A5101409624- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
- Statistical Methods and Inference
- Heat shock proteins research
- Peptidase Inhibition and Analysis
- Allergic Rhinitis and Sensitization
- Protein Structure and Dynamics
- Statistical Methods and Bayesian Inference
- Viral gastroenteritis research and epidemiology
- Food Allergy and Anaphylaxis Research
- Genetic and Kidney Cyst Diseases
- Cellular transport and secretion
- Cytomegalovirus and herpesvirus research
- Organic Electronics and Photovoltaics
- Adenosine and Purinergic Signaling
- Enterobacteriaceae and Cronobacter Research
University of Chinese Academy of Sciences
2017-2024
Center for Excellence in Molecular Cell Science
2017-2024
Central South University
2024
Chinese Academy of Sciences
2017-2023
Aerospace Information Research Institute
2023
State Key Laboratory of Remote Sensing Science
2023
Zhejiang University
2022
University of Iowa
2020
University of Arkansas for Medical Sciences
2020
Nankai University
2020
Structure of SARS-CoV-2 spike in complex with ACE2 reveals the mechanism induced conformational transitions.
Abstract The proteasome, the primary protease for ubiquitin-dependent proteolysis in eukaryotes, is usually found as a mixture of 30S, 26S, and 20S complexes. These complexes have common catalytic sites, which makes it challenging to determine their distinctive roles intracellular proteolysis. Here, we chemically synthesize panel homogenous ubiquitinated proteins, use them compare 26S proteasomes with respect substrate selection peptide-product generation. We show that can degrade ubiquitin...
Abstract The ongoing pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Neutralizing antibodies against SARS-CoV-2 are an option for drug development treating COVID-19. Here, we report the identification and characterization two groups mouse neutralizing monoclonal (MAbs) targeting receptor-binding domain (RBD) on spike (S) protein. MAbs 2H2 3C1, representing antibody groups, respectively, bind distinct epitopes compatible in...
Abstract The emergence of SARS-CoV-2 Kappa and Beta variants with enhanced transmissibility resistance to neutralizing antibodies has created new challenges for the control ongoing COVID-19 pandemic. Understanding structural nature spike (S) proteins their association ACE2 is significant importance. Here we present two cryo-EM structures each spikes in open open-prone transition states. Compared wild-type (WT) or G614 spikes, variant appear more untwisted/open especially Beta, display a...
Abstract Radial spokes (RS) transmit mechanochemical signals between the central pair (CP) and axonemal dynein arms to coordinate ciliary motility. Atomic-resolution structures of metazoan RS complexes in ependymal cilia, whose rhythmic beating drives circulation cerebrospinal fluid, however, remain obscure. Here, we present near-atomic resolution cryo-EM mouse head-neck complex both monomer dimer forms reveal intrinsic flexibility dimer. We also map genetic mutations related primary...
Abstract The eukaryotic chaperonin TRiC/CCT assists the folding of about 10% cytosolic proteins through an ATP-driven conformational cycle, and essential cytoskeleton protein tubulin is obligate substrate TRiC. Here, we present ensemble cryo-EM structures endogenous human TRiC throughout its ATPase with three them revealing endogenously engaged in different stages. open-state TRiC-tubulin-S1 -S2 maps show extra density corresponding to cis-ring chamber Our structural XL-MS analyses suggest a...
Abstract The recent outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rapid international spread pose a global health emergency. trimeric spike (S) glycoprotein interacts with receptor human ACE2 to mediate viral entry into host-cells. Here we present cryo-EM structures an uncharacterized tightly closed SARS-CoV-2 S-trimer the ACE2-bound-S-trimer at 2.7-Å 3.8-Å-resolution, respectively. inactivated fusion peptide may represent ground prefusion state. binding...
The demand for accurate estimation of aboveground biomass (AGB) at high spatial resolution is increasing in grassland-related research and management, especially those regions with complex topography fragmented landscapes, where grass shrub are interspersed. In this study, based on 519 field AGB observations, integrating Synthetic Aperture Radar (SAR; Sentinel-1) high-resolution (Sentinel-2) remote sensing images, environmental topographical data, we estimated the mountain grassland...
ABSTRACT Beta-propiolactone (BPL) is an inactivating agent that widely used in the vaccine industry. However, its effects on protein antigens and mechanisms of action remain poorly understood. Here we present cryo-electron microscopy (cryo-EM) structures BPL-treated coxsackievirus A16 (CVA16) mature virions procapsids at resolutions 3.9 Å 6.5 Å, respectively. Notably, both particles were found to adopt expanded conformation resembling 135S-like uncoating intermediate, with characteristic...
Abstract Enterovirus D68 (EV-D68) is an emerging pathogen associated with respiratory diseases and/or acute flaccid myelitis. Here, two MAbs, 2H12 and 8F12, raised against EV-D68 virus-like particle (VLP), show distinct preference in binding VLP virion neutralizing different strains. A combination of 8F12 exhibits balanced potent neutralization effects confers broader protection mice than single MAbs when given at onset symptoms. Cryo-EM structures complexed or that both antibodies bind to...
