A5022663913- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Bioeconomy and Sustainability Development
- biodegradable polymer synthesis and properties
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
Hubei University
2022-2025
High Heterologous PHB Production and Continuous Anaerobic Ethanol Fermentation using Zymomonas mobilis.
Lactate is the precursor for polylactide. In this study, a lactate producer of Z. mobilis was constructed by replacing ZMO0038 with LmldhA gene driven strong promoter P adhB , ZMO1650 native pdc tet, and another copy to divert carbon from ethanol D-lactate. The resultant strain ZML-pdc-ldh produced 13.8 ± 0.2 g/L 16.9 0.3 using 48 glucose. production further investigated after fermentation optimization in pH-controlled fermenters. 24.2 0.6 12.9 0.8 as well 36.2 1.0 40.3 ethanol, resulting...
Xylitol is a sugar–alcohol compound with broad applications in fields such as the food, dental, and pharmaceutical sectors. Although xylitol biosynthesis has gained attention, current strategy for industrial production majorly relies on chemical hydrogenation of xylose, which energy-intensive environmentally harmful. In this study, toxicity toward Escherichia coli was first examined, result demonstrated that robust against at 150 g/L. Genes encoding xylose reductases from different...
Bio-based xylonic acid produced from inexpensive lignocellulosic biomass has enormous market potential and enhances the overall economic benefits of biorefinery processes. In this study, introduction genes encoding xylose dehydrogenase driven by promoter Ppdc into Z. mobilis using a plasmid vector resulted in accumulation at titer 16.8 ± 1.6 g/L. To achieve stable production, gene cassette for production was integrated genome chromosomal locus ZMO0038 ZMO1650 endogenous type I-F CRISPR-Cas...
Due to the complicated metabolic and regulatory networks of l-serine biosynthesis degradation, microbial cell factories for production using non-model microorganisms have not been reported. In this study, a combination synthetic biology process optimization were applied in an ethanologenic bacterium Zymomonas mobilis production. By blocking degradation pathway while introducing exporter EceamA from E. coli, titer recombinant Z. was increased 15.30 mg/L 62.67 mg/L. It further 260.33 after...