A5019047669- Gut microbiota and health
- Pediatric health and respiratory diseases
- Asthma and respiratory diseases
- Prostate Cancer Treatment and Research
- Prostate Cancer Diagnosis and Treatment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Infant Health and Development
- Cytomegalovirus and herpesvirus research
- Inhalation and Respiratory Drug Delivery
- Molecular Biology Techniques and Applications
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- GDF15 and Related Biomarkers
- Heart Failure Treatment and Management
- Health Systems, Economic Evaluations, Quality of Life
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer-related molecular mechanisms research
- Breastfeeding Practices and Influences
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Respiratory and Cough-Related Research
- Transplantation: Methods and Outcomes
- Cardiovascular Function and Risk Factors
- HIV/AIDS oral health manifestations
- Clostridium difficile and Clostridium perfringens research
British Columbia Children's Hospital
2019-2025
University of British Columbia
2015-2025
Child and Family Research Institute
2025
Prevention of Organ Failure
2015-2019
Decipher Biosciences (Canada)
2019
BC Centre for Disease Control
2019
Genome British Columbia
2017-2018
Vancouver Biotech (Canada)
2016
St. Paul's Hospital
2016
Allergic diseases affect millions of people worldwide. An increase in their prevalence has been associated with alterations the gut microbiome, i.e., microorganisms and genes within gastrointestinal tract. Maturation infant immune system microbiota occur parallel; thus, conformation microbiome may determine if tolerant programming arises infant. Here we show, using deeply phenotyped participants CHILD birth cohort (n = 1115), that there are early-life influences features which uniformly four...
Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk. Breastfeeding acts as a primary modulator during early life, but its effect on development has remained unclear.We harnessed CHILD cohort interrogate influence breastfeeding antibiotic-associated risk in subset children (n = 2,521). We then profiled microbiomes these 1,338) using shotgun metagenomic sequencing compared human milk oligosaccharide fatty acid...
Graphical abstractAbstractBackgroundAtopic dermatitis (AD) is the most common chronic inflammatory skin disease in both pediatric and adult populations. The development of AD has been linked to antibiotic usage, which causes perturbation microbiome associated with abnormal immune system function. However, imbalances gut itself usage have inconsistently AD.ObjectivesThis study aimed elucidate timing specific factors mediating relationship between systemic (oral or intravenous) AD.MethodsWe...
Recent evidence suggests an immunomodulatory role for commensal fungi (mycobiota) in the gut, yet little is known about composition and dynamics of early-life gut fungal communities. In this work, we show first time that mycobiota Canadian infants changes dramatically over course year life, associated with environmental factors such as geographical location, diet, season birth, can be used conjunction knowledge a small number key to predict inhalant atopy status at age 5 years.
Abstract Background Early identification of children at risk asthma can have significant clinical implications for effective intervention and treatment. This study aims to disentangle the relative timing importance early markers asthma. Methods Using CHILD Cohort Study, 132 variables measured in 1754 multi-ethnic were included analysis prediction. Data up 4 years age was used multiple machine learning models predict physician-diagnosed 5 years. Both predictive performance variable assessed...
<h3>Background</h3> Despite the significant morbidity and mortality related to pulmonary exacerbations in cystic fibrosis (CF), there remains no reliable predictor of imminent exacerbation. <h3>Objective</h3> To identify blood-based biomarkers predict (<4 months from stable blood draw) CF using targeted proteomics. <h3>Methods</h3> 104 subjects provided plasma samples when clinically were randomly split into discovery (n=70) replication (n=34) cohorts. Multiple reaction monitoring mass...
Abstract Background/Objective The steep rise in childhood obesity has emerged as a worldwide public health problem. first 4 years of life are critical window where long-term developmental patterns body mass index (BMI) established and period for microbiota maturation. Understanding how the early-life relate to preschool growth may be useful identifying preventive interventions obesity. We aim investigate whether longitudinal shifts within bacterial community between 3 months 1 year...
Abstract BACKGROUND Cholesterol efflux capacity (CEC) is a measure of HDL function that, in cell-based studies, has demonstrated an inverse association with cardiovascular disease. The CEC complex and low-throughput. We hypothesized that assessment the lipoprotein proteome would allow for precise, high-throughput prediction. METHODS After isolating particles from serum, we used LC-MS/MS to quantify 21 lipoprotein-associated proteins. A bioinformatic pipeline was identify proteins univariate...
Objective Lower respiratory tract infections (LRTIs) in early life are one of the strongest risk factors for childhood asthma and often treated with systemic antibiotics (IV or oral). We aimed to explore association between early-life LRTIs on development potential mediating role this relationship. Methods Data were collected as part longitudinal, general Canadian population CHILD Study. during first 18 months identified through parental symptom report at regular study visits. Systemic...
Background Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) result in considerable morbidity and mortality. However, there are no objective biomarkers to diagnose AECOPD. Methods We used multiple reaction monitoring mass spectrometry quantify 129 distinct proteins plasma samples from patients with COPD. This analytical approach was first performed a biomarker cohort hospitalized AECOPD (Cohort A, n = 72). Proteins differentially expressed between convalescent states were...
Primary atopic disorders are a group of inborn errors immunity that skew the immune system toward severe allergic disease. Defining biology underlying these extreme monogenic phenotypes reveals shared mechanisms common polygenic disease and identifies potential drug targets. Germline gain-of-function (GOF) variants in JAK1 cause atopy eosinophilia. Modeling JAK1GOF (p.A634D) variant both zebrafish human induced pluripotent stem cells (iPSCs) revealed enhanced myelopoiesis. RNA-Seq whole...
Background Monogenic conditions that disrupt proper development and/or function of the immune system are termed inborn errors immunity (IEIs), also known as primary immunodeficiencies. Patients with IEIs often suffer from other manifestations in addition to infection, and allergic inflammation is an increasingly recognized feature these conditions. Methods We performed a retrospective analysis presenting reported USIDNET registry. Our inclusion criteria comprised patients monogenic cause for...
A highly-multiplexed LC-ESI-multiple reaction monitoring-MS-based assay is developed for the identification of coronary artery disease (CAD) biomarkers in human plasma.The used to measure 107 stable isotope labeled peptide standards and native peptides from 64 putative cardiovascular diseases tryptic digests plasma subjects with (n = 70) without 45) angiographic evidence CAD no subsequent mortality during follow-up.Extensive computational statistical analysis reveals six proteins associated...