A5055210544- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Immune responses and vaccinations
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Ferroelectric and Piezoelectric Materials
- Multiferroics and related materials
- Viral-associated cancers and disorders
- COVID-19 epidemiological studies
- Cytomegalovirus and herpesvirus research
- Retinal and Optic Conditions
- Computational Drug Discovery Methods
- NF-κB Signaling Pathways
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Mass Spectrometry Techniques and Applications
- Neuropeptides and Animal Physiology
- Protein purification and stability
- Calcium Carbonate Crystallization and Inhibition
Tsinghua University
2010-2024
Center for Global Health
2020-2021
King Center
2021
Nanjing University of Posts and Telecommunications
2020
Harvard University
2012
A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide to other countries around the world1-3. Here, better understand initial step of infection at atomic level, we determined crystal structure receptor-binding domain (RBD) spike protein SARS-CoV-2 bound cell receptor ACE2. The overall ACE2-binding mode RBD is nearly identical...
Abstract The pandemic caused by emerging coronavirus SARS-CoV-2 presents a serious global public health emergency in urgent need of prophylactic and therapeutic interventions. cellular entry depends on binding between the viral Spike protein receptor-binding domain (RBD) angiotensin converting enzyme 2 (ACE2) target cell receptor. Here, we report isolation characterization 206 RBD-specific monoclonal antibodies (mAbs) derived from single B cells eight infected individuals. These mAbs come...
Soft cellular structures that comprise a solid matrix with square array of holes open avenues for the design novel soft and foldable structures. Our results demonstrate by simply changing shape response porous structure can be easily tuned optimal compaction designed.
Abstract A novel and highly pathogenic coronavirus (2019-nCoV) has caused an outbreak in Wuhan city, Hubei province of China since December 2019, soon spread nationwide spilled over to other countries around the world. To better understand initial step infection at atomic-level, we determined crystal structure 2019-nCoV spike receptor-binding domain (RBD) bound with cell receptor ACE2 2.45 Å resolution. The overall ACE2-binding mode RBD is nearly identical that SARS-CoV RBD, which also...
Abstract Understanding the mechanism for antibody neutralization of SARS-CoV-2 is critical development effective therapeutics and vaccines. We recently isolated a large number monoclonal antibodies from infected individuals. Here we select top three most potent yet variable neutralizing in-depth structural functional analyses. Crystal comparisons reveal differences in angles approach to receptor binding domain (RBD), size buried surface areas, key residues on RBD viral spike glycoprotein....
Abstract Neutralizing antibodies (nAbs) to SARS-CoV-2 hold powerful potentials for clinical interventions against COVID-19 disease. However, their common genetic and biologic features remain elusive. Here we interrogate a total of 165 from eight patients, find that potent nAbs different patients have disproportionally high representation IGHV3-53/3-66 usage, therefore termed as public antibodies. Crystal structural comparison these reveals they share similar angle approach RBD, overlap in...
Abstract In recognizing the host cellular receptor and mediating fusion of virus cell membranes, spike (S) glycoprotein coronaviruses is most critical viral protein for cross-species transmission infection. Here we determined cryo-EM structures spikes from bat (RaTG13) pangolin (PCoV_GX) coronaviruses, which are closely related to SARS-CoV-2. All three receptor-binding domains (RBDs) these two trimers in “down” conformation, indicating they more prone adopt inactive state. However, found...
Significance SARS-CoV-2 continues to evolve through emerging variants, more frequently observed with higher transmissibility. Despite the wide application of vaccines and antibodies, selection pressure on Spike protein may lead further evolution variants that include mutations can evade immune response. To catch up virus’s evolution, we introduced a deep learning approach redesign complementarity-determining regions (CDRs) target multiple virus obtained an antibody broadly neutralizes variants.
As SARS-CoV-2 Omicron and other variants of concern (VOCs) continue spreading worldwide, development antibodies vaccines to confer broad protective activity is a global priority. Here, we report on the identification special group nanobodies from immunized alpaca with potency against diverse VOCs including subvariants BA.1, BA.2 BA.4/5, SARS-CoV-1, major sarbecoviruses. Crystal structure analysis one representative nanobody, 3-2A2-4, discovers highly conserved epitope located between cryptic...
Abstract The omicron variants of SARS-CoV-2 have substantial ability to escape infection- and vaccine-elicited antibody immunity. Here, we investigated the extent such in nine convalescent patients infected with wild-type during first wave pandemic. Among total 476 monoclonal antibodies (mAbs) isolated from peripheral memory B cells, identified seven mAbs broad neutralizing activity all tested, including various subvariants. Biochemical structural analysis indicated majority these bound...
