A5051287584- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Immunotherapy and Immune Responses
- Total Knee Arthroplasty Outcomes
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Orthopaedic implants and arthroplasty
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- vaccines and immunoinformatics approaches
- Aquaculture Nutrition and Growth
- High-Temperature Coating Behaviors
- Inflammatory Biomarkers in Disease Prognosis
- Misinformation and Its Impacts
- HIV Research and Treatment
- Circular RNAs in diseases
- Copper-based nanomaterials and applications
- Machine Fault Diagnosis Techniques
Fudan University
2020-2025
Shanghai Medical College of Fudan University
2021-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Weifang Medical University
2022-2023
Henan University of Science and Technology
2022-2023
China Special Equipment Inspection and Research Institute
2023
Weifang People's Hospital
2022-2023
Hunan University of Science and Technology
2021-2022
Shanghai Public Health Clinical Center
2019-2022
Qingdao University
2022
Abstract The pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has posed serious threats to global health and economy, thus calling for the development safe effective vaccines. receptor-binding domain (RBD) in spike protein SARS-CoV-2 is responsible its binding angiotensin-converting enzyme (ACE2) receptor. It contains multiple dominant neutralizing epitopes serves as an important antigen COVID-19 Here, we showed that immunization mice...
conclusions being considered invalid.
Several potent neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus have been identified. However, antibody-dependent enhancement (ADE) has not comprehensively studied for SARS-CoV-2, and the relationship between enhancing versus activities antibody epitopes remains unknown. Here, we select a convalescent individual with IgG activity characterize his response. Monoclonal isolated from memory B cells target four groups of five non-overlapping...
Abstract The emergence of SARS-CoV-2 variants and potentially other highly pathogenic sarbecoviruses in the future highlights need for pan-sarbecovirus vaccines. Here, we discovered a new STING agonist, CF501, found that CF501-adjuvanted RBD-Fc vaccine (CF501/RBD-Fc) elicited significantly stronger neutralizing antibody (nAb) T cell responses than Alum- cGAMP-adjuvanted mice. Vaccination rabbits rhesus macaques (nonhuman primates, NHPs) with CF501/RBD-Fc exceptionally potent nAb against its...
The emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron subvariants BA.2.86 and JN.1 raise concerns regarding their potential to evade immune surveillance spread globally. Here, we test sera from rhesus macaques immunized with 3 doses of wild-type SARS-CoV-2 receptor-binding domain (RBD)-Fc adjuvanted the STING agonist CF501. We find that can potently neutralize pseudotyped XBB.1.5, XBB.1.16, CH.1.1, EG.5, BA.2.86, JN.1, 50% neutralization titers ranging 3,494 7,424....
CD8+ T cell immune responses are critical for combating infectious diseases and tumours1–3. Antigen cross-presentation, primarily occurring at the endoplasmic reticulum (ER) of dendritic cells, is essential protein-based vaccines to induce responses4. Current efforts have focused on antigen delivery tissue cellular levels, whereas subcellular has been limited facilitating escape from lysosomes into cytosol. In absence a small-sized high-affinity ER-targeting molecule, importance 'last mile'...
Abstract Antibody-dependent enhancement (ADE) has been reported in several virus infections including dengue fever virus, severe acute respiratory syndrome (SARS) and Middle East (MERS) coronavirus infection. To study whether ADE is involved COVID-19 infections, vitro pseudotyped SARS-CoV-2 entry into Raji cells, K562 primary B cells mediated by plasma from recovered patients were employed as models. The of was more commonly detected severely-affected elderly with high titers spike...
The prolonged duration of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has resulted in continuous emergence variants concern (VOC, e.g., Omicron) and interest (VOI, Lambda). These have challenged protective efficacy current COVID-19 vaccines, thus calling for development novel therapeutics against SARS-CoV-2 its VOCs. Here, we constructed a fusion inhibitor-based recombinant protein, denoted as 5-Helix, consisting three heptad repeat 1 (HR1) two 2 (HR2)...
The recently emerged Omicron subvariants XBB and BQ.1.1 have presented striking immune evasion against most monoclonal neutralizing antibodies convalescent plasma. Therefore, it is essential to develop broad-spectrum COVID-19 vaccines combat current future emerging variants. Here, we found that the human IgG Fc-conjugated RBD of original SARS-CoV-2 strain (WA1) plus a novel STING agonist-based adjuvant CF501 (CF501/RBD-Fc) could induce highly potent durable broad-neutralizing antibody (bnAb)...
Several countries have made unremitting efforts to develop an optimal vaccine in the fight against coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2). With increasing occurrence of SARS-CoV-2 variants, current vaccines show decreased neutralizing activities, especially towards Omicron variant. In this context, adding appropriate adjuvants COVID-19 can substantially reduce number required doses and improve efficacy or cross-neutralizing protection....
The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has become more serious because the continuous emergence variants concern (VOC), thus calling for development broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing immunogenicity protein-based subunit vaccines. In this study, we compared effect three adjuvants, including aluminum, nanoparticle manganese and MF59, on COVID-19 vaccine...
Peroxidase-mediated chemokinetic therapy (CDT) can effectively resist bacteria; however, factors such as the high dosage of drugs seriously limit antibacterial effect. Herein, CuFeS2 nanoparticles (NPs) nanozyme system with photothermal effect and peroxidase-like catalytic activity are proposed a combined agent biosafety, high-efficiency, broad-spectrum ability. In addition, as-obtained NPs low doses Cu+ Fe3+ change permeability bacterial cell membranes break antioxidant balance by consuming...
The Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012 and caused continual outbreaks worldwide with high mortality. However, no effective anti-MERS-CoV drug is currently available. Recently, numerous evolutionary studies have suggested that MERS-CoV originated from bat (BatCoV). We herein reported three peptides derived the HR2 region spike protein of BatCoV HKU4, including HKU4-HR2P1, HKU4-HR2P2 HKU4-HR2P3, could bind HR1-derived peptide to form a six-helix bundle...
Abstract New threats posed by the emerging circulating variants of SARS-CoV-2 highlight need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design. Here, we identified a receptor-binding domain (RBD)-binding antibody, XG014, which potently neutralizes β-coronavirus lineage B (β-CoV-B), including SARS-CoV-2, its variants, SARS-CoV bat SARSr-CoV WIV1. Interestingly, antibody family members competing with XG014 binding show reduced levels...
The development of broad-spectrum antivirals against human coronaviruses (HCoVs) is critical to combat the current coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) and its variants, as well future outbreaks emerging CoVs. We have previously identified a polyethylene glycol-conjugated (PEGylated) lipopeptide, EK1C4, with potent pan-CoV fusion inhibitory activity. However, PEG linkers in peptide or protein drugs may reduce stability induce...
The threat to global health caused by three highly pathogenic human coronaviruses (HCoV), SARS-CoV-2, MERS-CoV and SARS-CoV, calls for the development of pan-HCoV therapeutics vaccines. This study reports design engineering a recombinant protein designated HR1LS. It contains linked molecules, each consisting structural domains, including heptad repeat 1 (HR1), central helix (CH), stem (SH) region, in S2 subunit SARS-CoV-2 spike (S) protein. was found that HR1LS automatically formed trimer...
Foot-and-mouth disease virus (FMDV) causes an economically important and highly contagious of cloven-hoofed animals such as cattle, swine, sheep. FMD vaccine is the traditional way to protect against disease, which can greatly reduce its occurrence. However, use vaccines early infection limited. Therefore, alternative strategy applying antiviral agents required control spread FMDV in outbreak situations. As previously reported, LiCl has obviously inhibition effects on a variety viruses...