A5051311977- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Lung Cancer Treatments and Mutations
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Long-Term Effects of COVID-19
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Computational Drug Discovery Methods
- Photosynthetic Processes and Mechanisms
- Animal Virus Infections Studies
- Fire effects on ecosystems
- Virus-based gene therapy research
- RNA modifications and cancer
- Infection Control and Ventilation
- Mitochondrial Function and Pathology
- Viral Infections and Outbreaks Research
- Molecular Biology Techniques and Applications
- interferon and immune responses
- PARP inhibition in cancer therapy
- Monoclonal and Polyclonal Antibodies Research
University of Hong Kong
2019-2024
Second Affiliated Hospital of Nantong University
2024
Nantong University
2024
Affiliated Hospital of Nantong University
2024
Beijing University of Chemical Technology
2024
Hospital Universitario Puerta de Hierro Majadahonda
2024
Xiamen University
2017-2018
Illumina (United States)
2016
Roche (United States)
2015
Materials Systems (United States)
2011-2015
Abstract Background A physiological small-animal model that resembles COVID-19 with low mortality is lacking. Methods Molecular docking on the binding between angiotensin-converting enzyme 2 (ACE2) of common laboratory mammals and receptor-binding domain surface spike protein SARS-CoV-2 suggested golden Syrian hamster an option. Virus challenge, contact transmission, passive immunoprophylaxis studies were performed. Serial organ tissues blood harvested for histopathology, viral load titer,...
Abstract Background Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is believed to be mostly transmitted medium- large-sized droplets, although airborne transmission may possible in healthcare settings involving aerosol-generating procedures. Exposure droplets can theoretically reduced surgical mask usage. However, there a lack of experimental evidence supporting usage for prevention COVID-19. Methods We used well-established golden...
The COVID-19 pandemic is the third outbreak this century of a zoonotic disease caused by coronavirus, following emergence severe acute respiratory syndrome (SARS) in 20031 and Middle East (MERS) 20122. Treatment options for coronaviruses are limited. Here we show that clofazimine—an anti-leprosy drug with favourable safety profile3—possesses inhibitory activity against several coronaviruses, can antagonize replication SARS-CoV-2 MERS-CoV range vitro systems. We found molecule, which has been...
Abstract The emergence of novel SARS coronavirus 2 (SARS-CoV-2) in 2019 has triggered an ongoing global pandemic severe pneumonia-like disease designated as (COVID-19). To date, more than 2.1 million confirmed cases and 139,500 deaths have been reported worldwide, there are currently no medical countermeasures available to prevent or treat the disease. As development a vaccine could require at least 12-18 months, typical timeline from hit finding drug registration antiviral is >10 years,...
Abstract The emergence of SARS-CoV-2 variants concern and repeated outbreaks coronavirus epidemics in the past two decades emphasize need for next-generation pan-coronaviral therapeutics. Drugging multi-functional papain-like protease (PLpro) domain viral nsp3 holds promise. However, none known PLpro inhibitors has been shown to be vivo active. Herein, we screened a structurally diverse library 50,080 compounds potential identified noncovalent lead inhibitor F0213 that broad-spectrum...
A cross-lineage therapeutic antibody potently targets the receptor binding site of influenza B virus via multiple mechanisms.
The ongoing Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signals an urgent need for expansion in treatment options. In this study, we investigated the anti-SARS-CoV-2 activities of 22 antiviral agents with known broad-spectrum against coronaviruses and/or other viruses. They were first evaluated our primary screening VeroE6 cells and then most potent further using viral antigen expression, load reduction, plaque reduction...
Effective treatments for coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) are urgently needed. Dexamethasone has been shown to confer survival benefits certain groups of hospitalized patients, but whether glucocorticoids such as dexamethasone and methylprednisolone should be used together with antivirals prevent a boost SARS-CoV-2 replication remains determined. Here, we show the beneficial effect alone in combination remdesivir hamster model...
Host AP2M1 protein is exploited by diverse pathogenic viruses via their YxxØ motif during viral replication cycle.
Coronavirus Disease 2019 (COVID-19) is predominantly a respiratory tract infection that significantly rewires the host metabolism. Here, we monitored cohort of COVID-19 patients' plasma lipidome over disease course and identified triacylglycerol (TG) as dominant lipid class present in severe acute syndrome coronavirus 2 (SARS-CoV-2)-induced metabolic dysregulation. In particular, pinpointed droplet (LD)-formation enzyme diacylglycerol acyltransferase (DGAT) LD stabilizer adipocyte...
Abstract Host survival depends on the elimination of virus and mitigation tissue damage. Herein, we report modulation D-mannose flux rewires virus-triggered immunometabolic response cascade reduces Safe inexpensive can compete with glucose for same transporter hexokinase. Such competitions suppress glycolysis, reduce mitochondrial reactive-oxygen-species succinate-mediated hypoxia-inducible factor-1α, thus virus-induced proinflammatory cytokine production. The combinatorial treatment by...
