A5078248465- CRISPR and Genetic Engineering
- Vector-borne infectious diseases
- RNA and protein synthesis mechanisms
- Evolution and Genetic Dynamics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- Zoonotic diseases and public health
- Viral Infections and Vectors
- Neuroendocrine regulation and behavior
- Bat Biology and Ecology Studies
- Ion channel regulation and function
- RNA regulation and disease
- Insect and Pesticide Research
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- Phosphodiesterase function and regulation
- Signaling Pathways in Disease
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
Massachusetts Institute of Technology
2016-2024
Harvard University
2016-2021
University of Massachusetts Boston
2019
Tufts University
2019
Inspire
2016
If they are able to spread in wild populations, CRISPR-based gene-drive elements would provide new ways address ecological problems by altering the traits of organisms, but potential for uncontrolled tremendously complicates ethical development and use. Here, we detail a self-exhausting form drive system comprising genetic arranged daisy chain such that each drives next. “Daisy-drive” systems can locally duplicate any effect achievable using an equivalent self-propagating system, their...
Mice Against Ticks is a community-guided ecological engineering project that aims to prevent tick-borne disease by using CRISPR-based genome editing heritably immunize the white-footed mice ( Peromyscus leucopus ) responsible for infecting many ticks in eastern North America. Introducing antibody-encoding resistance alleles into local mouse population anticipated disrupt transmission cycle decades. Technology development shaped engagement with community members and visitors islands of...
Abstract RNA-guided gene drive elements could address many ecological problems by altering the traits of wild organisms, but likelihood global spread tremendously complicates ethical development and use. Here we detail a localized form CRISPR-based composed genetic arranged in daisy-chain such that each element drives next. “Daisy drive” systems can duplicate any effect achievable using an equivalent system, their capacity to is limited successive loss non-driving from base chain. Releasing...
White-footed mice are thought to be the most important reservoir host for deer tick-transmitted pathogens that cause Lyme disease and human babesiosis because they primary immature ticks. Transmission would reduced, however, if ticks feed on deer, which not capable of infecting with either pathogen. By directly measuring whether had fed or using a new quantitative PCR (qPCR) assay detect remnants DNA leftover from larval blood meal, we demonstrate utilization by varies significantly over...
Subtropical lone star tick larvae typically emerge in late summer. We found clusters of host-seeking during early June 2018 New York and Massachusetts, USA. Invasion persistence this more northern locations may have been promoted by adaptation to an accelerated life cycle.
Heritable immunization represents a promising approach for controlling infectious diseases by embedding immunity directly into the genomes of wild species that spread human pathogens. Here, we report genetic engineering Mus musculus to produce neutralizing, protective single-chain antibody against Borrelia burgdorferi , causative agent Lyme disease. Engineered mice stably produced LA-2 scFv-albumin fusion protein across multiple generations, demonstrating robust heritability and stability...
CRISPR/Cas9 technology has revolutionized the production of animal models by reducing experimental timelines, slashing costs and streamlining gene editing, leading to a rapid expansion in number unique for study human disease function. However, most non-model animals, many which are important cancer aging research, remain recalcitrant genome engineering due our limited understanding their reproductive biology. Many wild rodents that transmit diseases particularly challenging engineer low...
Abstract CRISPR/Cas9 has revolutionized the field of genome engineering. Yet, as CRISPR toolbox rapidly expanded, there remains a need for comprehensive library reagents that allow users to perform complex cellular and genetic manipulations without requiring labor-intensive generation meet each user’s unique experimental circumstances. Here we described creation validation pNAX consisting 72 different Cas9 gRNA expression plasmids efficient multiplex gene editing, activation, repression in...