A5103150435- Metabolomics and Mass Spectrometry Studies
- Traditional Chinese Medicine Analysis
- COVID-19 Clinical Research Studies
- Ginseng Biological Effects and Applications
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Influenza Virus Research Studies
- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Plant-based Medicinal Research
- Viral Infections and Immunology Research
- Peroxisome Proliferator-Activated Receptors
- Antimicrobial Resistance in Staphylococcus
- interferon and immune responses
- Antibiotic Resistance in Bacteria
- Pharmacological Effects of Natural Compounds
- Hydrocarbon exploration and reservoir analysis
- SARS-CoV-2 detection and testing
- Adipose Tissue and Metabolism
- Graphene and Nanomaterials Applications
- Bioactive Natural Diterpenoids Research
- Lipid metabolism and biosynthesis
- Analytical Chemistry and Chromatography
- Research on Leishmaniasis Studies
- Pneumonia and Respiratory Infections
University of Hong Kong
2018-2024
Chinese University of Hong Kong
2023-2024
Hong Kong Science and Technology Parks Corporation
2022
Shanghai Institute of Materia Medica
2014-2021
Chinese Academy of Sciences
2014-2021
Queen Mary Hospital
2018
China Pharmaceutical University
2014-2017
Lipids play numerous indispensable cellular functions and are involved in multiple steps the replication cycle of viruses. Infections by human-pathogenic coronaviruses result diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifestations. Understanding how lipids may modulate pathogenicity remains poor. To this end, we utilized human coronavirus 229E (HCoV-229E) as a model comprehensively characterize host cell lipid response...
Viruses are obligate intracellular microbes that exploit the host metabolic machineries to meet their biosynthetic demands, making these pathways potential therapeutic targets. Here, by exploring a lipid library, we show AM580, retinoid derivative and RAR-α agonist, is highly potent in interrupting life cycle of diverse viruses including Middle East respiratory syndrome coronavirus influenza A virus. Using click chemistry, overexpressed sterol regulatory element binding protein (SREBP) shown...
SARS-CoV-2 has been confirmed in over 450 million cases since 2019. Although several vaccines have certified by the WHO and people are being vaccinated on a global scale, it reported that multiple variants can escape neutralization antibodies, resulting vaccine breakthrough infections. Bacillus Calmette-Guérin (BCG) is known to induce heterologous protection based trained immune responses. Here, we investigated whether BCG-induced immunity protected against K18-hACE2 mouse model. Our data...
Significance The dissemination of antibiotic resistance in pathogenic bacteria is remarkable, and antivirulence agents with novel mechanisms action could lead to innovative therapeutic concepts. simultaneous suppression multiple virulence factors or pathways using small-molecule compounds an attractive approach reducing the Staphylococcus aureus may offer promising potential combating multidrug-resistant such as methicillin-resistant S. . Here we describe a conceptual breakthrough applying...
Coronavirus Disease 2019 (COVID-19) is predominantly a respiratory tract infection that significantly rewires the host metabolism. Here, we monitored cohort of COVID-19 patients' plasma lipidome over disease course and identified triacylglycerol (TG) as dominant lipid class present in severe acute syndrome coronavirus 2 (SARS-CoV-2)-induced metabolic dysregulation. In particular, pinpointed droplet (LD)-formation enzyme diacylglycerol acyltransferase (DGAT) LD stabilizer adipocyte...
Viruses exploit the host lipid metabolism machinery to achieve efficient replication.We herein characterize lipids profile reprogramming in vitro and vivo using liquid chromatography-mass spectrometry-based untargeted lipidomics.The lipidome of SARS-CoV-2-infected Caco-2 cells was markedly different from that mock-infected samples, with most changes involving downregulation ceramides.In COVID-19 patients' plasma a total 54 belonging 12 classes were significantly perturbed compared...
Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the most common causes of hand, foot, mouth disease. Severe EV-A71 CV-A16 infections may be associated with life-threatening complications. However, pathogenic mechanisms underlying these severe clinical pathological features remain incompletely understood. Lipids known to play critical roles in multiple stages virus replication cycle. The specific lipid profile induced upon infection is required for optimal replication....
