A5062206015- RNA modifications and cancer
- Ovarian function and disorders
- Biosensors and Analytical Detection
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- SARS-CoV-2 detection and testing
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Nanofabrication and Lithography Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Protease and Inhibitor Mechanisms
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- Microfluidic and Capillary Electrophoresis Applications
- Virus-based gene therapy research
- RNA Research and Splicing
- Extracellular vesicles in disease
University of Chicago
2023-2024
AstraZeneca (United States)
2020-2023
There is an urgent need for robust and high-throughput methods SARS-CoV-2 detection in suspected patient samples to facilitate disease management, surveillance, control. Although nucleic acid such as reverse transcription polymerase chain reaction (RT-PCR) are the gold standard, during current pandemic, deployment of RT-PCR tests has been extremely slow, key reagents PCR primers RNA extraction kits at critical shortages. Rapid point-of-care viral antigen have previously employed diagnosis...
Abstract Human antigen R (HuR) is a ubiquitously expressed RNA-binding protein, which functions as an RNA regulator. Overexpression of HuR correlates with high grade tumours and poor patient prognosis, implicating it attractive therapeutic target. However, effective small molecule antagonist to for clinical use remains elusive. Here, single domain antibody (VHH) that binds low nanomolar affinity was identified shown inhibit binding RNA. This VHH used engineer TRIM21-based biological PROTAC...
Widespread alterations in RNA alternative splicing (AS) have been identified adult gliomas. However, their regulatory mechanism, biological significance, and therapeutic potential remain largely elusive. Here, using a computational approach with both bulk single cell RNA-sequencing, we uncover prognostic AS signature linked neural developmental hierarchies. Using advanced iPSC glioma models driven by driver mutations, show that this could be enhanced EGFRvIII inhibited situ IDH1 mutation....
Exosome-based therapy has emerged as a promising strategy for addressing diverse disorders, indicating the need further exploration of potential therapeutic effects exosome cargos. This study introduces “enhanced exosomes”, novel type exosomes developed through cell culture system. These specific may become potent agents treating ovarian disorders. In this study, we conducted comparative analysis protein and miRNA cargo compositions enhanced naïve exosomes. Our findings revealed distinct in...
With the aim of increasing protein productivity Chinese hamster ovary (CHO) cells, we sought to generate new CHO hosts with favorable biomanufacturing phenotypes and improved functionality. Here, present an innovative approach enriching host cells a high mitochondrial membrane potential (MMP). Stable transfectant pools clonal cell lines expressing difficult-to-express bispecific molecules generated from MMP-enriched outperformed parental by displaying (1) fed-batch productivity; (2) enhanced...
Citrullination is an important post-translational modification implicated in many diseases including rheumatoid arthritis (RA), Alzheimer's disease, and cancer. Neutrophil mast cells have different expression profiles for protein-arginine deiminases (PADs), ionomycin-induced activation makes them ideal cellular model to study proteins susceptible citrullination. We performed high-resolution mass spectrometry stringent data filtration identify citrullination sites neutrophil treated with...
Exosome-based therapy has emerged as a promising strategy for addressing diverse disorders, indicating the need further exploration of potential therapeutic effects exosome cargos. This study introduces "enhanced exosomes," novel type exosomes developed through cell culture system. These specific may become potent agents treating ovarian disorders. In this study, we conducted comparative analysis protein and miRNA cargo compositions enhanced naïve exosomes. Our findings...