A5087120963- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Pharmacological Effects of Natural Compounds
- Hepatitis B Virus Studies
- interferon and immune responses
- Cancer therapeutics and mechanisms
- SARS-CoV-2 detection and testing
- Synthesis and biological activity
- Click Chemistry and Applications
- Cancer Research and Treatments
- Andrographolide Research and Applications
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Diverse Scientific Research Studies
- Advanced Biosensing Techniques and Applications
- Bee Products Chemical Analysis
- Plant-based Medicinal Research
- Synthesis and Characterization of Heterocyclic Compounds
- Medicinal Plants and Neuroprotection
- Ginkgo biloba and Cashew Applications
- Essential Oils and Antimicrobial Activity
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Plant Virus Research Studies
- Organophosphorus compounds synthesis
Wannan Medical College
2021-2024
Nankai University
2024
Abstract The outbreak of coronavirus disease 2019 (COVID‐19) pandemic caused by the severe acute respiratory syndrome 2 (SARS‐CoV‐2) is a global threat to human health. In parallel with vaccines, efficacious antivirals are urgently needed. SARS‐CoV‐2 main protease (Mpro) an attractive drug target for antiviral development owing its key roles in virus replication and host immune evasion. Due limitations currently available methods, novel high‐throughput screening assays highest importance...
Recently, geraniin has been identified as a potent antiviral agent targeting SARS-CoV-2 main protease (Mpro). Considering the potential of in COVID-19 treatment, stringent validation for its Mpro inhibition is necessary. Herein, we rigorously evaluated
Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), its genetic and geographical origins remain unclear, resulting in suspicions about natural origin. In one our previous studies, we reported presence a furin cleavage site RRAR junction region between S1 S2 subunits spike protein, which was discovered as first crucial clue for origin tracing SARS-CoV-2. present study, conducted an integrative analysis new genome data from bat Sarbecovirus strains after...
Recently, andrographolide, kaempferol, maslinic acid, rutin, and schaftoside have been identified as potent SARS-CoV-2 main protease (Mpro) inhibitors via molecular docking studies. However, no comprehensive in vitro testing of these compounds against Mpro has conducted. In this study, we rigorously evaluated the inhibition by using combinational experiments, including fluorescence resonance energy transfer (FRET), polarization (FP), dimerization-dependent red fluorescent protein (ddRFP)...
The conventional peptide substrates of SARS-CoV-2 main protease (Mpro) are frequently associated with high cost, unstable kinetics, and multistep synthesis. Hence, there is an urgent need to design affordable stable Mpro for pharmacological research. Herein, we designed a functional substrate based on dimerization-dependent red fluorescent protein (ddRFP) the evaluation inhibitors
IS630/Tc1/mariner (ITm) represents the most widely distributed superfamily of DNA transposons in nature. Currently, bioinformatics research on ITm members primarily involves collecting data existing and emerging organizing them into new groups or families. In present study, our survey revealed that Tc1 IS630 have a broad host range, spanning across all six biological kingdoms (bacteria, fungi, plantae, animalia, archaea protista) viruses. The primary discoveries include first Tn630...
SARS-CoV-2 main protease (Mpro) is responsible for polyprotein cleavage to release non-structural proteins (nsps) viral genomic RNA replication, and its homologues are absent in human cells. Therefore, Mpro has been regarded as one of the ideal drug targets treatment coronavirus disease 2019 (COVID-19). In this study, we first combined fluorescence polarization (FP) technique with biotin-avidin system (BAS) develop a novel sandwich-like FP screening assay quick discovery inhibitors from...
含有不同取代基的（E）-2-（苯并噻唑-2-基）-3-（2-羟基苯基）丙烯腈与亚磷酸二苯酯在水中，无催化剂催化即可发生Michael加环／环化串联反应，可以高产率地生成相应的[2-氨基-3-（苯并噻唑-2-基）-4胁色烯-4-基]膦酸酯衍生物．同时，也尝试了水杨醛、2-（苯并噻唑-2-基）乙腈和亚磷酸二苯酯在Et3N催化下的三组分一锅法反应合成相应的膦酸酯衍生物．