A5045591464- Microtubule and mitosis dynamics
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Photosynthetic Processes and Mechanisms
- Fish Biology and Ecology Studies
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- Plant Gene Expression Analysis
- Animal Genetics and Reproduction
- Asthma and respiratory diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic diversity and population structure
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Protist diversity and phylogeny
- Reproductive biology and impacts on aquatic species
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Viral-associated cancers and disorders
- Peptidase Inhibition and Analysis
Peking University
2024
Peking University Cancer Hospital
2024
Center for Excellence in Molecular Plant Sciences
2023
Chinese Academy of Sciences
2017-2023
Jinan University
2023
University of Bonn
2022-2023
Huazhong Agricultural University
2019-2021
Institute of Zoology
2017
China Medical University
2010
Methylations on nucleosomal histones play fundamental roles in regulating eukaryotic transcription. Jumonji C domain-containing histone demethylases (JMJs) dynamically control the level of methylations. However, how JMJ activity is generally regulated unknown. We found that tricarboxylic acid cycle-associated enzyme α-ketoglutarate (α-KG) dehydrogenase (KGDH) entered nucleus, where it interacted with various JMJs to regulate α-KG-dependent demethylations by JMJs, and thus controlled...
The γ-tubulin ring complex (γ-TuRC) is a structural template for de novo microtubule assembly from α/β-tubulin units. isolated vertebrate γ-TuRC assumes an asymmetric, open structure deviating geometry, suggesting that closure may underlie regulation of nucleation. Here, we isolate native γ-TuRC-capped microtubules Xenopus laevis egg extract nucleated through the RanGTP-induced pathway spindle and determine their cryo-EM structure. Intriguingly, minus end-bound only partially closed...
T helper type 9 (Th9) cells, a subpopulation of CD4+ play critical role in the pathogenesis allergic airway inflammation. However, it remains unknown whether mTORC2 regulates Th9 differentiation or function during inflammation.T-cell-specific Rictor-deficient mice, mouse model inflammation induced by ovalbumin (OVA) sensitization and adoptive transfer were used to address roles The vitro induction, multiple colors flow cytometry, real-time PCR, Western blots investigate molecular effects...
Centrosomes represent main microtubule organizing centers (MTOCs) in animal cells. Their duplication S-phase enables the establishment of two MTOCs M-phase that define poles spindle and ensure equal distribution chromosomes centrosomes to daughter While key functions many centrosomal proteins have been addressed RNAi experiments chronic knockdown, knockout with complete loss function all cells enable quantitative analysis cellular phenotypes at cell-cycle stages. Here, we show centriolar...
Hybrid sterility has been widely observed in fish species; however, the molecular mechanisms of hybrid are not well understood. In this work, we compared reproductive development yellow catfish (Pelteobagrus fulvidraco, PF) and fulvidraco ♀ × Pelteobagrus vachelli ♂) from embryo to adult at cellular levels. Firstly, developed a pair genetic markers efficiently distinguish PF PV vachelli) their interspecific hybrid. was catfish, as indicated by germ cell-less phenotype defective gonadal...
Pemigatinib, a pan-FGFR inhibitor, is approved to treat intrahepatic cholangiocarcinoma (ICC) harboring FGFR2 fusion mutations. Improving its targeting of fusions remains an unmet clinical need due pan selectivity and resistance. Here, we report cholesterol-conjugated DNA/RNA heteroduplex oligonucleotide the chimeric site in FGFR2-AHCYL1 (F-A Cho-HDO) that accumulates ICC through endocytosis low-density lipoprotein receptor (LDLR), which highly expressed both human murine ICC. F-A Cho-HDO...
Abstract The nuclear protein SART1 has been associated with pre-mRNA splicing but RNAi knockdown results also in defects mitotic progression, centrosome biogenesis and chromosome cohesion. roles of have not characterized detail it remains unclear whether functions mitosis directly or indirectly via splicing. Here, we identify as a direct, mitosis-specific microtubule-associated protein. downregulation human cells leads to spindle assembly reduced microtubule dynamics, lack end-on attachment,...