-spectrum cephalosporins in Neisseria gonorrhoeae has, date, been associated with three alterations: a mosaic penA allele encoding the penicillin-binding protein 2 (PBP2); A-del-mtrR, an adenine deletion mtrR promoter; and penB, comprising mutated alleles of PorBIb. In this study, we examined association between reduced ceftriaxone additional mutations gonococcal PBP2.

<h3>Objectives</h3> To investigate the performance of fully automated cobas 4800 CT/NG test for detection <i>Chlamydia trachomatis</i> and <i>Neisseria gonorrhoeae</i>. <h3>Methods</h3> The study was conducted using 900 clinical specimens (496 urine 404 swab specimens) <i>C testing, which 498 (318 180 were also tested <i>N results compared with those obtained from Roche COBAS AMPLICOR TaqMan CT assays. <i>N</i> gonorrhoeae-positive further in-house, real-time PCR A panel 223 <i>Neisseria</i>...

ABSTRACT With increasing concerns regarding diminishing treatment options for gonorrhea, maintaining the efficacy of currently used treatments and ensuring optimal Neisseria gonorrhoeae antimicrobial resistance surveillance are utmost importance. Penicillin is still to treat gonorrhea in some parts world. In this study, we developed validated a real-time PCR assay detection penicillinase-producing N. (PPNG) noncultured clinical samples with aim enhancing penicillin surveillance. The...

The gonococcal porA pseudogene is a popular target for in-house Neisseria gonorrhoeae PCR methods. With this study we present two novel findings: the first case of an N. false-negative result caused by sequence variation, and in same organism, description clinical strain harbouring meningitidis sequence.

ObjectivesUnlike most of the world, penicillin resistance in Neisseria gonorrhoeae from remote regions Western Australia (WA) with high gonorrhoea notification rates has not increased despite many years empirical oral therapy. With advent non-culture molecular diagnosis and consequent decline culture-based susceptibility, it is imperative to ensure ongoing reliability combination azithromycin, amoxicillin probenecid for uncomplicated this setting. PCR-based N. antimicrobial surveillance...

Recent emergence of the extensively drug-resistant Neisseria gonorrhoeae H041 strain in Japan raises concerns that gonorrhoea may soon become untreatable and emphasizes need for enhanced surveillance. In this study we developed a real-time PCR assay direct detection strain.Two assays penA gene strain, H041-PCR1 H041-PCR2, were evaluated parallel. Assay performance was assessed using panel pathogenic commensal species (n = 167 strains) including N. clinical specimens 252) submitted sexual...

<h3>Objectives</h3> The <i>Neisseria gonorrhoeae</i> multi-antigen sequence typing (NG-MAST) system, based on PCR amplification and analysis of the gonococcal <i>porB</i> <i>tbpB</i> genes, is widely used for molecular isolates but not validated non-cultured clinical samples. This study sought to examine performance NG-MAST system a range <h3>Methods</h3> Nucleic acid extracts 73 <i>N gonorrhoeae</i>-positive samples, comprising eight cervical swabs, nine urethral 35 urine one vaginal swab,...

Neisseria gonorrhoeae has developed resistance to multiple classes of antimicrobials. There is now growing concern that without the availability appropriate public health strategies combat this problem, gonorrhea could become untreatable. For reason, surveillance for gonococcal antimicrobial must be optimal both in terms obtaining a representative sample isolates and having tools identify resistance. To aid with surveillance, molecular are increasingly being used. In present study, we...

Emergence and spread of Neisseria gonorrhoeae resistant to extended spectrum cephalosporins is a major problem threatening treatment gonorrhoea further highlighted by the recent report second ceftriaxone-resistant N. strain (F89) in Europe, initially observed France subsequently identified Spain. antimicrobial resistance (AMR) surveillance has acquired new importance molecular tools have potential enhance bacterial culture-based methods. In this study, we established polymerase chain...

Previous studies have shown that mixed-strain gonococcal infections can occur. However, it remains unclear whether such impact upon the reliability of Neisseria gonorrhoeae antimicrobial resistance (AMR) surveillance. In this study, we aimed to resolve question by intensively sampling isolates from gonorrhoea-positive specimens in a high-risk population Sydney, Australia. A total 615 N. isolates, originating 63 clinical samples (31 rectal swabs and 32 throat swabs), were characterized. All...

ABSTRACT A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay validated by testing 342 PPNG isolates and 415 clinical samples. method is suitable routine strains. Its approach reduces potential false-negative results caused sequence variations.

The remote and indigenous populations of Western Australia (WA) have one the highest notification rates gonorrhoea in world. Despite this, low rate antimicrobial resistance Neisseria gonorrhoeae from these regions permits use amoxycillin as empirical therapy. We describe first molecular epidemiological study gonococci isolated this population using two different typing platforms.Pulse-field gel electrophoresis (PFGE), multi-antigen sequence (NG-MAST) susceptibility tests were performed on...

ObjectivesNeisseria gonorrhoeae multilocus sequence typing (MLST) is a key tool used to investigate the macroepidemiology of gonococci exhibiting antimicrobial resistance (AMR). However, utility MLST undermined by high workload and cost associated with DNA sequencing seven housekeeping genes. In this study, we investigated single nucleotide polymorphism (SNP)-based profiling as means circumventing these problems.

With treatment options for gonorrhoea (Neisseria gonorrhoeae) diminishing, strengthening antimicrobial resistance (AMR) surveillance is paramount.In this study, we investigated polymerase chain reaction (PCR) based methods, in parallel with N. gonorrhoeae multi-antigen sequence typing (NG-MAST), direct detection of four chromosomal mechanisms associated emerging to extended spectrum cephalosporins using noncultured samples: an adenine deletion the mtrR promoter, a mosaic penicillin-binding...

Introduction Mycobacterium tuberculosis continues to have a high mortality and morbidity burden globally. IGRA such as Quantiferon Gold Plus aid in diagnosis of latent TB but pleural remains challenging. We present case active MTB infection with reversion from positive negative, negative Xpert MTB/RIF Ultra PCR results biopsy tissues. Case A 52-year-old male presented 2022 two-week history pleuritic chest pain associated modest elevation inflammatory markers. The patient had QFG-Plus result...

Introduction. Mycobacterium tuberculosis (MTB) infections continue to have a high mortality and morbidity burden globally. Interferon-gamma release assays such as Quantiferon Gold Plus (QFG-Plus) aid in diagnosis of latent TB but pleural remains challenging. We present case active MTB infection with reversion from positive negative IGRA result well Xpert MTB/RIF Ultra PCR tissues obtained biopsy. Case summary. A 52-year-old otherwise healthy male presented August 2022 2 week history...