PETbox4 is a new, fully tomographic bench top PET scanner dedicated to high sensitivity and resolution imaging of mice. This manuscript characterizes the performance prototype system using National Electrical Manufacturers Association NU 4-2008 standards, including studies sensitivity, spatial resolution, energy scatter fraction, count-rate image quality. The also compared with PETbox, previous generation limited angle tomography system. consists four opposing flat-panel type detectors...

Oncogenic kinase activity and the resulting aberrant growth survival signaling are a common driving force of cancer. Accordingly, many successful molecularly targeted anticancer therapeutics directed at inhibiting activity. To assess in minute patient samples, we have developed an immunocapture-based vitro assay on integrated polydimethylsiloxane microfluidics platform that can reproducibly measure from as few 3,000 cells. For this platform, adopted standard radiometric (32)P-ATP-labeled...

HiPET is a recently developed prototype preclinical PET scanner dedicated to high sensitivity and resolution molecular imaging. The system employs phoswich depth of interaction (DOI) detector design, which also allows identification the large majority cross layer crystal scatter (CLCS) events. This work evaluates its performance characteristics following National Electrical Manufacturers Association (NEMA) NU4-2008 protocol. consists twenty flat panel type detectors arranged in two rings....

PETbox is a low cost bench top preclinical PET scanner dedicated to pharmacokinetic and pharmacodynamic mouse studies. A prototype system was developed at our institute, this manuscript characterizes the performance of system.The detector consists 20 × 44 bismuth germanate crystal array with thickness 5 mm cross-section size 2.05 mm. Two such detectors are placed facing each other spacing cm, forming dual-head geometry optimized for imaging mice. The kept stationary during scan, making...

G8 is a benchtop integrated PET/CT scanner dedicated to high-sensitivity and high-resolution imaging of mice. This work characterizes its National Electrical Manufacturers Association NU 4-2008 performance where applicable also assesses the basic CT subsystem. <b>Methods:</b> The PET subsystem in consists 4 flat-panel detectors arranged boxlike geometry. Each panel 2 modules 26 × pixelated bismuth germanate scintillator array with individual crystals measuring 1.75 7.2 mm. crystal arrays are...

An integrated β-camera and microfluidic chip was developed that is capable of quantitative imaging glycolysis radioassays using (18)F-FDG in small cell populations down to a single cell. This paper demonstrates the system enables digital control measurements B-Raf(V600E)-mutated melanoma lines response specific B-Raf inhibition.The uses position-sensitive avalanche photodiode detect charged particle-emitting probes within chip. The calibrated, linearity measured 4 different (M257, M202,...

PETbox is a low-cost benchtop PET scanner dedicated to high throughput preclinical imaging that currently under development at our institute. This paper presents the design and characterization of detectors are used in system. In this work, bismuth germanate scintillator was for detector, taking advantage its stopping power, photoelectric event fraction, lack intrinsic background radiation low cost. The detector block segmented into pixelated array consisting 20 × 44 elements, with crystal...

A beta camera has been developed that allows planar imaging of the spatial and temporal distribution particles using a 14 × mm2 position sensitive avalanche photodiode (PSAPD). This system, which we call Betabox, can be directly coupled to microfluidic chips designed for cell incubation or other biological applications. Betabox cellular uptake molecular probes labeled with charged particle emitters such as 18F inside these chips. In this work, investigate quantitative capabilities particles,...

This paper introduces a mouse atlas registration system (MARS), composed of stationary top-view x-ray projector and side-view optical camera, coupled to algorithm. uses the images guide fully automatic co-registration with each subject, in order provide anatomical reference for small animal molecular imaging systems such as positron emission tomography (PET). To facilitate registration, statistical that accounts inter-subject variations was constructed based on 83 organ-labeled...

Molecules under strong or ultra-strong light-matter coupling present an intriguing route to modify chemical structure, properties, and reactivity. A rigorous theoretical treatment of such systems requires handling matter photon degrees freedom on equal quantum mechanical footing. In the regime molecular electronic one a few molecules, it is desirable treat using tools ab initio chemistry, yielding approach referred as cavity electrodynamics (ai-QED), where are treated at level...

This paper develops an automated vascular access system (A-VAS) with novel vision-based vein and needle detection methods real-time pressure feedback for murine drug delivery. Mouse tail injection is a routine but critical step preclinical imaging applications. Due to the small diameter external disturbances such as hair, pigmentation, scales, identifying location difficult manual injections usually result in poor repeatability. To improve accuracy, consistency, safety, processing time,...

Precisely controlled impurity doping is of fundamental significance in modern semiconductor technologies. Desired physical properties are often achieved at concentrations well below parts per million level. For emergent two-dimensional semiconductors, development reliable strategies hindered by the inherent difficulty identifying and quantifying impurities such a dilute limit where absolute number atoms to be detected insufficient for common analytical techniques. Here we report rapid...

