A5101731214- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- Pancreatic function and diabetes
- Animal Disease Management and Epidemiology
- Xenotransplantation and immune response
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- T-cell and Retrovirus Studies
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Diabetes and associated disorders
- Organ Transplantation Techniques and Outcomes
- Vaccine Coverage and Hesitancy
- COVID-19 epidemiological studies
- Respiratory viral infections research
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Endoplasmic Reticulum Stress and Disease
University of Hong Kong
2020-2025
Zhongshan Hospital
2021
University of Electronic Science and Technology of China
2021
Chinese University of Hong Kong
2020-2021
South China Agricultural University
2017-2020
Yixing People's Hospital
2020
Jiangsu University
2020
Key Laboratory of Guangdong Province
2020
Tianjin Medical University
2019
Tianjin Tianhe Hospital
2019
The emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. exact origin remains elusive, but presence a distinct motif in S1/S2 junction region suggests possible acquisition cleavage site(s) spike protein that promoted cross-species transmission. Through plaque purification Vero-E6 cultured SARS-CoV-2, we found series variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at junction....
Abstract Current available vaccines for COVID-19 are effective in reducing severe diseases and deaths caused by SARS-CoV-2 infection but less optimal preventing infection. Next-generation which able to induce mucosal immunity the upper respiratory prevent or reduce infections highly transmissible variants of urgently needed. We have developed an intranasal vaccine candidate based on a live attenuated influenza virus (LAIV) with deleted NS1 gene that encodes cell surface expression...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contains the furin cleavage Proline-Arginine-Arginine-Alanine (PRRA) motif in S1/S2 region, which enhances viral pathogenicity but is absent closely related bat and pangolin coronaviruses. Whether bat-like coronaviral variants without PRRA (∆PRRA) can establish natural infections humans unknown. Methods Here, we developed a duplex digital polymerase chain reaction assay to examine ∆PRRA Vero-E6-propagated...
SARS-CoV-2 is of zoonotic origin and contains a PRRA polybasic cleavage motif which considered critical for efficient infection transmission in humans. We previously reported on panel attenuated variants with deletions at the S1/S2 junction spike protein. Here, we characterize pathogenicity, immunogenicity, protective ability further cell-adapted variant, Ca-DelMut, vitro vivo systems. Ca-DelMut replicates more efficiently than wild type or parental virus Vero E6 cells, but causes no...
Classical swine fever virus (CSFV) is a classic Flavivirus that causes the acute, febrile, and highly contagious disease known as classical (CSF). Inflammasomes are molecular platforms trigger maturation of proinflammatory cytokines to engage innate immune defenses induced upon cellular infection or stress. However, relationship between inflammasome CSFV has not been thoroughly characterized. To understand function response infection, we infected porcine peripheral blood monocytes (PBMCs)...
Host adaptive mutations in the influenza A virus (IAV) PB2 protein are critical for human infection, but their molecular action is not well understood. We observe that when IAV containing avian infects mammalian cells, viral ribonucleoprotein (vRNP) aggregates localize to microtubule-organizing center (MTOC) formed. These vRNP resemble LC3B-associated autophagosome structures, with aggresome-like properties, they cause re-distribution of vimentin. However, electron microscopy reveals these...
An intranasal COVID-19 vaccine, DelNS1-based RBD vaccines composed of H1N1 subtype (DelNS1-nCoV-RBD LAIV) was developed to evaluate the safety and immunogenicity in healthy adults. We conducted a phase 1 randomized, double-blinded, placebo-controlled study on participants, age 18-55 naïve, between March September 2021. Participants were enrolled randomly assigned (2:2:1) into low high dose DelNS1-nCoV-RBD LAIV manufactured chicken embryonated eggs or placebo groups. The high-dose vaccine ×...
Classical swine fever (CSF) is an OIE-listed, highly contagious animal disease caused by classical virus (CSFV). The endoplasmic reticulum (ER) organelle in which the replication of many RNA viruses takes place. During viral infection, a series events elicited cells can destroy ER homeostasis that cause stress and induce unfolded protein response (UPR). In this study, we demonstrate was induced during CSFV infection as several UPR-responsive elements such XBP1(s), GRP78 CHOP were...
