A5102885542- Nanopore and Nanochannel Transport Studies
- Fuel Cells and Related Materials
- Electrostatics and Colloid Interactions
- Ion-surface interactions and analysis
- Geophysical and Geoelectrical Methods
- Advanced biosensing and bioanalysis techniques
- Membrane Separation Technologies
- Anodic Oxide Films and Nanostructures
- Electrokinetic Soil Remediation Techniques
- RNA Interference and Gene Delivery
- Advancements in Semiconductor Devices and Circuit Design
- Membrane-based Ion Separation Techniques
- Semiconductor materials and devices
- Groundwater flow and contamination studies
- Microfluidic and Capillary Electrophoresis Applications
- Quantum-Dot Cellular Automata
University of Ottawa
2012-2023
Nanopore fabrication by controlled breakdown (CBD) overcomes many of the challenges traditional nanofabrication techniques, reliably forming solid-state nanopores sub-2 nm in size a low-cost and scalable way for nucleic acid analysis applications. Herein, kinetics thin dielectric membranes immersed liquid environment are investigated order to gain deeper insights into mechanism nanopore formation high electric fields. For various conditions, we demonstrate that time is Weibull-distributed,...
We report the use of an array electrically gated ~200 nm solid-state pores as nanofluidic transistors to manipulate capture and passage DNA. The devices are capable reversibly altering rate DNA by over 3 orders magnitude using sub-1 V biasing a gate electrode. This efficient gating originates from counter-balance electrophoresis electroosmosis, revealed quantitative numerical simulations. Such reversible electronically tunable biomolecular switch may be used nucleic acid delivery in fluidic...
Using a solid-state nanopore to measure the concentration of clinically relevant target analytes, such as proteins or specific DNA sequences, is major goal research. This usually achieved by measuring capture rate analyte through pore. However, progress hindered sources systematic error that are beyond level control currently achievable with state-of-the-art nanofabrication techniques. In this work, we show process nanopores subject significant variability, both within individual over time...
We demonstrate the ability to slow DNA translocations through solid‐state nanopores by interfacing trans side of membrane with gel media. In this work, we focus on two reptation regimes: when molecule is flexible length scale a pore, and behaves as persistent segments in tight pores. The first regime investigated using agarose gels, which produce very wide distribution translocation times for 5 kbp dsDNA fragments, spanning over three orders magnitude. second attained polyacrylamide can...
The fabrication of individual nanopores in metallized dielectric membranes using controlled breakdown directly solution is described. Nanopores as small 1.5‐nm diameter are fabricated Au‐coated silicon nitride immersed 1 m KCl by subjecting them to high electric fields. physical and electrical characteristics produced with this method presented. translocation short single‐stranded DNA molecules demonstrated through such nanopore devices without further passivation the metallic surface....
We present a thorough exploration of nanopore growth under electrical stress in electrolyte solution, and demonstrate that despite their superficial similarities, formation by controlled breakdown (CBD) moderate voltage are fundamentally different processes. In particular, we unlike the CBD process, is primarily driven level ionic current passing through nanopore, rather than strength electric field generating current, enlargement has much weaker pH dependence does pore formation....
Solid-state nanopores, nm-sized holes in thin, freestanding membranes, are powerful single-molecule sensors capable of interrogating a wide range target analytes, from small molecules to large polymers. Interestingly, due their high spatial resolution, nanopores can also identify tags on long polymers, making them an attractive option as the reading element for molecular information storage strategies. To fully leverage compact and robust nature solid-state however, they will need be...