This paper reports on the geometric calibration procedure of a bench-top, high-speed, and continuous-rotation preclinical computed tomography (CT) scanner that has been developed at our institution. A full 13-parameter model image formation process is described. Once calibrated, used to modify projection data sets - standardization operation so they appear come from an ideal system. technique advantage standardized scans can be fed any reconstruction algorithm since no awareness actual...

Novel molecular imaging applications increasingly involve studies where very low amount of activity is present. This operation point can be challenging in terms image quality especially when the intrinsic detector from scintillators such as LSO considered. crystals contain <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">176</sup> Lu which emits β xmlns:xlink="http://www.w3.org/1999/xlink">−</sup> ; particles followed by γ photons, resulting...

PETbox is designed to be a low cost and easy use bench top small animal PET scanner dedicated for high throughput quantitative pharmacokinetic pharmacodynamic studies. To achieve this goal, the integrated with complete management system that provides life support including reproducible positioning, temperature control, anesthesia, real-time monitoring of respiration pathogen barrier. This approach minimizes overall complexity preclinical imaging should enable non-imaging scientists embrace...

A new device is being developed that will allow imaging of the distribution charged particles in microfluidic circuits, using a scintillator, lens and charge coupled (CCD). Microfluidic chips are an emerging technology facilitate study molecular processes pico-liter levels finely controlled manner. The ability to quantify low amounts radioactivity chip provide researchers with platform investigate in-vitro environment. detector system consists plastic C-mount compact lens, Sony ICX285AL...

A new device has been developed that allows imaging of charged particle emitting probes in a microfluidic circuit using position sensitive avalanche photodiode (PSAPD). Microfluidic chips are an emerging technology have used applications such as the synthesis molecular and incubation live cell cultures chambers. This works by direct contact PSAPD detector with substrate layer chip provides 2-dimensional images distribution beta over time. The spatial resolution for was evaluated <sup...

A new device is being developed that will allow imaging of the distribution charged particles in microfluidic circuits, using position sensitive avalanche photodiodes (PSAPD). Microfluidic chips are an emerging technology facilitate synthesis and study molecular probes. These poly-dimethylsiloxane (PDMS) low cost to custom design produce, their development/prototyping cycles relatively short. They can contain a variety microcircuitry microwells for manipulating nanoliter volumes solutions....

Microfluidic chips are an emerging technology that facilitates the study of molecular processes in nano-liter levels a finely controlled manner. A prototype imaging system capable detecting and quantifying very small amounts beta particles microfluidic chip, utilizing scintillator, optical lens, Charge Coupled Device (CCD), was developed proof concept previously demonstrated [1]. In this study, we optimized design parameters, such as CCD binning types scintillators, evaluated performance for...

A new system is being developed to allow imaging of charged particles in microfluidic chip using position sensitive avalanche photodiodes (PSAPD). The was created by placing the PSAPD detector with close proximity PDMS microchip. therefore capable quantifying tiny amounts these radiolabeled probes over time. can be used for direct detection positrons and other particles. In our studies, performance has been carried out. gradient solution containing radioactive positron emitting...

BetaBox, a direct detection beta camera utilizing 13.5×13.5mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> active area position sensitive avalanche photodiode (PSAPD) (Radiation Monitoring Devices) is currently being developed to image distributions of charged particles in microfluidic chips. The previously reported signal processing chain incorporated charge amplifiers (Cremat Inc., CR-110) the frontend readout design. Charge...

Small animal anatomical imaging with x-ray computed tomography (CT) in cone-beam geometry suffers from inaccuracies the measured attenuation coefficient due to acceptance of scattered radiation. This work seeks estimate magnitude and effect scatter a commercial microCT scanner both projections reconstructed images. The scatter-to-primary ratio (SPR) is estimated using beam stop method simulation. We also make initial attempts quantitate amount inaccuracy image values under different...

Information on the transport and phosphorylation rate constants (k1,k2,k3,k4,Ki) of a tracer reflects biological state cells. A microfluidic cell culture chip coupled with PSAPD camera (MF-PSAPD) has been developed to give continuous measurements radioactivity in individual wells. However, constant infusion (CI) PET tracers through chambers would high background activity due relatively large volume space infusing medium wells cultured cells that compromise ability setup estimate k values....

Spatial resolution and noise are strongly related properties therefore meaningful evaluations of imaging systems reconstruction algorithms should always include estimates both. We have evaluated the performance a statistical maximum posteriori probability (MAP) algorithm in terms contrast recovery signal to ratio (SNR) comparison filtered-backprojection (FBP) on microPET Focus220 scanner. Printed <sup xmlns:mml="http://www.w3.org/1998/Math/MathML"...