A5033185985- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Chalcogenide Semiconductor Thin Films
- X-ray Diffraction in Crystallography
- Quantum Dots Synthesis And Properties
- Crystallization and Solubility Studies
- Various Chemistry Research Topics
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Silicon and Solar Cell Technologies
- Chemical synthesis and alkaloids
- Alkaloids: synthesis and pharmacology
- Microbial Metabolic Engineering and Bioproduction
- Advanced Semiconductor Detectors and Materials
- RNA regulation and disease
- Environmental Monitoring and Data Management
- Thin-Film Transistor Technologies
- Phytochemistry and Bioactivity Studies
- DNA and Nucleic Acid Chemistry
- Supramolecular Self-Assembly in Materials
- Advanced Electron Microscopy Techniques and Applications
- Photovoltaic Systems and Sustainability
Rigaku (United States)
2013-2025
Rigaku (Japan)
2025
Veeco (United States)
2005-2024
Instituto Nacional de Investigación Agropecuaria
2019
The University of Texas at Austin
2005-2012
Institut de Biologie Moléculaire et Cellulaire
2009-2011
University of Miami
2001-2004
Antec (Germany)
2001-2003
University of Tartu
2001
CALF-20, a Zn-triazolate-based metal–organic framework (MOF), is one of the most promising adsorbent materials for CO2 capture. However, competitive adsorption water severely limits its performance when relative humidity (RH) exceeds 40%, limiting potential implementation CALF-20 in practical settings where saturated with moisture, such as postcombustion flue gas. In this work, three newly designed MOFs related to denoted NU-220, CALF-20M-w, and CALF-20M-e that feature hydrophobic...
DEAD-box proteins are ubiquitous in RNA metabolism and use ATP to mediate conformational changes. These have been suggested a fundamentally different mechanism from the related DNA helicases, generating local strand separation while remaining tethered through additional interactions with structured RNAs RNA-protein (RNP) complexes. Here, we provide critical test of this model by measuring number molecules hydrolyzed as they separate short helices characteristic (6–11 bp). We show that...
The mitochondrial DEAD-box proteins Mss116p of Saccharomyces cerevisiae and CYT-19 Neurospora crassa are ATP-dependent helicases that function as general RNA chaperones. helicase core each protein precedes a C-terminal extension basic tail, whose structural role is unclear. Here we used small-angle X-ray scattering to obtain solution structures the full-length series deletion mutants. We find two domains have preferred relative orientation in open state without substrates, visualize...
Hedgehog signalling plays a fundamental role in the control of metazoan development, cell proliferation and differentiation, as highlighted by fact that its deregulation is associated with development many human tumours. SUFU an essential intracellular negative regulator mammalian acts binding modulating activity GLI transcription factors. Despite central importance, little known about regulation nature SUFU–GLI interaction. Here, crystal small-angle X-ray scattering structures full-length...
Rigaku ran a series of practical schools to address the loss crystallographic training opportunities resulting from cancellation conventional around world due COVID-19 pandemic. With return normalcy, we stopped holding schools. However, in last year number requests were made reprise lectures. This year, one school but took different approach. Rather than performing lectures live, prerecorded them with intention using for future on-demand school. We held live Q&A sessions after capture...
The molecular structures of an anhydrous and dihydrate forms a 24-atom triazine macrocycle containing proline methine hydrazine residues, were determined by MicroED (Single Crystal Electron Diffraction, ScED, 3DED). whole powder pattern profile decomposition (WPPD) Pawley refinement the X-ray Powder Pattern (RT, XRPD) confirms two crystalline in sample indicates one or more additional unidentified states. is protonated at triazines (pKa ∼6) trifluoroacetic acid (TFA, pKa 0.25) to yield...
The DEAD-box protein CYT-19 functions in the folding of several group I introns vivo and a diverse set II RNAs vitro. Recent work using Tetrahymena ribozyme demonstrated that possesses second RNA-binding site, distinct from unwinding active which enhances activity by binding nonspecifically to adjacent RNA structure. Here, we probe region responsible for constructing C-terminal truncation variant lacks 49 amino acids terminates at domain boundary, as defined limited proteolysis. This...
Multidrug-resistant Staphylococcus aureus infections pose a significant threat to human health. Antibiotic resistance is most commonly propagated by conjugative plasmids like pLW1043, the first vancomycin-resistant S. vector identified in humans. We present molecular basis for transmission nicking enzyme (NES), which essential transfer. NES initiates and terminates transfer of that variously confer range drugs, including vancomycin, gentamicin, mupirocin. The N-terminal relaxase–DNA complex...
Along with the adoption of three-dimensional electron diffraction (3D ED/MicroED) as a mainstream tool for structure determination from sub-micron single crystals, questions about best practices regarding each step along workflow, data collection to solutions, arise. In this paper, we discuss three particular aspects 3D ED/MicroED experiment which, after hundreds structures solved in Rigaku’s laboratories, have found be important consider carefully. First, representative model system...
This laboratory previously showed that truncation of the gene for RluD, Escherichia coli pseudouridine synthase responsible synthesis 23S rRNA pseudouridines 1911, 1915, and 1917, blocks formation inhibits growth. We now show RluD mutants at essential aspartate 139 allow these two functions to be separated. In vitro, with replaced by threonine or asparagine is completely inactive. vivo, growth defect could restored transformation an RluD-inactive strain plasmids carrying genes asparagine....
Securines and securamines are cytotoxic alkaloids that contain reactive alkene heterocyclic residues embedded in skeletons comprising four to six oxidized rings. This structural complexity imparts a rich chemistry the isolates but has impeded synthetic access structures nearly three decades since their isolation. We present flexible route eight exemplify skeletal classes of metabolites. The proceeds by modular assembly advanced azides
Escherichia coli pseudouridine synthase RluD makes pseudouridines 1911, 1915, and 1917 in the loop of helix 69 23S RNA. These are most highly conserved ribosomal known. Of 11 synthases E. , only cells lacking have severe growth defects abnormal ribosomes. We determined 2.0 Å structure catalytic domain (residues 77–326), first an RluA family member. The folds into a mainly antiparallel β-sheet flanked by several loops helices. A positively charged cleft that presumably binds RNA leads to Asp...
We have studied the contact formation on CdTe surfaces following technologically applied procedure. The electronic properties of wet chemically etched has been investigated with photoelectron spectroscopy. For characterization morphology, structure, and elemental distribution in layer atomic force microscopy, scanning electron grazing incidence x-ray diffraction, secondary ion mass spectroscopy used. Etching samples performed air an electrochemistry chamber directly attached to UHV system....