A5031233436- Veterinary Oncology Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Sphingolipid Metabolism and Signaling
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Muscle Physiology and Disorders
- Cardiovascular Conditions and Treatments
- Testicular diseases and treatments
- Wildlife Conservation and Criminology Analyses
- Forensic Entomology and Diptera Studies
- Congenital heart defects research
- Sarcoma Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Head and Neck Anomalies
- Glycosylation and Glycoproteins Research
- Tumors and Oncological Cases
- Gastrointestinal Tumor Research and Treatment
- Tracheal and airway disorders
- Congenital Heart Disease Studies
- Gastrointestinal disorders and treatments
- CRISPR and Genetic Engineering
Azabu University
2014-2025
Ministry of Agriculture, Forestry and Fisheries
2011-2021
Tokyo National Hospital
1998
Polyphenols are abundant in vegetables and fruit. They have been shown to various antitumor, antioxidant, anti-inflammatory effects. Here, we extracted the lipid-soluble fraction of polyphenols from fermented sweet potato (Ipomoea batatas). These mainly contained caffeic acid derivatives with strong antioxidant ability, which hypothesized affect diseases for oxidative stress is a factor, such as cancer. We therefore investigated antitumor chemo-sensitizing effects on E0771 murine breast...
Chicken chaphamaparvovirus causes diarrheal symptoms and can be detected in fecal samples. This study reports the detection of chicken chapparvovirus 2 debilitated chickens with hemorrhagic hepatitis at a broiler farm Japan. After euthanasia necropsy, liver hemorrhage was observed. Nuclear inclusion bodies hepatocytes were identified using histological analysis. High-throughput sequencing analysis RNA from livers three affected revealed infection by anemia virus. Polymerase chain reaction...
This report describes an atypical case of canine acanthomatous ameloblastoma (CAA) with mucinous glandular differentiation in a 12-year-old male Chihuahua right mandibular mass. Initially diagnosed as CAA by biopsy, computed tomography revealed bone lysis, prompting surgical excision. Histological examination showed lobular growth two distinct neoplastic cell populations: typical CAA-like cells and minor structure population. Mucinous is undocumented veterinary literature....
Glisson's sheath is the connective tissue ensheathing portal vein, hepatic artery, and bile duct within liver. Although surrounding liver surface known as capsule, its structure function are poorly understood. In present study, we analyzed mouse, rat, rabbit, feline, canine, monkey, porcine, bovine, equine livers by histochemistry immunohistochemistry analysis of α-smooth muscle actin, keratin 19, podoplanin examined how capsule conserved differs among nine species. tended to thicken...
A 7-year-old mixed-breed cat presented with intermittent dry cough for 2 years. Histological examination of the lung biopsy revealed hyperplasia bronchial and bronchiolar epithelium, intraepithelial infiltration globule leukocytes in many bronchi, bronchioles, terminal bronchioles. Lymphoid aggregates cuffed airways, respiratory alveolar ducts. Terminal airway inflammation was associated type II pneumocyte within adjacent alveoli. Warthin-Starry stain numerous argyrophilic filamentous...
Inflammatory bowel disease (IBD) is a chronic persistent intestinal disorder, with ulcerative colitis and Crohn's being the most common. However, physio-pathological development of IBD still unknown. Therefore, research on etiology treatment has been conducted using variety approaches. Short-chain fatty acids such as 3-hydroxybutyrate (3-HB) are known to have various physiological activities. In particular, production 3-HB by microflora associated suppression inflammatory diseases. this...
Although many host factors important for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been reported, the mechanisms by which virus interacts with cells remain elusive. Here, we identified tripartite motif containing (TRIM) 28, TRIM33, euchromatic histone lysine methyltransferase (EHMT) 1, and EHMT2 as proviral involved in SARS-CoV-2 CRISPR-Cas9 screening. Our result suggested that TRIM28 may play a role viral particle formation EHMT1, be transcription...
Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered virus that found the Americas, Europe and Asia; however, no report of APPV Japan published. We identified an central nervous system Japanese piglets CT firstly determined analysed complete genome sequence. Phylogenetic analysis using nucleotide sequence APPV, named Anna/2020, those APPVs from NCBI database showed were divided into three genotypes (genotypes...
