A5100460216- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Immune responses and vaccinations
- Influenza Virus Research Studies
- RNA regulation and disease
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Particle physics theoretical and experimental studies
- Astrophysics and Cosmic Phenomena
- Monoclonal and Polyclonal Antibodies Research
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Neutrino Physics Research
- T-cell and Retrovirus Studies
- Quantum Chromodynamics and Particle Interactions
Fudan University
2023-2025
Chinese Academy of Sciences
2008-2025
Institute of Zoology
2025
Jingdong (China)
2024
Shandong University
2024
Institute of Optics and Electronics, Chinese Academy of Sciences
2024
Nanchang University
2022-2023
University of Chinese Academy of Sciences
2015-2022
Institut Pasteur of Shanghai
2014-2022
Second Affiliated Hospital of Nanchang University
2022
Structure of SARS-CoV-2 spike in complex with ACE2 reveals the mechanism induced conformational transitions.
Abstract The SARS-CoV-2 Delta variant is currently the dominant circulating strain in world. Uncovering structural basis of enhanced transmission and altered immune sensitivity particularly important. Here we present cryo-EM structures revealing two conformational states spike S/ACE2 complex four states. Our analysis suggests that RBD destabilizations lead to population shift towards more RBD-up S1 destabilized fusion-prone state, beneficial for engagement with ACE2 shedding S1. Noteworthy,...
Abstract The ongoing pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Neutralizing antibodies against SARS-CoV-2 are an option for drug development treating COVID-19. Here, we report the identification and characterization two groups mouse neutralizing monoclonal (MAbs) targeting receptor-binding domain (RBD) on spike (S) protein. MAbs 2H2 3C1, representing antibody groups, respectively, bind distinct epitopes compatible in...
Five pairs of enantiomers based on helical aromatic imides were synthesized, and their absolute configurations determined using X-ray crystal structures. It was found that the not only exhibited large Stokes shifts high quantum yields, but also showed mirror image CD signals full-color CPL properties.
Abstract The emergence of SARS-CoV-2 Kappa and Beta variants with enhanced transmissibility resistance to neutralizing antibodies has created new challenges for the control ongoing COVID-19 pandemic. Understanding structural nature spike (S) proteins their association ACE2 is significant importance. Here we present two cryo-EM structures each spikes in open open-prone transition states. Compared wild-type (WT) or G614 spikes, variant appear more untwisted/open especially Beta, display a...
There is currently no approved antiviral therapy for the prophylaxis or treatment of enterovirus infections, which remain a substantial threat to public health. To discover inhibitors that can be immediately repurposed we developed high-throughput screening assay measures cytopathic effect induced by 71 (EV71) screen an FDA-approved drug library. Itraconazole (ITZ), triazole antifungal agent, was identified as effective inhibitor EV71 replication in low-micromolar range (50% concentrations...
Abstract Massive production of efficacious SARS-CoV-2 vaccines is essential for controlling the ongoing COVID-19 pandemic. We report here preclinical development yeast-produced receptor-binding domain (RBD)-based recombinant protein vaccines. found that monomeric RBD could be efficiently produced as a secreted from transformed Pichia pastoris ( P. ) yeast. Yeast-derived RBD-monomer possessed functional conformation and was able to elicit protective level neutralizing antibodies in mice....
Abstract Background The spike (S) protein of SARS-CoV not only mediates receptor-binding but also induces neutralizing antibodies. We previously identified the domain (RBD) S as a major target antibodies in animal models and thus proposed RBD-based vaccine. However, antigenicity immunogenicity RBD humans need to be characterized. Results Two panels serum samples from recovered SARS patients were included antibody responses against measured by ELISA micro-neutralization assays. found that...
In recent years, enterovirus D68 (EVD68) has been reported increasingly to be associated with severe respiratory tract infections and acute flaccid myelitis (AFM) in children all over the world. Yet, no effective vaccines or antiviral drugs are currently available for EVD68. Although several experimental animal models have developed, immunogenicity protective efficacy of inactivated EVD68 not fully evaluated. To promote development vaccines, we established an Institute Cancer Research (ICR)...
