A5025814993- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- Cancer Genomics and Diagnostics
- Biochemical Acid Research Studies
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Metabolism and Genetic Disorders
- Alcoholism and Thiamine Deficiency
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Microbial Metabolic Engineering and Bioproduction
- Methemoglobinemia and Tumor Lysis Syndrome
- Wine Industry and Tourism
- Liver Disease Diagnosis and Treatment
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Protein Degradation and Inhibitors
Shandong University
2015-2025
Qilu Hospital of Shandong University
2023
Peking University
2018-2022
Beijing National Laboratory for Molecular Sciences
2020-2022
Center for Life Sciences
2018-2022
King Center
2018
King University
2018
State Key Laboratory of Microbial Technology
2015
Transcriptome-wide identification of RNA-binding proteins (RBPs) is a prerequisite for understanding the posttranscriptional gene regulation networks. However, proteomic profiling RBPs has been mostly limited to polyadenylated mRNA-binding proteins, leaving on nonpoly(A) RNAs, including most noncoding RNAs (ncRNAs) and pre-mRNAs, largely undiscovered. Here we present click chemistry-assisted RNA interactome capture (CARIC) strategy, which enables unbiased RBPs, independent polyadenylation...
Abstract Diacetyl, a high value product that can be extensively used as food ingredient, could produced from the non-enzymatic oxidative decarboxylation of α-acetolactate during 2,3-butanediol fermentation. In this study, biosynthetic pathway in Enterobacter cloacae subsp. dissolvens strain SDM, good candidate for microbial production, was reconstructed diacetyl production. To enhance accumulation precursor diacetyl, decarboxylase encoding gene ( budA ) knocked out SDM. Subsequently, two...
Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes. However, metabolic is not applicable prokaryotes, which the complexity distinctness gene regulation largely remain to be explored. Here, we report 2'-deoxy-2'-azidoguanosine (AzG) nucleoside compatible bacterial RNAs. With AzG, develop...
Protein lipoylation is a post-translational modification of emerging importance in both prokaryotes and eukaryotes. However, labeling large-scale profiling protein remain challenging. Here, we report the development iLCL (iodoacetamide-assisted lipoate-cyclooctyne ligation), chemoselective reaction that enables chemical tagging lipoylation. We demonstrate cyclic disulfide lipoamide but not linear disulfides can selectively react with iodoacetamide to produce sulfenic acid, which be...
A comprehensive identification of RNA-binding proteins (RBPs) is key to understanding the posttranscriptional regulatory network in cells. widely used strategy for RBP capture exploits polyadenylation [poly(A)] target RNAs, which mostly occurs on eukaryotic mature mRNAs, leaving most binding non-poly(A) RNAs unidentified. Here we describe detailed procedures a recently reported method termed click chemistry-assisted RNA-interactome (CARIC), enables transcriptome-wide both poly(A) and RBPs by...
Abstract Metabolic labeling with clickable noncanonical amino acids has enabled nascent proteome profiling, which can be performed in a cell‐type‐specific manner. However, proteomics an intercellular communication‐dependent manner remains challenging. Here we develop communication‐activated profiling of protein expression (CAPPEX), integrates the LuxI/LuxR quorum sensing circuit method to enable selective click‐labeling newly synthesized proteins specific bacterium upon receiving chemical...
Abstract Protein lipoylation is a post‐translational modification of emerging importance in both prokaryotes and eukaryotes. However, labeling large‐scale profiling protein remain challenging. Here, we report the development iLCL (iodoacetamide‐assisted lipoate‐cyclooctyne ligation), chemoselective reaction that enables chemical tagging lipoylation. We demonstrate cyclic disulfide lipoamide but not linear disulfides can selectively react with iodoacetamide to produce sulfenic acid, which be...
Executing glycan editing at a molecular level not only is pivotal for the elucidation of complicated mechanisms involved in glycan-relevant biological processes but also provides promising solution to potentiate disease therapy. However, precision control modification or glyco-editing on selected glycoprotein by far grand challenge. Of note preserve intact cellular landscape, which preserved after events are completed. We report herein versatile, traceless methodology customizing glycoforms...
Abstract Metabolic labeling with clickable noncanonical amino acids has enabled nascent proteome profiling, which can be performed in a cell‐type‐specific manner. However, proteomics an intercellular communication‐dependent manner remains challenging. Here we develop communication‐activated profiling of protein expression (CAPPEX), integrates the LuxI/LuxR quorum sensing circuit method to enable selective click‐labeling newly synthesized proteins specific bacterium upon receiving chemical...
Background: As two diseases with rapidly increasing incidence, the molecular linkages between obesity and breast cancer (BC) are intriguing. Overall, may be a negative prognostic factor for BC.Methods: Single-cell RNA-sequencing (scRNA-seq) was performed on tumor tissues from 6 obese non-obese BC patients. The induced macrophages were used to evaluate regulation role of NR1H3 FABP4. RNA sequencing, qPCR western blot applied measure expression levels co-culture, cell proliferation migration...
<title>Abstract</title> As two diseases with rapidly increasing incidence, the molecular linkages between obesity and breast cancer (BC) are intriguing. Overall, may be a negative prognostic factor for BC. Single-cell RNA-sequencing (scRNA-seq) was performed on tumor tissues from 6 obese non-obese BC patients. With 48,033 cells analyzed, we found heterogeneous epithelium microenvironment in these lean Interestingly, obesity-associated epithelial exhibited specific expression signatures which...