A5101515517- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Intraocular Surgery and Lenses
- CRISPR and Genetic Engineering
- Ophthalmology and Visual Impairment Studies
- Corneal surgery and disorders
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Connective tissue disorders research
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- Plant-based Medicinal Research
- Bioactive Natural Diterpenoids Research
- Anorectal Disease Treatments and Outcomes
- Salmonella and Campylobacter epidemiology
- Pediatric health and respiratory diseases
- Animal Virus Infections Studies
- Foot and Ankle Surgery
- Bacillus and Francisella bacterial research
- ATP Synthase and ATPases Research
- Urinary and Genital Oncology Studies
- Machine Learning in Bioinformatics
- Cell death mechanisms and regulation
Eye & ENT Hospital of Fudan University
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Fudan University
2024
Tianjin Medical University Cancer Institute and Hospital
2019-2024
Zhengzhou University
2021-2023
Ministry of Education
2023
University of Chinese Academy of Sciences
2020-2022
Institut Pasteur of Shanghai
2020-2022
Lanzhou University
2020-2021
First Hospital of Lanzhou University
2020-2021
Structure of SARS-CoV-2 spike in complex with ACE2 reveals the mechanism induced conformational transitions.
Abstract The ongoing pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Neutralizing antibodies against SARS-CoV-2 are an option for drug development treating COVID-19. Here, we report the identification and characterization two groups mouse neutralizing monoclonal (MAbs) targeting receptor-binding domain (RBD) on spike (S) protein. MAbs 2H2 3C1, representing antibody groups, respectively, bind distinct epitopes compatible in...
Abstract Massive production of efficacious SARS-CoV-2 vaccines is essential for controlling the ongoing COVID-19 pandemic. We report here preclinical development yeast-produced receptor-binding domain (RBD)-based recombinant protein vaccines. found that monomeric RBD could be efficiently produced as a secreted from transformed Pichia pastoris ( P. ) yeast. Yeast-derived RBD-monomer possessed functional conformation and was able to elicit protective level neutralizing antibodies in mice....
Abstract The recent outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rapid international spread pose a global health emergency. trimeric spike (S) glycoprotein interacts with receptor human ACE2 to mediate viral entry into host-cells. Here we present cryo-EM structures an uncharacterized tightly closed SARS-CoV-2 S-trimer the ACE2-bound-S-trimer at 2.7-Å 3.8-Å-resolution, respectively. inactivated fusion peptide may represent ground prefusion state. binding...
Purpose: The purpose of this study was to develop an artificial intelligence (AI)-based intraocular lens (IOLs) power calculation formula for improving the accuracy IOLs calculations in patients with congenital ectopia lentis (CEL). Methods: A total 651 eyes CEL that underwent implantation surgery were included study. An AI-based ensemble formula—the Jiang Formula, developed using a training dataset 520 and evaluated on testing 131 eyes. five-fold cross-validation two-layer learning model...
Pyridoxal 5'-phosphate (PLP) is an essential cofactor for numerous enzymes involved in a diversity of cellular processes living organisms. Previous analysis the Actinobacillus pleuropneumoniae S-8 genome sequence revealed presence pdxS and pdxT genes, which are implicated deoxyxylulose 5-phosphate (DXP)-independent pathway PLP biosynthesis; however, little known about their roles A. pathogenicity. Our data demonstrated that could synthesize by PdxS PdxT enzymes. Disruption genes rendered...
Aims The aim of this study was to analyse the effective lens position (ELP) in patients with Marfan syndrome (MFS) and ectopia lentis (EL). Methods Patients MFS undergoing removal primary intraocular (IOL) implantation were enrolled study. back-calculated ELP obtained vergence formula compared theoretical ELPs. error evaluated among demographic biometric parameters, including axial length (AL), corneal curvature radius (CCR) white-to-white (WTW). Results A total 292 eyes from 200 included....
Antimicrobial peptides are essential to the innate immune defense of mammal against bacterial infection. However, pathogenic bacteria have evolved multiple strategies resist and evade antimicrobial peptides, which is vital survival colonization in hosts. PR-39 a linear porcine peptide containing 39 amino acid residues with high proline content. Resistance has been observed Actinobacillus pleuropneumoniae. little known about factors required for this resistance. In present study, exposure...
Abstract ATP6L, the C subunit of V‐ATPase V0 domain, is involved in regulating acidic tumor micro‐environment and may promote progression. However, expression functional role ATP6L tumors have not yet been well explored. In this study, we found that protein overexpression was related to colorectal cancer histological differentiation ( P < 0.001), presence metastasis 0.001) recurrence = 0.02). liver metastatic foci higher than primary 0.04). notably concomitant with epithelial‐mesenchymal...
Abstract Coxsackievirus A16 (CVA16) causes hand, foot and mouth disease in infants young children. However, no vaccine or anti-viral agent is currently available for CVA16. Here, the functions working mechanisms of two CVA16-specific neutralizing monoclonal antibodies (MAbs), 9B5 8C4, are comprehensively investigated. Both 8C4 display potent neutralization vitro prophylactic therapeutic efficacy a mouse model CVA16 infection. Mechanistically, exerts primarily through inhibiting attachment to...
Introduction Despite prompt and appropriate surgical management, a considerable proportion of patients with congenital ectopia lentis (CEL) suffer from postoperative ametropic amblyopia. To predict identify at-risk early, ensure timely amblyopia treatment, we conducted thorough investigation into the onset progression patterns in CEL. Moreover, an ocular prediction model was constructed for Methods In this prospective cohort study, analysis to reveal prevalence at different time points...
To improve the accuracy of intraocular lens (IOL) power calculation formulas by modifying effective position (ELP) equations for patients with Marfan Syndrome (MFS) and ectopia lentis (EL) undergoing in-the-bag IOL implantation. Eye ENT Hospital Fudan University. Retrospective cohort study. The formula-specific ELP was obtained from SRK/T, T2, Holladay I, HofferQ formulas. back-calculated based on vergence formula using preoperative biometry, postoperative refraction, power. Generalized...
a,b ( a 郑州大学药物研究院 药物关键制备技术教育部重点实验室 郑州 450001) b 省部共建食管癌防治国家重点实验室 450001
Coronary heart disease (CHD) is a common cardiovascular in the elderly, which causes high death rate and low cure rate. Therefore, TCM diagnosis objectification important. This paper proposes Relative Associated Density (RAD) method to analyze data set. RAD results of symptoms syndromes are used performing feature selection, prediction with different classification machines show significant improvements. Compared other traditional selection methods, provides higher Interpretability field.