Abstract Emerging variants of SARS-CoV-2 have been shown to rapidly replace original circulating strains in humans soon after they emerged. There is a lack experimental evidence explain how these natural occurring spread more efficiently than existing transmission. We found that the Alpha variant (B.1.1.7) increased competitive fitness over earlier parental D614G lineages in-vitro and in-vivo systems. Using hamster transmission model, we further demonstrated able replicate shed nasal cavity...
Open AccessCCS ChemistryCOMMUNICATION1 Jan 2022Potential Antiviral Target for SARS-CoV-2: A Key Early Responsive Kinase during Viral Entry Siwen Liu†, Lin Zhu†, Guangshan Xie†, Bobo Wing-Yee Mok, Zhu Yang, Shaofeng Deng, Siu-Ying Lau, Pin Chen, Pui Wang, Honglin Chen and Zongwei Cai Liu† State Laboratory Emerging Infectious Diseases, Department of Microbiology, The University Hong Kong, Pok Fu Lam, Kong SAR 999077 , Zhu† Environmental Biological Analysis, Chemistry, Baptist University,...
Abstract SARS-CoV-2 causes disease varying in severity from asymptomatic infections to severe respiratory distress and death humans. The viral factors which determine transmissibility pathogenicity are not yet clearly characterized. We used the hamster infection model compare replication ability of five strains isolated early cases originating Wuhan, China, February, infected individuals returning Europe elsewhere March 2020. HK-13 HK-95 isolates showed distinct hamsters, with higher virus...
Summary SARS-CoV-2 causes disease varying in severity from asymptomatic infections to severe respiratory distress and death humans. The viral factors which determine transmissibility pathogenicity are not yet clearly characterized. We used the hamster infection model compare replication ability of five strains isolated early cases originating Wuhan, China, February, infected individuals returning Europe elsewhere March 2020. HK-13 HK-95 isolates showed distinct hamsters, with higher virus...
Abstract Background Capsid (C) protein plays an important role in the replication of classical swine fever virus (CSFV). The ubiquitin proteasome system (UPS) involves many viruses via modulation viral proteins. relationship CSFV with UPS is poorly understood and impact 26S on C has never been reported before. Methods In this study, fused EGFP tag expressed PK-15 3D4/2 cells. MG132 3-methyladenine (3-MA) are used to detect roles autophagolysosome expression levels protein. Truncated mutant...
The 26S proteasome, in charge of intracellular protein degradation, plays significant roles the modulation various cellular activities as well interplay between virus and host. However, studies about relationship proteasome classical swine fever (CSFV) is limited up to now. MG132 a inhibitor has been extensively used replication many viruses. Herein, we investigated role CSFV results showed that significantly decreased titers viral RNA copies CSFV-infected PK-15 cells. Further demonstrated...
Abstract SARS-CoV-2 is zoonotic origin and contains a PRRA polybasic cleavage motif which considered critical for efficient infection transmission in humans. We previously reported on panel of attenuated variants with deletion at the S1/S2 junction spike protein. Here we characterize pathogenicity, immunogenicity, protective ability further cell-adapted variant, Ca-DelMut, vitro vivo systems. Ca-DelMut replicates more efficiently than wild type or parental virus cells, but causes no apparent...
Abstract SARS-CoV-2 contains a PRRA polybasic cleavage motif considered critical for efficient infection and transmission in humans. We previously reported that virus variants with spike protein S1/S2 junction deletions spanning this are attenuated. Here we characterize further cell-adapted variant, Ca-DelMut. Ca-DelMut replicates more efficiently than wild type or parental cells, but causes no apparent disease hamsters, despite replicating respiratory tissues. Unlike virus, does not induce...
To investigate whether transplantation of autologous peripheral blood CD34+ stem cells is a viable approach for treating patients with advanced cirrhosis,which currently hindered by shortage in liver donors.A total 100 cirrhosis and who had failed to respond conservative therapy were recruited cells.The success was investigated 6-and 12-months later measuring markers biosynthesis function (coagulation,albumin level,indocyanine green clearance,Child-Pugh score) assessing pathological changes...
Abstract There is a lack of experimental evidence to explain how the B.1.1.7 variant spreads more quickly than pre-existing variants in humans. We found that displays increased competitive fitness over earlier D614G lineages an in-vitro system. Furthermore,, we demonstrated able replicate and shed efficiently nasal cavity other with lower dose shorter duration exposure.