A5024282851- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Ion-surface interactions and analysis
- Microfluidic and Capillary Electrophoresis Applications
- Electrostatics and Colloid Interactions
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Anodic Oxide Films and Nanostructures
- Advancements in Semiconductor Devices and Circuit Design
- Geophysical and Geoelectrical Methods
- Semiconductor materials and devices
- Advanced Biosensing Techniques and Applications
- Membrane-based Ion Separation Techniques
- Particle physics theoretical and experimental studies
- Membrane Separation Technologies
- Advanced Surface Polishing Techniques
- Laser-induced spectroscopy and plasma
- Neutrino Physics Research
- Fuel Cells and Related Materials
- Laser Material Processing Techniques
- Geophysical Methods and Applications
- Astrophysics and Cosmic Phenomena
- Innovative Microfluidic and Catalytic Techniques Innovation
- Laser-Plasma Interactions and Diagnostics
- Groundwater flow and contamination studies
University of Ottawa
2015-2024
GlobalFoundries (United States)
2020-2023
Saint-Vincent Hospital
2022
University of Warwick
2013
Colorado State University
2011
BT Research
2002
The T2K experiment is a long baseline neutrino oscillation experiment. Its main goal to measure the last unknown lepton sector mixing angle θ13 by observing νe appearance in νμ beam. It also aims make precision measurement of known parameters, Δm232 and sin22θ23, via disappearance studies. Other goals include various cross-section measurements sterile searches. uses an intense proton beam generated J-PARC accelerator Tokai, Japan, composed beamline, near detector complex (ND280), far...
Nanofabrication techniques for achieving dimensional control at the nanometer scale are generally equipment-intensive and time-consuming. The use of energetic beams electrons or ions has placed fabrication nanopores in thin solid-state membranes within reach some academic laboratories, yet these tools not accessible to many researchers poorly suited mass-production. Here we describe a fast simple approach fabricating single nanopore down 2-nm size with sub-nm precision, directly solution, by...
We demonstrate the automated and reproducible fabrication of sub-2-nm nanopores in 10-nm thick silicon nitride membranes, through controlled dielectric breakdown solution. Our results reveal that under appropriate conditions, can be fabricated with a size no larger than 2.0 ± 0.5-nm diameter for sample N = 23 nanopores, an average standard deviation 1.3 0.6-nm. The dimensions these are confirmed by using individual translocating DNA molecules as molecular rulers. show 2.0-nm 2.1-nm nanopore...
Biological and solid-state nanometer-scale pores are the basis for numerous emerging analytical technologies use in precision medicine. We developed Modular Single-Molecule Analysis Interface (MOSAIC), an open source analysis software that improves accuracy throughput of nanopore-based measurements. Two key algorithms implemented: ADEPT, which uses a physical model nanopore system to characterize short-lived events do not reach their steady-state current, CUSUM+, version cumulative sum...
Nanopore fabrication by controlled breakdown (CBD) overcomes many of the challenges traditional nanofabrication techniques, reliably forming solid-state nanopores sub-2 nm in size a low-cost and scalable way for nucleic acid analysis applications. Herein, kinetics thin dielectric membranes immersed liquid environment are investigated order to gain deeper insights into mechanism nanopore formation high electric fields. For various conditions, we demonstrate that time is Weibull-distributed,...
Single-molecule counting is the most accurate and precise method for determining concentration of a biomarker in solution leading to emergence digital diagnostic platforms enabling precision medicine. In principle, solid-state nanopores-fully electronic sensors with single-molecule sensitivity-are well suited task. Here we present immunoassay scheme capable reliably quantifying target protein complex biofluids that overcomes specificity, sensitivity, consistency challenges associated use...
To reduce unwanted variation in the passage speed of DNA through solid-state nanopores, we demonstrate nanoscale preconfinement translocating molecules using an ultrathin nanoporous silicon nitride membrane separated from a single sensing nanopore by cavity. We present comprehensive experimental and simulation results demonstrating that presence integrated nanofilter within distances pore eliminates dependence molecular time distributions on size, revealing global minimum coefficient time....
We demonstrate precise positioning of nanopores fabricated by controlled breakdown (CBD) on solid-state membranes spatially varying the electric field strength with localized membrane thinning. show 100 × nm2 precision in standard SiN x (30-100 nm thick) after selective thinning as little 25% a helium ion beam. Control over nanopore position is achieved through strong dependence field-driven CBD mechanism thickness. Confinement pore formation to thinned region confirmed TEM imaging and...
Using a solid-state nanopore to measure the concentration of clinically relevant target analytes, such as proteins or specific DNA sequences, is major goal research. This usually achieved by measuring capture rate analyte through pore. However, progress hindered sources systematic error that are beyond level control currently achievable with state-of-the-art nanofabrication techniques. In this work, we show process nanopores subject significant variability, both within individual over time...
