A5010770739- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Neuroendocrine Tumor Research Advances
- Vascular Tumors and Angiosarcomas
- Gastrointestinal Tumor Research and Treatment
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
- Lipid Membrane Structure and Behavior
- Cardiac tumors and thrombi
- Viral Infections and Immunology Research
- Systemic Lupus Erythematosus Research
- vaccines and immunoinformatics approaches
- Neuroblastoma Research and Treatments
- T-cell and B-cell Immunology
- Sarcoma Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Animal Virus Infections Studies
- Pancreatic function and diabetes
- Bioinformatics and Genomic Networks
- interferon and immune responses
- Influenza Virus Research Studies
- Bacteriophages and microbial interactions
- Respiratory viral infections research
University of Hong Kong
2022-2024
The First Affiliated Hospital, Sun Yat-sen University
2024
Sun Yat-sen University
2024
Chinese University of Hong Kong
2022
The in vivo pathogenicity, transmissibility, and fitness of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron (B.1.1.529) variant are not well understood. We compared these virological attributes this new concern (VOC) with those Delta (B.1.617.2) a Syrian hamster model COVID-19. Omicron-infected hamsters lost significantly less body weight exhibited reduced clinical scores, tract viral burdens, cytokine chemokine dysregulation, lung damage than Delta-infected...
ABSTRACT The newly emerging SARS-CoV-2 Omicron (B.1.1.529) variant first identified in South Africa November 2021 is characterized by an unusual number of amino acid mutations its spike that renders existing vaccines and therapeutic monoclonal antibodies dramatically less effective. vivo pathogenicity, transmissibility, fitness this new Variant Concerns are unknown. We investigated these virological attributes the comparison with those currently dominant Delta (B.1.617.2) golden Syrian...
The continual emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VOCs) poses a major challenge to vaccines and antiviral therapeutics due their extensive evasion immunity. Aiming develop potent broad-spectrum anticoronavirus inhibitors, we generated A1-(GGGGS)7-HR2m (A1L35HR2m) by introducing an angiotensin-converting enzyme (ACE2)-derived peptide A1 the N terminus viral HR2-derived HR2m through long flexible linker, which showed significantly...
The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has become more serious because the continuous emergence variants concern (VOC), thus calling for development broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing immunogenicity protein-based subunit vaccines. In this study, we compared effect three adjuvants, including aluminum, nanoparticle manganese and MF59, on COVID-19 vaccine...
Abstract The initial severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) Omicron subvariants, BA.1 and BA.2, are being progressively displaced by BA.5 in many countries. To provide insight on the replacement of BA.2 as dominant SARS‐CoV‐2 variant, we performed a comparative analysis BA.2.12.1 BA.5.2 variants cell culture hamster models. We found that exhibited enhanced replicative kinetics over vitro vivo, which is evidenced viral genome detected at different time points, regardless...
Abstract SARS-CoV-2 nsp3 is essential for viral replication and host responses. The SARS-unique domain (SUD) of exerts its function through binding to proteins RNAs. Herein, we show that SUD highly flexible in solution. intramolecular disulfide bond SARS-CoV absent SUD. Incorporating this allowed crystal structure determination 1.35 Å resolution. However, introducing genome was lethal the virus. Using biolayer interferometry, screened compounds directly identified theaflavin 3,3’-digallate...
Abstract Host survival depends on the elimination of virus and mitigation tissue damage. Herein, we report modulation D-mannose flux rewires virus-triggered immunometabolic response cascade reduces Safe inexpensive can compete with glucose for same transporter hexokinase. Such competitions suppress glycolysis, reduce mitochondrial reactive-oxygen-species succinate-mediated hypoxia-inducible factor-1α, thus virus-induced proinflammatory cytokine production. The combinatorial treatment by...
There are only eight approved small molecule antiviral drugs for treating COVID-19. Among them, four nucleotide analogues (remdesivir, JT001, molnupiravir, and azvudine), while the other protease inhibitors (nirmatrelvir, ensitrelvir, leritrelvir, simnotrelvir-ritonavir). Antiviral resistance, unfavourable drug‒drug interaction, toxicity have been reported in previous studies. Thus there is a dearth of new treatment options SARS-CoV-2. In this work, three-tier cell-based screening was...
Viruses exploit the host lipid metabolism machinery to achieve efficient replication.We herein characterize lipids profile reprogramming in vitro and vivo using liquid chromatography-mass spectrometry-based untargeted lipidomics.The lipidome of SARS-CoV-2-infected Caco-2 cells was markedly different from that mock-infected samples, with most changes involving downregulation ceramides.In COVID-19 patients' plasma a total 54 belonging 12 classes were significantly perturbed compared...
The replication and pathogenicity of SARS-CoV-2 Omicron BA.2 are comparable to that BA.1 in experimental animal models. However, has rapidly emerged overtake become the predominant circulating variant worldwide. Here, we compared fitness cell culture Syrian hamster model COVID-19. Using a reverse genetics approach, found BA.1-specific spike mutation G496S compromises its fitness, which may contribute being outcompeted by real world. Additionally, BA.1-unique substitution confers...
Abstract Genome‐wide association study (GWAS) could identify host genetic factors associated with coronavirus disease 2019 (COVID‐19). The genes or functional DNA elements through which affect COVID‐19 remain uncharted. expression quantitative trait locus (eQTL) provides a path to assess the correlation between variations and gene expression. Here, we firstly annotated GWAS data describe effects, obtaining genome‐wide mapped genes. Subsequently, mechanisms characteristics of were...
Emerging severe acute respiratory syndrome (SARS) coronavirus (CoV)-2 causes a global health disaster and pandemic. Seeking effective anti-pan-CoVs drugs benefit critical illness patients of disease 2019 (COVID-19) but also may play role in emerging CoVs the future.
<h3>Background</h3> Pancreatic angiosarcoma is a rare and highly aggressive tumor originated from lymphatic or vascular endothelial cells with poor prognosis few effective treatments. By analyzing the transcriptomic landscape of pancreatic liver metastasis based on single-cell RNA sequencing, we explored mechanisms leading to its susceptibility invasion metastasis, tapped into potential immunotherapeutic targets for treatment angiosarcoma. <h3>Methods</h3> One patient diagnosed was included...