A5101666732- Advanced Electron Microscopy Techniques and Applications
- Bacteriophages and microbial interactions
- Characterization and Applications of Magnetic Nanoparticles
- Protein Structure and Dynamics
- Heat shock proteins research
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Microfluidic and Bio-sensing Technologies
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
University of Chinese Academy of Sciences
2024
Center for Excellence in Molecular Cell Science
2024
Chinese Academy of Sciences
2024
Abstract Radial spokes (RS) transmit mechanochemical signals between the central pair (CP) and axonemal dynein arms to coordinate ciliary motility. Atomic-resolution structures of metazoan RS complexes in ependymal cilia, whose rhythmic beating drives circulation cerebrospinal fluid, however, remain obscure. Here, we present near-atomic resolution cryo-EM mouse head-neck complex both monomer dimer forms reveal intrinsic flexibility dimer. We also map genetic mutations related primary...
Abstract In cryo-electron microscopy (cryo-EM), sample preparation, especially for rare or fragile macromolecular assemblies and those suffering from air-water interface denaturation particle orientation distribution problems, is a major bottleneck. Here, we developed characterized an immobilized antibody-based affinity grid (IAAG) strategy based on the high-affinity PA tag/NZ-1 antibody epitope tag system. We used Pyr-NHS as linker to immobilize NZ-1 Fab graphene oxide carbon covered...
As animals evolved from external to internal fertilization, sperm flagella, which once transiently propelled in water reach nearby eggs, developed beat for days or even longer within the female reproductive tract. How flagella were remodeled accordingly remains unclear. Unlike externally fertilizing zebrafish and sea urchin, mammalian feature a unique barrel-shaped bridge between axonemal radial spokes RS1 RS2, though its components function remain unknown. Here, using cryo-EM cryo-ET...