There are only eight approved small molecule antiviral drugs for treating COVID-19. Among them, four nucleotide analogues (remdesivir, JT001, molnupiravir, and azvudine), while the other protease inhibitors (nirmatrelvir, ensitrelvir, leritrelvir, simnotrelvir-ritonavir). Antiviral resistance, unfavourable drug‒drug interaction, toxicity have been reported in previous studies. Thus there is a dearth of new treatment options SARS-CoV-2. In this work, three-tier cell-based screening was...
KRAS mutation testing is mandatory before prescribing anti-epidermal growth factor monoclonal antibodies in the treatment of advanced colorectal cancer. We describe performance a TaqMelt polymerase chain reaction (PCR) assay-the cobas® Mutation Test-designed to detect 19 mutations codons 12, 13, and 61. The limit detection was determined using DNA blends from cell lines, plasmids, formalin-fixed paraffin-embedded tissue specimens. Assay compared Sanger sequencing panel 188 Discordant...
Viruses exploit the host lipid metabolism machinery to achieve efficient replication.We herein characterize lipids profile reprogramming in vitro and vivo using liquid chromatography-mass spectrometry-based untargeted lipidomics.The lipidome of SARS-CoV-2-infected Caco-2 cells was markedly different from that mock-infected samples, with most changes involving downregulation ceramides.In COVID-19 patients' plasma a total 54 belonging 12 classes were significantly perturbed compared...
ABSTRACT Palivizumab, a humanized murine monoclonal antibody that recognizes antigenic site II on both the prefusion (pre-F) and postfusion (post-F) conformations of respiratory syncytial virus (RSV) F glycoprotein, is only prophylactic agent approved for use treatment RSV infection. However, its relatively low neutralizing potency high cost have limited to restricted population infants at risk severe disease. Previously, we isolated high-potency antibody, 5C4, specifically Ø apex pre-F...
Abstract Background The effect of low environmental temperature on viral shedding and disease severity Coronavirus Disease 2019 (COVID-19) is uncertain. Methods We investigated the virological, clinical, pathological, immunological changes in hamsters housed at room (21°C), (12–15°C), high (30–33°C) after challenge by 105 plaque-forming units severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Results nasal turbinate, trachea, lung load live virus titer were significantly higher...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic. Angiotensin-converting enzyme (ACE2) is an entry receptor for SARS-CoV-2. The full-length membrane form of ACE2 (memACE2) undergoes ectodomain shedding to generate shed soluble (solACE2) that mediates SARS-CoV-2 via receptor-mediated endocytosis. Currently, it not known how the physiological regulation contributes etiology COVID-19 in vivo. present study identifies Membrane-type 1 Matrix...
Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes severe disease in humans case-fatality rates of up to 30%. There are currently very limited treatment options for SFTSV infection. We conducted a drug repurposing program by establishing two-tier test system rapidly screen Food and Drug Administration- (FDA)-approved library compounds anti-SFTSV activity vitro. identified five inhibited replication at low micromolar concentrations,...
Abstract COVID-19 pandemic is the third zoonotic coronavirus (CoV) outbreak of century after severe acute respiratory syndrome (SARS) in 2003 and Middle East (MERS) since 2012. Treatment options for CoVs are largely lacking. Here, we show that clofazimine, an anti-leprosy drug with a favorable safety pharmacokinetics profile, possesses pan-coronaviral inhibitory activity, can antagonize SARS-CoV-2 replication multiple vitro systems, including human embryonic stem cell-derived cardiomyocytes...
Abstract In response to the epidemic and pandemic threats caused by emerging respiratory viral infections, a safe efficient broad‐spectrum antiviral therapy at early onset of infection can significantly improve patients’ outcome. Inhaled dry powder is easy administer delivers agent directly primary site infection, thereby minimizing systemic side effects. Here, spray freeze drying (SFD) technique employed formulate tamibarotene, retinoid derivative with activity, as inhalable powder. The SFD...
Metastatic colorectal cancer continues to have a high fatality rate, with approximately only 14% of patients surviving more than 5 years. To improve the survival rate these patients, development new therapeutic drugs is priority. In this study, we investigated effects Oroxylin A on metastasis human cells and its potential molecular mechanism. This study utilised CCK8 assay, transwell flow cytometry, western blot analysis, docking, HE staining, immunofluorescence xenograft models. The...
The Coronavirus Disease 2019 (COVID-19) pandemic caused by the novel lineage B betacoroanvirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in significant mortality, morbidity, and socioeconomic disruptions worldwide.Effective antivirals are urgently needed for COVID-19.The main protease (M pro ) of SARS-CoV-2 is an attractive antiviral target because its essential role cleavage viral polypeptide.In this study, we performed silico structure-based screening a...