In humans, periostin plays a critical role in the enhancement and chronicity of allergic skin inflammation; however, whether it is involved pathogenesis canine dermatitis remains unknown. The aim this study was to examine expression patterns healthy, atopic, nonatopic chronically inflamed skin. Biopsy specimens from 47 dogs with disease normal tissue 5 adult beagles were examined by light microscopy, immunohistochemistry, situ hybridization. skin, localized just beneath epidermis around hair...
Here we report a pig with amyloid A (AA) amyloidosis associated Streptococcus suis infection and identification of unique sequence in the deposits tissue. Tissues from 180-day-old underdeveloped contained foci necrosis suppurative inflammation S. infection. Congo red stain, immunohistochemistry, electron microscopy revealed intense AA deposition spleen renal glomeruli. Mass spectrometric analysis material extracted showed serum 2 (SAA2) peptide as well previously reported amyloidosis. The...
Abstract Obesity is a global health problem caused by genetic, environmental, and psychological factors associated with various disorders. As such, there growing focus on the prevention of obesity related diseases. The gut microbiota plays crucial role in these diseases has become therapeutic target. Prebiotics, such as poly‐ d ‐3‐hydroxybutyric acid (PHB), have gained attention for their potential to alter microbiota, promote beneficial bacterial growth, alleviate obesity. In this study, we...
In a windowless poultry house raising layer chickens in Kanagawa prefecture, Japan, slight increase the mortality of and decrease egg production were observed. Necropsy revealed numerous tapeworms proglottids chicken intestines. Histopathologically, gut-associated lymphoid tissues observed lamina propria jejunum; however, no significant changes other organs. Numerous hide beetles, Dermestes maculatus DeGeer, intermediate hosts Raillietina cesticillus, house. Following decline beetle numbers,...
Postmortem examination of a meerkat which had been captive for eight years in zoo, revealed multinodular white masses filling the thoracic cavity with systemic metastasis. Microscopically, neoplastic cells were polygonal to spindle-shaped and eosinophilic cytoplasm often variable numbers small lipid droplets. Immunohistochemically, intensely positive vimentin occasionally weakly S-100. Ultrastructurally, several droplets without limiting membranes observed cytoplasm. Based on findings above,...
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in atrophied bursa Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with vaccine IBD virus (IBDV) strain exhibited a highly BF, but not immunosuppression. In this study, administered or wild-type strains IBDV were later vaccinated B1 Newcastle (NDV). Bursal changes examined histologically focus on follicle. The immunoreactivity NDV was also...
An embryonal carcinoma was diagnosed in the abdominal cavity of a 55-day-old male calf. Macroscopically, large volume ascitic fluid observed along with white to yellowish-white masses various sizes densely located on wall and surface organs. There an absence testes scrotum. Histologically, tumor cells were polygonal, mostly composed sheets packed solid patterns occasional papillary tubular structures. Cell nuclei variable size, cellular mitotic rate high. Immunohistochemically, positive for...
We describe a case of human Becker muscular dystrophy (BMD)-like myopathy that was characterized by the declined stainability dystrophin at sarcolemma in pig and immunostaining for on formalin-fixed, paraffin-embedded (FFPE) tissue. The present found meat inspection center. looked appeared healthy ante-mortem inspection. Muscular abnormalities were detected after carcass dressing as pale, discolored skeletal muscles with prominent fat infiltrations considered so-called "fatty dystrophy"....
Background. The slit diaphragm (SD) is a complex of podocyte-specific proteins and plays significant role in glomerular filtration. To understand podocyte biology, it important to determine the expression amount SD proteins. This study aimed quantify absolute nephrin, which believed be major component SD, podocytes apply that method normal puromycin aminonucleoside (PAN) nephrosis rats. Methods. counting for number glomerulus was developed by three-dimensional reconstruction imaging Wilms...
Typing of amyloidosis by mass spectrometry (MS) based proteomic analysis contribute to the diagnosis amyloidosis. For MS analysis, laser microdissection (LMD) is used for amyloid specific sampling. This study aimed establish a method selectively extracting amyloids from formalin-fixed, paraffin-embedded (FFPE) specimens organic solvent instead LMD. The extracts using dimethyl sulfoxide (DMSO), dimethylformamide (DMF), methanol, trifluoroethanol (TFE) or hexafluoro-2-propanol FFPE brain...