A5035218785- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Radiopharmaceutical Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Medical Imaging Techniques and Applications
- RNA Interference and Gene Delivery
- Exercise and Physiological Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Peptidase Inhibition and Analysis
- SARS-CoV-2 and COVID-19 Research
- Advanced Biosensing Techniques and Applications
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Glaucoma and retinal disorders
- Virus-based gene therapy research
- HER2/EGFR in Cancer Research
- Transgenic Plants and Applications
- Galectins and Cancer Biology
- Analytical Chemistry and Chromatography
- Brucella: diagnosis, epidemiology, treatment
Tarbiat Modares University
2016-2025
Shahid Rajaee Teacher Training University
2021
Islamic Azad University Central Tehran Branch
2011
Kolding Hospital
2011
Razi Vaccine and Serum Research Institute
2007
Tehran University of Medical Sciences
2006
Birjand University of Medical Sciences
2001
Background The programmed cell death protein-1 (PD-1)/programmed receptor ligand 1 (PD-L1) axis critically facilitates cancer cells’ immune evasion. Antibody therapeutics targeting the PD-1/PD-L1 have shown remarkable efficacy in various tumors. Immuno-positron emission tomography (ImmunoPET) imaging of PD-L1 expression may help reshape solid tumors’ immunotherapy landscape. Methods By immunizing an alpaca with recombinant human PD-L1, three clones v ariable domain h eavy chain eavy-chain...
Background Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.
Different approaches have been used to extract DNA from whole blood. In most of these methods enzymes (such as proteinase K and RNAse A) or toxic organic solvents phenol guanidine isothiocyanate) are used. Since expensive, the materials that routinely toxic, it is desirable apply an efficient extraction procedure does not require use such materials. this study, genomic was extracted by salting-out method, but instead using analytical-grade enzyme chemical detergents, normally for isolation,...
Due to the high atomic number of gold nanoparticles (GNPs), they are known as new radiosensitizer agents for enhancing efficiency superficial radiotherapy techniques by increasing dose absorbed in tumor cells wherein can be accumulated selectively. The aim this study was compare effect various common low energy levels orthovoltage x-rays and megavoltage γ-rays (Co-60) on therapeutic HeLa cancer presence conjugated folate non-conjugated (pegylated) GNPs. To achieve this, GNPs with an average...
Background Diagnosis is an important factor in controlling disease. Single-chain fragment variables (scFvs) can be used for diagnosis; however, due to their immobilization issues, application has been limited. Herein, we isolated a SARS-CoV-2 nucleocapsid phosphoprotein (NP)-specific scFv and propose it as diagnostic tool the scFv-displaying phage format overcome issue.
Asymmetric PCR, a simple method to generate single-stranded DNA (ssDNA) aptamers in systematic evaluation of ligand by exponential enrichments rounds, is coupled with limitations. We investigated the essential strategies for optimization conditions perform high-quality asymmetric PCR. Final concentrations primers and template, number PCR cycles, annealing temperature were selected as optimizing variables. The qualities visualized products analyzed ImageJ software. highest proportion...
Members of the Camelidae (camels, dromedaries, llamas, alpacas, guanacos and vicunas) are known to produce Igs (immunoglobulins) devoid light chains CH1s (constant heavy-chain domains). The antigen-specific binding fragments these antibodies therefore comprise one single domain (the so-called 'VHH') great importance in biotechnological applications. To evaluate expression biological activity sdAbs (single-domain antibodies) plants, which, on account their small size antigen-recognition...
Chimeric antigen T cell receptors provide a good approach for adoptive immunotherapy of cancer, especially in the context cancerous cells that fail to express major histocompatibility complex and co-stimulatory molecules. Clinical applications these are limited, mostly due xenogenic origin antibodies, which cause immunogenic reactions. Nanobodies smallest fragments antibodies have great homology human VH low potential. MUC1 is highly attractive immunotherapeutic target owing increased...
Epidermal growth factor receptor variant III (EGFRvIII) is the most common of EGF in many human tumors. This tumor specific and highly immunogenic, thus, it can be used as a target for targeted drug delivery toward cells. The major aim this study was to develop an EGFRvIII-mediated system by anti-EGFRvIII monoclonal antibody (MAb) conjugated doxorubicin (Dox)-loaded nanostructured lipid carriers (NLC) enhance targeting specificity cytotoxic effect Dox on EGFRvIII-overexpressing cell line. In...
Alloimmunization to donor blood group antigens remains a significant problem in transfusion medicine. A proposed method overcome donor−recipient incompatibility is mask the by covalent attachment of poly(ethylene glycol) (PEG) red cell (RBC) membrane. Despite much work development PEG-coating RBCs, there paucity data on optimization technique; it aim this study determine optimum conditions for PEG coating using cyanuric chloride reactive derivative methoxy-PEG as model polymer. Activated...
Recently, the existence of "heavy-chain" antibody in Camelidae has been described. However, as yet there is no data on binding this type to peptides. In addition, was not any report production single-domain antibodies two-humped camels (Camelus bactrianus). present study, these questions are addressed. We showed feasibility immunizing old world camels, cloning repertoire variable domain their heavy-chain antibodies, panning and selection, leading successful identification minimum-sized...
Camelids have a unique immune system capable of producing heavy-chain antibodies lacking the light chains and CH1 (constant domain 1). It has been shown that, in contrast with conventional antibody fragments, variable domains these are functional at or after exposure to high temperatures. In present study, VHH (variable antibody) camel was subcloned into vector Ppiczc expressed Pichia pastoris. ORB1-83 recognizes external mutant EGFR [EGF (epidermal growth factor) receptor], VIII. This...