A5085099457- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Cancer Immunotherapy and Biomarkers
- Coronary Interventions and Diagnostics
- Influenza Virus Research Studies
- Cardiac Imaging and Diagnostics
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Bacterial Infections and Vaccines
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Cardiac Valve Diseases and Treatments
- Immune cells in cancer
- Retinal and Optic Conditions
- Science, Research, and Medicine
- Infection Control and Ventilation
- ECG Monitoring and Analysis
- Immune responses and vaccinations
- Genetics, Aging, and Longevity in Model Organisms
University of Hong Kong
2020-2024
Chinese University of Hong Kong
2021-2024
The University of Adelaide
2023
Zhengzhou University of Light Industry
2022-2023
Beibu Gulf University
2019
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contains the furin cleavage Proline-Arginine-Arginine-Alanine (PRRA) motif in S1/S2 region, which enhances viral pathogenicity but is absent closely related bat and pangolin coronaviruses. Whether bat-like coronaviral variants without PRRA (∆PRRA) can establish natural infections humans unknown. Methods Here, we developed a duplex digital polymerase chain reaction assay to examine ∆PRRA Vero-E6-propagated...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection results in rapid T lymphocytopenia and functional impairment of cells. The underlying mechanism, however, remains incompletely understood. In this study, we focused on characterizing the phenotype kinetics T-cell subsets with mitochondrial dysfunction (MD) by multicolor flow cytometry investigating association between MD functionality. While 73.9% study subjects displayed clinical upon hospital admission, a significant...
Prevention of robust severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in nasal turbinate (NT) requires vivo evaluation IgA neutralizing antibodies. Here, we report the efficacy receptor binding domain (RBD)-specific monomeric B8-mIgA1 and B8-mIgA2, dimeric B8-dIgA1, B8-dIgA2 TH335-dIgA1 against intranasal SARS-CoV-2 challenge Syrian hamsters. These antibodies exhibited comparable neutralization potency authentic virus by competing with human angiotensin converting...
Abstract Background Patients with COVID‐19 display a broad spectrum of manifestations from asymptomatic to life‐threatening disease dysregulated immune responses. Mechanisms underlying the detrimental responses and severity remain elusive. Methods We investigated total 137 APs infected SARS‐CoV‐2. were divided into mild severe patient groups based on their requirement oxygen supplementation. All blood samples collected within three weeks after symptom onset. Freshly isolated PBMCs for B cell...
Accurate extraction of coronary arteries from invasive angiography (ICA) images is essential for the diagnosis and risk stratification artery disease (CAD).In this study, a novel deep learning (DL) method proposed automatically extracting ICA images.A convolutional neural network (CNN) was developed with full-scale skip connections supervisions. The encoder architecture based on residual inception modules to obtain multi-scale features multiple layers different window shapes. Transfer...
MicroRNAs (miRNAs) are vital regulators of non‑small cell lung cancer (NSCLC) development and tumorigenesis. The aim the present study was to explore role miRNA (miR)‑1296 expression in NSCLC. miR‑1296 detected by reverse transcription‑quantitative PCR NSCLC tissues matched normal tissues. association with clinicopathological factors patients evaluated χ2 test. Prognostic value levels assessed using Kaplan‑Meier method a Cox proportional hazards model; Cell Counting Kit‑8, Transwell...
BackgroundMemory CD8+ T cell responses play an essential role in protection against persistent infection. However, HIV-1 evades vaccine-induced memory response by mechanisms that are not fully understood.MethodsWe analyzed the temporal dynamics of recall activity and function during EcoHIV infection a potent PD1-based vaccine immunized immunocompetent mice.FindingsUpon intraperitoneal infection, high levels GAG-specific lymphocytes reduced EcoHIV-infected cells significantly. this protective...
ABSTRACT Robust severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in nasal turbinate (NT) accounts for high viral transmissibility, yet whether neutralizing IgA antibodies can control it remains unknown. Here, we evaluated receptor binding domain (RBD)-specific monomeric B8-mIgA1 and B8-mIgA2, dimeric B8-dIgA1 B8-dIgA2 against intranasal SARS-CoV-2 challenge Syrian hamsters. These exhibited comparably potent neutralization authentic virus by competing with human...
<p>Kinetic changes of CD8+ T subclusters and other subpopulations in response to different immunization approaches</p>
<div>Abstract<p>Memory T cells play a key role in immune protection against cancer. Vaccine-induced tissue-resident memory (T<sub>RM</sub>) the lung have been shown to protect metastasis. Identifying source of T<sub>RM</sub> can help improve strategies, preventing tumor Here, we found that prime-boost vaccination approach using intramuscular DNA vaccine priming, followed by intranasal live-attenuated influenza-vectored (LAIV) boosting induced higher...
<p>Supplementary Figure 8</p>
<p>Characterization of circulating genes across tissues and vaccination groups</p>
<p>Gag mosaic expressing LAIV vaccine construction and characterization</p>
<p>Supplementary Figure 9</p>
<p>Enhanced memory T cell response by systematic prime-nasal booster immunization in lung</p>
<p>Supplementary Figure 6</p>
<p>Enhanced memory T cell response by systematic prime-nasal booster immunization in the spleen</p>
<p>Effect of CXCR3 ligand-receptor interaction on TCM cell recruitment at day 14 post booster immunization</p>