The emergence of highly transmissible SARS-CoV-2 variants has led to the waves resurgence COVID-19 cases. Effective antivirals against are required. Here we demonstrate that a human-derived peptide 4H30 broad antiviral activity ancestral virus and four Variants Concern (VOCs) in vitro. Mechanistically, can inhibit three distinct steps life cycle. Specifically, blocks viral entry by clustering virions; prevents membrane fusion inhibiting endosomal acidification; inhibits release virions...
Infected macrophages release signaling lipids ( e.g. , linoleic acid) and activate subsequent metabolic pathways to stimulate ROS generation that facilities intracellular bacteria killing, which may represent a novel mechanism of bacterial elimination.
Metabonomics analysis revealed the presumable pathological processes in early stages of AMI and provided useful information on pretreatment efficacy DQTM.
Abstract Venenum Bufonis (VB) is a widely used traditional medicine with serious cardiotoxic effects. The inflammatory response has been studied to clarify the mechanism of cardiotoxicity induced by VB for first time. In present study, Sprague Dawley (SD) rats, were administered (100, 200 and 400 mg/kg) intragastrically, experienced disturbed ECGs (lowered heart rate elevated ST-segment), increased levels serum indicators (creatine kinase (CK), creatine isoenzyme-MB (CK-MB), alanine...
The current COVID-19 pandemic caused by SARS-Cov-2 is responsible for more than 6 million deaths globally. development of broad-spectrum and cost-effective antivirals urgently needed. Medicinal plants are renowned as a complementary approach in which antiviral natural products have been established safe effective drugs. Here, we report that the percolation extract Spatholobus suberectus Dunn (SSP) viral entry inhibitor against SARS-CoV-1/2 other enveloped viruses. inhibitory activities SSP...
The Triassic tectonic evolution and affinity among the Yangtze Block, Qinling Orogenic Belt Songpan-Ganzi Terrane remain subjects of ongoing scientific debate. In this context, sedimentary rocks Xujiahe Formation (second segment) (T 3 x 2 ) represent an ideal case for addressing issue. study, new stratigraphic, geochemical detrital zircon analyses have been conducted on two sections T . stratigraphic assemblage features suggests a braided delta as depositional setting. Whole-rock results...
Host cell lipids play a pivotal role in the pathogenesis of respiratory virus infection. However, direct comparison lipidomic profile influenza and rhinovirus infections is lacking. In this study, we first compared lipid infection bronchial epithelial line. Most features were downregulated for both rhinovirus, especially sphingomyelin features. Pathway analysis showed that sphingolipid metabolism was most perturbed pathway. Functional study bacterial sphingomyelinase suppressed severe acute...
The Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the biggest public health challenge world has witnessed in past decades.SARS-CoV-2 undergoes constant mutations and new variants of concerns (VOCs) with altered transmissibility, virulence, and/or susceptibility to vaccines therapeutics continue emerge.Detailed analysis host factors involved virus replication may help identify novel treatment targets.In this study, we...
Enterovirus A71 (EV-A71) is a common cause of hand, foot, and mouth disease. Severe EV-A71 infections may be associated with life-threatening neurological complications. However, the pathogenic mechanisms underlying these severe clinical pathological features remain incompletely understood. Metabolites are known to play critical roles in multiple stages replication cycles viruses. The metabolic reprogramming induced by viral essential for optimal virus potential antiviral targets. In this...
Fatty acids conjugates (FACs) are ubiquitous but found in trace amounts the natural world. They composed of multiple unknown substructures and side chains. Thus, FACs difficult to be analyzed by traditional mass spectrometric methods. In this study, an integrated strategy was developed global profiling targeted structure annotation complex matrix LTQ Orbitrap. Dicarboxylic acid conjugated bufotoxins (DACBs) Venenum bufonis (VB) were used as model compounds. The new (abbreviated HPNA)...
Antibiotic tolerance poses a threat to current antimicrobial armamentarium. Bacteria at tolerant state survive in the presence of antibiotic treatment and account for persistence, relapse recalcitrance infections. failure may occur due tolerance. Persistent infections are difficult treat often associated with poor prognosis, imposing an enormous burden on healthcare system. Effective strategies targeting antibiotic-tolerant bacteria therefore highly warranted. In this study, small molecule...