Abstract The α 1A- adrenergic receptor (α 1A AR) belongs to the family of G protein-coupled receptors that respond adrenaline and noradrenaline. AR is involved in smooth muscle contraction cognitive function. Here, we present three cryo-electron microscopy structures human bound endogenous agonist noradrenaline, its selective oxymetazoline, antagonist tamsulosin, with resolutions range from 2.9 Å 3.5 Å. Our active inactive reveal activation mechanism distinct ligand binding modes for...
Bovine horn is composed of a sheath keratin overlying bony core. Previous studies the bovine have focused mainly on its morphology and compositions. In present paper, we performed series uniaxial tension, three-point bending, fracture tests to investigate structural mechanical properties sheaths from subadult cattle, Bos taurus. The effects hydration were examined their variations along longitudinal direction addressed. Scanning electron microscopy surfaces showed that has layered structure...
Infectious disease outbreaks such as the COVID-19 (coronavirus 2019) pandemic call for rapid response and complete screening of suspected community population to identify potential carriers pathogens. Central laboratories rely on time-consuming sample collection methods that are rarely available in resource-limited settings.We present a highly automated fully integrated mobile laboratory fast deployment infectious outbreaks. The was equipped with 6-axis robot arm oropharyngeal swab specimen...
Abstract Epstein-Barr virus (EBV) is associated with a range of epithelial and B cell malignancies as well autoimmune disorders, for which there are still no specific treatments or effective vaccines. Here, we isolate EBV gH/gL-specific antibodies from an EBV-infected individual. One antibody, 1D8, efficiently neutralizes infection two major target types, cells cells. In humanized mice, 1D8 provides protection against high-dose challenge by substantially reducing viral loads tumor burden....
The recently identified Middle East Respiratory Syndrome Coronavirus (MERS-CoV) causes severe and fatal acute respiratory illness in humans. However, no approved prophylactic therapeutic interventions are currently available. MERS-CoV envelope spike protein serves as a crucial target for neutralizing antibodies vaccine development, it plays critical role mediating viral entry through interactions with the cellular receptor, dipeptidyl peptidase 4 (DPP4). Here, we constructed recombinant rare...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs), especially the latest Omicron, have exhibited antibody evasion. Broadly neutralizing antibodies with high potency against Omicron are urgently needed for understanding working mechanisms and developing therapeutic agents. In this study, we characterized previously reported F61, which was isolated from convalescent patients infected prototype SARS-CoV-2, as a broadly all VOCs including BA.1, BA.1.1,...
As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants continue to emerge and spread around the world, antibodies vaccines confer broad potent neutralizing activity are urgently needed. Through isolation characterization of monoclonal (mAbs) from individuals infected with SARS-CoV-2, we identified one antibody, P36-5D2, capable major SARS-CoV-2 concern. Crystal electron cryo-microscopy (cryo-EM) structure analyses revealed that P36-5D2 targeted a conserved epitope on...
Prevention of robust severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in nasal turbinate (NT) requires vivo evaluation IgA neutralizing antibodies. Here, we report the efficacy receptor binding domain (RBD)-specific monomeric B8-mIgA1 and B8-mIgA2, dimeric B8-dIgA1, B8-dIgA2 TH335-dIgA1 against intranasal SARS-CoV-2 challenge Syrian hamsters. These antibodies exhibited comparable neutralization potency authentic virus by competing with human angiotensin converting...
The magnetoelectric (ME) coupling in Terfenol-D/P(VDF-TrFE)/Terfenol-D laminate is investigated as a function of the applied static magnetic field (Hs) and frequency alternating field. A three-dimensional surface chart ME coefficients (αME) versus Hs drawn. landscape highlights three strings resonance peaks an additional peak corresponding to maximum piezomagnetic coefficient d11,m Terfenol-D plate. Based on constitutive relations Terfenol-D, resonances are found be caused by mechanical...
The ongoing evolution and immune escape of SARS-CoV-2, alongside the potential threat SARS-CoV-1 other sarbecoviruses, underscore urgent need for effective strategies against their infection transmission. This study highlights discovery nanobodies from immunized alpacas, which demonstrate exceptionally broad potent neutralizing capabilities recently emerged more divergent SARS-CoV-2 Omicron subvariants including JD.1.1, JN.1, KP.3, KP.3.1.1, as well coronaviruses bats pangolins utilizing...