Terpenoid synthases constitute a highly diverse gene family producing wide range of cyclic and acyclic molecules consisting isoprene (C5) residues. Often single terpene synthase produces spectrum given chain length, but some can use multiple substrates, products different length. Only few such enzymes has been characterized, the capacity for multiple-substrate be more widespread than previously thought. Here we focused on germacrene A (GAS) that is key cytosolic enzyme in sesquiterpene...

The periplasmic overexpression of recombinant human interferon beta (rhIFN-β)-1b using a synthetic gene in Escherichia coli BL21 (DE3) was optimized shake flasks Response Surface Methodology (RSM) based on the Box-Behnken Design (BBD).This study aimed to predict and develop optimal fermentation conditions for expression rhIFN-β-1b whilst keeping acetate excretion as lowest amount exploit best results condition rhIFN-β bench top bioreactor.The process variables studied were concentration...

Members of the Camelidae (camels, dromedaries, llamas, alpacas, guanacos and vicunas) are known to produce Igs (immunoglobulins) devoid light chains CH1s (constant heavy-chain domains). The antigen-specific binding fragments these antibodies therefore comprise one single domain (the so-called 'VHH') great importance in biotechnological applications. To evaluate expression biological activity sdAbs (single-domain antibodies) plants, which, on account their small size antigen-recognition...

The current study describes multiple-overlap-extension PCR (MOE-PCR) as a simple and effective approach to assembling multiple DNA fragments with various sizes features in single <italic>in vitro</italic> reaction.

Hepatitis E virus (HEV) is a causative agent of acute hepatitis among people different age groups and has high mortality rate up to 30% pregnant women. Therefore, primary prevention HEV infection essential.The aim this study was obtain the highly purified truncated open reading frames 2 (ORF2) protein, which might be future vaccine candidate.The orf2 gene (orf2.1), encoding 112-660 amino acid capsid protein sequence, optimized, synthesized, cloned into pBluescript II SK(+) vector. After...

Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific could be useful finding an effective way for development vaccine. Methods. A form containing amino acids 112-608 used stimulate peripheral blood mononuclear cells (PBMCs) separated HEV-recovered and control groups. Finally, the levels four cytokines, IFN-γ ELISPOT, cell...

A hydroponical experiment was conducted to evaluate the possible interaction of salinity (EC 0 and 9 dSm-1) occurrence exogenous gibberellic acid (GA3) (100 ppm) on imbalance partitioning nutrients in a popular sugarcane genotype (var. CP48-103) its somaclonal tolerant variant. The results revealed that uptake N, K+, Na+ and Cl- content were affected as result salinity, so salt stress induced accumulation toxic elements, namely: CI-, particularly CP48-103. variant performed better by...

Human granulocyte colony-stimulating factor (hG-CSF) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. Transplastomic plants have been emerged a new high potential candidate production recombinant proteins in comparison with transgenic due to extremely level expression, biosafety many other advantages.

Tissue culture technique was used to obtain salt tolerant variants from embryogenic calluses of sugarcane (Saccharum sp. var. CP48-103) that cultured on a selective medium containing different levels NaCl (0, 0.2, 0.4, 0.6, and 0.8% NaCl). A total four plants regenerated the were selected but best them in vigor grown vitro hydroponic systems under salinity stress comparison with source variety. With increasing supply both systems, root growth more adversely affected than shoot growth....

Abstract The production of human interferon alpha2b (IFN-α2b) in two expression systems, tobacco (Nicotiana tabaccum) and Escherichia coli, was compared various aspects such as safety, yield, quality product productivity. In the E. coli system, IFN-α2b expressed under a pelB signal sequence T7lac promoter pET 26b(+) vector. same gene also cloned plant vector (pCAMBIA1304) between cauliflower mosaic virus (CaMV35S) poly A termination region (Nos) transgenic plants. protein both systems...

The demand for recombinant proteins therapeutic or diagnostic use is expanding rapidly in recent years. Human gamma interferon (Hu-IFN-γ) one of these against viruses that it has a high value therapy Therefore used viral infection, cancer and autoimmune diseases. In this study appropriate primers were designed PCR amplification the Hu-IFN-γ gene which was cloned plant expression vector pCAMBIA1304. created construct transferred Agrobacterium tumefaciens strain LBA4404 transformation tomato...

Lettuce (Lactuca sativa L.) is one of the most important vegetable crops. The chloroplast transformation lettuce requires a reproducible method to regenerate shoots directly from leafy explants. direct shoot regeneration in highly depends on genotype and combinations plant growth regulators. Callus induction, average number regenerated per explant percentage shoot-producing explants were studied by investigating effects different concentrations NAA BAP. Orumia American genotypes media...

Although the stem borer Sesamia nonagrioides (Lefèbvre, 1827) is one of most destructive agricultural pests in South and Southwest Iran, structure these populations not yet well understood.In this study, we sequenced two mitochondrial genes (CO1 Cyt-b) collected cultivated sugarcane rice Iran.These genetic data Iranian were compared with those already published from West Palaearctic (Africa Europe).To testing intra-and inter-population variations, different population analyses (e.g.haplotype...

Summary During a survey in Iran, two known species of plant-parasitic nematodes the families Tylenchidae and Criconematidae were reported for first time. The morphological morphometric characters Iranian populations recovered are discussed illustrated based on morphometrics data. population Filenchus orientalis is characterized by having 601-755μm body length, stylet length 9.0-11.3 μm, lateral field with four incisures, tail 100-118 μm males 15-21 long spicules. Hemicriconemoides...

Cyanobacteria have been the focus of extensive researches because their high potential for development new generations useful natural compounds with vast applications. For entire last ten years, a lot attention has dedicated to cyanobacterial lipids as main source valuable materials clean energy production.As there is direct relationship between biofuel properties and compositional characteristics fatty acids, selected lipid-producing strain was examined analyzed in terms acid composition....

C-Phycocyanin (C-PC) from blue-green algae such as Spirulina has been reported to have various pharmacological characteristics, including anti-inflammatory and anti-tumor activities. Recombinant β-subunit of C-PC (C-PC/β) is an inhibitor cell proliferation inducer cancer apoptosis.Since C-PC/β a big potential be used promising prevention or therapy agent, the purpose this study was clone express platensis cpcB gene in bacterial expression system. This significant step for production...