Hand, foot, and mouth disease (HFMD) is an infectious that mainly affects infants children, causing considerable morbidity mortality worldwide. HFMD commonly caused by enterovirus 71 (EV71) coxsackieviruses A16 (CVA16), A6 (CVA6), A10 (CVA10). Formalin-inactivated EV71 vaccines are currently available in China; however, these fail to confer cross-protection against infections other HFMD-causing enteroviruses, highlighting the necessity of developing a multivalent vaccine. Our previous...
Abstract The recent outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rapid international spread pose a global health emergency. trimeric spike (S) glycoprotein interacts with receptor human ACE2 to mediate viral entry into host-cells. Here we present cryo-EM structures an uncharacterized tightly closed SARS-CoV-2 S-trimer the ACE2-bound-S-trimer at 2.7-Å 3.8-Å-resolution, respectively. inactivated fusion peptide may represent ground prefusion state. binding...
Hand, foot and mouth disease (HFMD) is an important public health problem that has emerged over the past several years. HFMD predominantly infects children under seven years old occasionally causes severe in adults. Among enteroviruses, enterovirus 71 (EV71) coxsackievirus 16 (CA16) are major causative agents of HFMD. In addition, adenovirus cocirculates with become a possible additional pathogenic factor for some cases. Here, we have investigated neutralizing antibody responses to both...
ABSTRACT To develop applicable and susceptible models to evaluate the toxicity of nanoparticles, antimicrobial effects CuO nanoparticles (CuO-NPs) on various Saccharomyces cerevisiae ( S. ) strains (wild type, single-gene-deleted mutants, multiple-gene-deleted mutants) were determined compared. Further experiments also conducted analyze mechanisms associated with using copper salt, bulk (bCuO), carbon-shelled (C/Cu-NPs), carbon (C-NPs) for comparisons. The results indicated that growth...
Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated structural basis recognition of EV71 by antibody Four three-dimensional structures particles complex IgG or Fab D5 were reconstructed cryo-electron microscopy (cryo-EM) single particle analysis all at subnanometer...
Hand, foot and mouth disease (HFMD) is a serious public health problem that has emerged over the past several decades. Pathogen detection by Chinese national HFMD surveillance system focused mainly on enterovirus 71 (EV71) coxsackievirus A16 (CA16). Therefore, epidemiological information regarding other causative enteroviruses limited. To identify pandemic in Suzhou, Jiangsu province, China, clinical samples from patients with were collected 2012 to 2013 analyzed. The results revealed CA16...
The recently identified Middle East Respiratory Syndrome Coronavirus (MERS-CoV) causes severe and fatal acute respiratory illness in humans. However, no approved prophylactic therapeutic interventions are currently available. MERS-CoV envelope spike protein serves as a crucial target for neutralizing antibodies vaccine development, it plays critical role mediating viral entry through interactions with the cellular receptor, dipeptidyl peptidase 4 (DPP4). Here, we constructed recombinant rare...
COVID-19 pandemic has severely impacted the public health and social economy worldwide. A safe, effective, affordable vaccine against SARS-CoV-2 infections/diseases is urgently needed. We have been developing a recombinant based on prefusion-stabilized spike trimer of formulated with aluminium hydroxide CpG 7909. The protein was expressed in Chinese hamster ovary (CHO) cells, purified, prepared as stable formulation dual adjuvant. Immunogenicity studies showed that candidate vaccines...
The emergence of multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern threatens the efficacy currently approved vaccines and authorized therapeutic monoclonal antibodies (MAbs). It is hence important to continue searching for SARS-CoV-2 broadly neutralizing MAbs defining their epitopes. Here, we isolate 9 mouse raised against spike protein a prototype strain evaluate potency towards panel variants, including B.1.1.7, B.1.351, B.1.617.1, B.1.617.2. By using...