The T2K experiment studies oscillations of an off-axis muon neutrino beam between the J-PARC accelerator complex and Super-Kamiokande detector. Special emphasis is placed on measuring mixing angle theta_13 by observing electron appearance via sub-dominant to oscillation, searching for CP violation in lepton sector. includes a sophisticated, off-axis, near detector, ND280, situated 280 m downstream production target order measure properties understand better interactions at energy scale below...
The promise of portable diagnostic devices relies on three basic requirements: comparable sensitivity to established platforms, inexpensive manufacturing and cost operations, the ability survive rugged field conditions. Solid state nanopores can meet all these requirements, but achieve high yields at low costs, assays must be tolerant fabrication imperfections nanopore enlargement during operation. This paper presents a model for molecular engineering techniques that meets goals with aim...
Using 3D Finite-Difference-Time-Domain simulations, we study the morphology of laser-created damage inside fused silica. Among competing effects limiting intensity in dielectric, find most important is pulse defocusing by plasma lens, partially balanced Kerr effect. Less are collisional energy dissipation and laser depletion multi-photon absorption. We also found that profile generated asymmetrical transverse cross-section, with extended along direction perpendicular to polarization.
We demonstrate the ability to slow DNA translocations through solid‐state nanopores by interfacing trans side of membrane with gel media. In this work, we focus on two reptation regimes: when molecule is flexible length scale a pore, and behaves as persistent segments in tight pores. The first regime investigated using agarose gels, which produce very wide distribution translocation times for 5 kbp dsDNA fragments, spanning over three orders magnitude. second attained polyacrylamide can...
On-chip microvalves regulate electrical and fluidic access to an array of nanopores integrated within microfluidic networks. This configuration allows for on-chip sequestration biomolecular samples in various flow channels analysis by independent nanopores. Please note: The publisher is not responsible the content or functionality any supporting information supplied authors. Any queries (other than missing content) should be directed corresponding author article.
The fabrication of individual nanopores in metallized dielectric membranes using controlled breakdown directly solution is described. Nanopores as small 1.5‐nm diameter are fabricated Au‐coated silicon nitride immersed 1 m KCl by subjecting them to high electric fields. physical and electrical characteristics produced with this method presented. translocation short single‐stranded DNA molecules demonstrated through such nanopore devices without further passivation the metallic surface....
Single-molecule detection methods are becoming increasingly important for diagnostic applications. Practical early of disease requires sensitivity down to the level single copies targeted biomarkers. Of candidate technologies that can address this need, solid-state nanopores show great promise as digital sensors single-molecule detection. Here, we present work detailing use downstream a polymerase chain reaction (PCR)-based assay targeting group A streptococcus (strep A), which be readily...
Elucidating the kinetics of DNA passage through a solid-state nanopore is fertile field research, and mechanisms for controlling capture, passage, trapping biopolymers are likely to find numerous technological applications. Here we present nanofiltered device which forms an entropic cage following first nanopore, translocated DNA, permitting recapture subsequent reanalysis investigation under confinement. We characterize properties this nanodevice by driving individual polymers into...
We present a thorough exploration of nanopore growth under electrical stress in electrolyte solution, and demonstrate that despite their superficial similarities, formation by controlled breakdown (CBD) moderate voltage are fundamentally different processes. In particular, we unlike the CBD process, is primarily driven level ionic current passing through nanopore, rather than strength electric field generating current, enlargement has much weaker pH dependence does pore formation....
Nanopores are versatile single-molecule sensors that being used to sense increasingly complex mixtures of structured molecules with applications in molecular data storage and disease biomarker detection. However, increased complexity presents additional challenges the analysis nanopore data, including more translocation events rejected for not matching an expected signal structure a greater risk selection bias entering this event curation process. To highlight these challenges, here, we...
We examine the effect of cluster size on interaction Ar$_{55}$-Ar$_{2057}$ with intense extreme ultraviolet (XUV) pulses, using a model we developed earlier that includes ionization via collisional excitation as an intermediate step. find dynamics these irradiated clusters is dominated by collisions. Larger are more highly collisional, produce higher charge states, and do so rapidly than smaller clusters. Higher states produced collisions found to reduce overall photon absorption, since...
Nanoscale preconfinement of DNA has been shown to reduce the variation passage times through solid-state nanopores. Preconfinement previously achieved by forming a femtoliter-sized cavity capped with highly porous layer nanoporous silicon nitride (NPN). This was formed sealing NPN nanofilter membrane against substrate chip using water vapor delamination. Ultimately, this method fabrication cannot keep consistent spacing between filter and nanopore due thermal fluctuations wrinkles in...
Characterization of DNA nanostructures using solid-state nanopores: Single-file and folded translocations give insights on polymer dimensions rigidity.