Graphene quantum dots (GQDs), a class of fluorescent carbon materials, have displayed significant potential in various fields such as energy devices, catalysis, sensing, bioimaging, and drug delivery. Because their extremely small size, generally less than 100 nm, they also tremendous plant science research, especially for the delivery nucleic acids, breaking barrier cell walls. In this study, we synthesized GQDs with size range 2–5 characterized them, surface-functionalized them branched...

Obligate root parasitic plants of the Orobanchaceae family exhibit an intricate germination behavior. The host-dependent process these parasites has prompted extensive research into effective control methods. While effect biomaterials such as amino acids and microRNA-encoded peptides have been explored, double-stranded RNAs (dsRNAs) remained unexamined during process. In this study, we asked whether exogenously applied dsRNA can inhibit a parasite, P. aegyptiaca. To end, was designed to...

Accelerating emergence of antimicrobial resistance among food pathogens and consumers' increasing demands for preservative-free foods are two contemporary challenging aspects within the industry. Antimicrobial packaging use natural preservatives promising solutions. In present study, we used beta-casein-one primary self-assembly proteins in milk with a high polymeric film production capability-as fusion partner recombinant expression E 50-52 peptide Escherichia coli. The pET21a-BCN-E...

Sunn pest, Eurygaster integriceps , is a serious pest of cereals in the wide area globe from Near and Middle East to South Europe North Africa. This study described for first time, identification E. trypsin serine protease cathepsin‐L cysteine, transcripts involved digestion, which might serve as targets control management. A total 478 500 base pair long putative cysteine gene sequences were characterized named Tryp Cys, respectively. In addition, tissue‐specific relative expression levels...

Obligate root parasitic plants of the Orobanchaceae family exhibit an intricate germination behavior. The host-dependent process these parasites has prompted extensive research into effective control methods. While effect biomaterials such as amino acids and microRNA-encoded peptides have been explored, double-stranded RNAs (dsRNAs) remained unexamined during process. In this study, we asked whether exogenously applied dsRNA can inhibit a parasite, P. aegyptiaca. To end, was designed to...

Introduction: Wide applications in research, clinical and cosmetic industry of human epidermal growth factor (hEGF) made it a research interest target. Its production different expression systems has shown several limitations. Recombinant hEGF in E. coli is always accompanied by inclusion body formation. The object this study is to the evaluation chromatography-independent approach for EGF E. coli as soluble form. Materials Methods: In order evaluate chromatogram independent purification...

Interferon alpha 2b gene (INF α2b) as a protein with antiviral and antitumor activities is potentially valuable therapeutic to work on. Prior having large-scale production of the target protein, it recommended examine on an experimental scale, so that bacterial host could be proper choice leads us deep insight into subject. In this research, INF α2b sequence was obtained from NCBI data bank, after optimization, subjected cloned expressed in pET28a+. order primary examination Escherichia coli...

Plants offer a promising alternative to microbial fermentation and animal cell cultures for production of recombinant proteins, the major advantages are safety, low cost, post-translation modifications high volume production. t-PA (tissue Plasminogen activator) is trypsin-like serine proteinase superior thrombolytic agent. In this report, cDNA tissue activator was transformed tobacco plants. protein expressed under control of CaMV35S promoter and NOS terminator. A high-expression sequence...

Lettuce (Lactuca sativa L.) is one of the most important vegetable crops. The chloroplast transformation lettuce requires a reproducible method to regenerate shoots directly from leafy explants. direct shoot regeneration in highly depends on genotype and combinations plant growth regulators. Callus induction, average number regenerated per explant percentage shoot-producing explants were studied by investigating effects different concentrations NAA BAP. Orumia American genotypes media...

<title>Abstract</title> Plants are often seen as a potent tool in the recombinant protein production industry. However, unlike bacterial expression, it is not popular method due to low yield and difficulty of extraction purification. Therefore, developing new high efficient easy purify platform crucial. One best approaches make easier utilize Extensin Signal peptide (EXT) translocate outside cell, along with incorporating an Elastin-like polypeptide tag (ELP) enhance purification...