ABSTRACT The potential for removing uranium from contaminated groundwater by stimulating the in situ activity of dissimilatory metal-reducing microorganisms was evaluated a uranium-contaminated aquifer located Rifle, Colo. Acetate (1 to 3 mM) injected into subsurface over 3-month period via an injection gallery composed 20 wells, which installed upgradient series 15 monitoring wells. U(VI) concentrations decreased as little 9 days after acetate initiated, and within 50 had declined below...

The genus Shewanella has been studied since 1931 with regard to a variety of topics relevance both applied and environmental microbiology. Recent years have seen the introduction large number new Shewanella-like isolates, necessitating coordinated review genus. In this work, phylogenetic relationships among known shewanellae were examined using battery morphological, physiological, molecular chemotaxonomic characterizations. This polyphasic taxonomy takes into account all available...

ABSTRACT Contamination of food by Listeria monocytogenes is thought to occur most frequently in food-processing environments where cells persist due their ability attach stainless steel and other surfaces. Once attached these may produce multicellular biofilms that are resistant disinfection from which can become detached contaminate products. Because there a correlation between virulence serotype (and thus phylogenetic division) L. , it important determine if link biofilm formation disease...

ABSTRACT Contamination of soils with toxic metals is a major problem on military, industrial, and mining sites worldwide. Of particular interest to the field bioremediation selection biological markers for end point remediation. In this microcosm study, we focus effect addition mixture (cadmium, cobalt, cesium, strontium as chlorides) soil population structure size ammonia oxidizers that are members beta subgroup Proteobacteria (β-subgroup oxidizers). parallel experiment, were also treated...

When the heart fails, there is often a constellation of biochemical alterations β-adrenergic receptor (βAR) signaling system, leading to loss cardiac inotropic reserve. βAR down-regulation and functional uncoupling are mediated through enhanced activity kinase (βARK1), expression which increased in ischemic failing myocardium. These changes widely viewed as representing an adaptive mechanism, protects against chronic activation. In this study, we demonstrate, using vivo intracoronary...

Background —Genetic manipulation to reverse molecular abnormalities associated with dysfunctional myocardium may provide novel treatment. This study aimed determine the feasibility and functional consequences of in vivo β-adrenergic receptor kinase (βARK1) inhibition a model chronic left ventricular (LV) dysfunction after myocardial infarction (MI). Methods Results —Rabbits underwent ligation circumflex (LCx) marginal artery implantation sonomicrometric crystals. Baseline cardiac physiology...

Although starvation survival has been characterized for many bacteria, few subsurface bacteria have tested, and if any tested in natural porous media. We hypothesized that may be uniquely adapted long-term situ. further conditions (sediment type moisture content) would influence microbial survival. compared capabilities of surface strains Pseudomonas fluorescens a novel Arthrobacter sp. microcosms composed sediments. Bacteria were incubated up to 64 weeks under saturated unsaturated...

A randomized trial of lumbar epidural injections for the treatment acute sciatic pain was carried out. All patients had unilateral sciatica less than six months and never previously been treated in hospital their backs. The treatments used were 20 ml normal saline, 80 mg Depomedrone saline made up to ml, 0.25% bupivacaine solution needling with a standard Touhy injection needle into interspinous ligament but no injection. improved at about same rate all four treatments. Overall, 75% or...

Two Rhodococcus strains which were isolated from a trichloroethylene (TCE)-degrading bacterial mixture and rhodochrous ATCC 21197 mineralized vinyl chloride (VC) TCE. Greater than 99.9% of 1-mg/liter concentration VC was degraded by cell suspensions. [1,2- 14 C]VC suspensions, with the production greater 66% CO 2 20% C-aqueous phase products incorporation 10% C into biomass. Cultures that utilized propane as substrate able to mineralize 28% C]TCE , approximately 40% appearing in another...

A lithoautotrophic, Fe(II) oxidizing, nitrate-reducing bacterium, strain 2002 (ATCC BAA-1479; =DSM 18807), was isolated as part of a study on nitrate-dependent oxidation in freshwater lake sediments. Here we provide an in-depth phenotypic and phylogenetic description the isolate. Strain is gram-negative, non-spore forming, motile, rod-shaped bacterium which tested positive for oxidase, catalase, urease. Analysis complete 16S rRNA gene sequence placed clade within family Neisseriaceae order...

A gram-type positive, motile, coccus-shaped organism was isolated from a radioactive work area. Strain SRS30216T is an orange-pigmented bacterium that catalase-positive, oxidase-negative and urease-negative. The orange pigment most likely carotenoid with absorption peaks at approximately 444, 471 501 nm. Cells normally grew in clusters, but individual, flagellated cells were also observed. Growth of strain occurred temperatures between 11 41 degrees C, pH 5 9 NaCl concentrations up to...

Summary Ninety patients scheduled to undergo minor gynaecological surgery were divided into three groups. Group 1 received propofol only, for both induction and maintenance of anaesthesia. 2 given but inhaled 66% nitrous oxide in addition. 3 had only enflurane thereafter. The incidence nausea group was 0%, 2, 3.4% 3, 9.4%. No patient vomited.

Experimental bioreactors operated as recirculated closed systems were inoculated with bacterial cultures that utilized methane, propane, and tryptone-yeast extract aerobic carbon energy sources degraded trichloroethylene (TCE). Up to 95% removal of TCE was observed after 5 days incubation. Uninoculated inhibited 0.5% Formalin 0.2% sodium azide retained greater than their 20 days. Each bioreactor consisted an expanded-bed column through which the liquid phase a gas recharge allowed direct...

Background Symptomatic cerebral cavernous malformations are a rare sporadic or familial disease, which may cause haemorrhagic strokes epileptic seizures. In 2015, James Lind Alliance Priority Setting Partnership ranked targeted drug therapies as fourth of the top 10 research priorities for malformations. There no disease-modifying drugs malformations, but there several promising candidates with proof concept in human and animal studies. platform trials Objectives (1) Consolidate initiate...

Stable isotope fractionation analysis of an aquifer heavily contaminated with benzene (up to 850 mg L-1) and toluene 50 at a former hydrogenation plant in Zeitz (Saxonia, Germany) has suggested that significant biodegradation was occurring. However, clear evidence been lacking this site. Determining the fate is often determining factor regulatory approval risk-based management strategy. The objective work described here demonstration new tool can be used provide proof or other organics by...

Abstract Thirty closely spaced cores were obtained from Miocene‐aged fluvial, lacustrine and palaeosol subsurface sediments ranging in depth 173 to 197 m at a site south‐central Washington investigate the size composition of microbial community relation sediment geochemical geophysical properties. Total phospholipid fatty acid (PLFA) analysis indicated that greatest concentrations bio‐mass low‐permeability also contained high organic carbon. Community structure, based on lipid analyses situ...

Biofilms play an important role as sorbents in the process of distribution pollutants surface water systems. The sorption properties biofilms will influence kind and amount sorbed substances. heterogeneity provides different sites which exhibit a preference capacity. As dynamic systems, respond physiologically to their environment. Thus, one substance may lead change composition EPS or other biofilm components further alter original properties. In this paper, toluene on was investigated....