Abstract The proteasome activator PA28αβ affects MHC class I antigen presentation by associating with immunoproteasome core particles (iCPs). However, due to the lack of a mammalian PA28αβ-iCP structure, how regulates remains elusive. Here we present complete architectures and free iCP at near atomic-resolution cryo-EM, determine spatial arrangement between through XL-MS. Our structures reveal slight leaning towards α3-α4 side iCP, disturbing allosteric network gatekeeper α2/3/4 subunits,...
Significance The radial spoke (RS) transmits mechanochemical signals from the central pair (CP) to axonemal dynein arms coordinate ciliary motility. RS head, key CP interaction domain, differs dramatically in morphology between protozoa and metazoa despite conservation of components evolution. In this work, we show that murine head is compositionally distinct Chlamydomonas describe cryo-EM structure its core complex at 3.2-Å resolution. Our results provide a blueprint for understanding not...
It is a time-consuming and costly process to develop affordable high-performance organic photovoltaic materials. Computational methods are essential for accelerating the material discovery by predicting power conversion efficiencies (PCE). In this study, we propose deep learning-based framework (DeepAcceptor) design discover highly efficient small molecule acceptor Specifically, an experimental dataset constructed collecting data from publications. Then, model customized predict PCEs...
Enterobacter cloacae is a well-characterized opportunistic pathogen that closely associated with various nosocomial infections. The O-antigen, which one of the most variable constituents on cell surface, has been used widely and traditionally for serological classification many gram-negative bacteria. E. divided into 30 serotypes, based its O-antigen diversity. In this study, by using genomic comparative-genomic approaches, we analyzed gene clusters 26 serotypes in depth. We also identified...
<h3>Background</h3> CD47 blockade using SIRPα-Fc or anti-CD47 antibodies results in inhibition of the 'do not eat' signal and activation phagocytosis has emerged as a promising cancer treatment strategy. However, targeting leads to various hematological toxicities, particularly anemia thrombocytopenia. Lemzoparlimab (also known TJ011133 TJC4) is fully human, IgG4 antibody that endowed with red blood cell (RBC) sparing property unique binding epitope, potentially differentiating itself from...
Abstract Coxsackievirus A16 (CVA16) causes hand, foot and mouth disease in infants young children. However, no vaccine or anti-viral agent is currently available for CVA16. Here, the functions working mechanisms of two CVA16-specific neutralizing monoclonal antibodies (MAbs), 9B5 8C4, are comprehensively investigated. Both 8C4 display potent neutralization vitro prophylactic therapeutic efficacy a mouse model CVA16 infection. Mechanistically, exerts primarily through inhibiting attachment to...
Missing data arise frequently in clinical and epidemiological fields, particular longitudinal studies. This paper describes the core features of an R package wgeesel, which implements marginal model fitting (i.e., weighted generalized estimating equations, WGEE; doubly robust GEE) for with dropouts under assumption missing at random. More importantly, this comprehensively provide existing information criteria WGEE selection on mean or correlation structures. Also, it can serve as a valuable...
This paper is devoted to the R package JSM which performs joint statistical modeling of survival and longitudinal data. In biomedical studies it has been increasingly common collect both baseline covariates along with a possibly censored time. Instead analyzing outcomes separately, approaches have attracted substantive attention in recent literature shown correct biases from separate enhance information. Most existing adopt linear mixed effects model for component Cox proportional hazards...
The global impact of coronavirus disease 2019 (COVID-19) is unprecedented, and many control prevention measures have been implemented to test for trace COVID-19. However, invisible-spreaders, who are associated with nucleic acid detection asymptomatic infections, received insufficient attention in the current COVID-19 efforts. In this paper, we analyze time series infection data Italy, Germany, Brazil, India Sweden since first wave outbreak address following issues through a experiments. We...
Abstract Radial spokes (RS) transmit mechanochemical signals between the central pair (CP) and axonemal dynein arms to coordinate ciliary motility. However, high-resolution structure of mammalian RS remains missing. Here, we present cryo-EM mouse head-neck complex in both monomer dimer forms reveal intrinsic dynamics dimer. We also map genetic mutations related primary dyskinesia asthenospermia on complex. Moreover, unprecedented cryo-ET sub-tomogram averaging ependymal cilia, whose beating...
Abstract Dermatophagoides farinae , as a common house dust mite species, is one of the main sources allergens in world. At present, found to contain more than 30 groups allergens. These are used for allergen‐specific immunotherapy (AIT) allergic diseases. During AIT process, immunoglobulin G (IgG) antibodies can block E (IgE) antibody‐induced reactions human body. One mechanisms may be that IgG and IgE competitively bind same protein, so it necessary explore binding sites (epitopes) In this...