A5013832290- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- Malaria Research and Control
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Protein Structure and Dynamics
- Bacillus and Francisella bacterial research
- Vitamin C and Antioxidants Research
- Computational Drug Discovery Methods
- interferon and immune responses
- Vitamin D Research Studies
- RNA regulation and disease
- ATP Synthase and ATPases Research
- SARS-CoV-2 detection and testing
- CRISPR and Genetic Engineering
- Long-Term Effects of COVID-19
- Virus-based gene therapy research
National University of Singapore
2022-2025
National University Health System
2025
As SARS-CoV-2 Omicron and other variants of concern (VOCs) continue spreading worldwide, development antibodies vaccines to confer broad protective activity is a global priority. Here, we report on the identification special group nanobodies from immunized alpaca with potency against diverse VOCs including subvariants BA.1, BA.2 BA.4/5, SARS-CoV-1, major sarbecoviruses. Crystal structure analysis one representative nanobody, 3-2A2-4, discovers highly conserved epitope located between cryptic...
Abstract The omicron variants of SARS-CoV-2 have substantial ability to escape infection- and vaccine-elicited antibody immunity. Here, we investigated the extent such in nine convalescent patients infected with wild-type during first wave pandemic. Among total 476 monoclonal antibodies (mAbs) isolated from peripheral memory B cells, identified seven mAbs broad neutralizing activity all tested, including various subvariants. Biochemical structural analysis indicated majority these bound...
Striking number of mutations found in the spike protein recently emerged SARS-CoV-2 Omicron subvariants BA.1, BA.2, BA.3 and BA.4/5 has raised serious concerns regarding escape from current antibody therapies vaccine protection. Here, we conducted comprehensive analysis on extent two major lineages BA.1/BA.1.1 BA.2 to neutralization therapeutic antibodies approved by regulatory authorities convalescent plasma patients infected during initial wave pandemic early 2020. We showed that were most...
The non-structural protein (nsp) 14 of coronaviruses plays an important role in maintaining the genomic stability virus during viral replication. This had garnered significant interest towards identification and development inhibitors against nsp14, specifically its exoribonuclease (ExoN) domain. However, no have been successfully developed to date. bioactivity nsp14-ExoN is governed through a complex formation with co-factor nsp10. provides opportunities target assembly as antiviral...
Positive-sense RNA viruses modify intracellular calcium stores, endoplasmic reticulum and Golgi apparatus (Golgi) to generate membranous replication organelles known as viral factories. Viral factories provide a conducive substantial enclave for essential virus via concentrating necessary cellular factors proteins in proximity. Here, we identified the vital role of broad-spectrum antiviral, peruvoside limiting formation Mechanistically, revealed pleiotropic effect Src PLC kinase signaling...
BackgroundRNA viruses account for many human diseases and pandemic events but are often not targetable by traditional therapeutics modalities. Here, we demonstrate that adeno-associated virus (AAV) -delivered CRISPR-Cas13 directly targets eliminates the positive-strand EV-A71 RNA in cells infected mice.MethodsWe developed a Cas13gRNAtor bioinformatics pipeline to design CRISPR guide RNAs (gRNAs) cleave conserved viral sequences across phylogeny an AAV-CRISPR-Cas13 using vitro plaque assay...
The COVID-19 pandemic has brought about unprecedented medical and healthcare challenges worldwide. With the continual emergence spread of new variants, four drug compound libraries were interrogated for their antiviral activities against SARS-CoV-2. Here, we show that screen resulted in 121 promising anti-SARS-CoV-2 compounds, which seven further shortlisted hit validation: citicoline, pravastatin sodium, tenofovir alafenamide, imatinib mesylate, calcitriol, dexlansoprazole, prochlorperazine...
Abstract Background Chikungunya virus (CHIKV) has reemerged as a major public health concern, causing chikungunya fever with increasing cases and neurological complications. Methods In the present study, we investigated low-passage human isolate of East/ Central/South African (ECSA) lineage CHIKV strain LK(EH)CH6708, which exhibited mix small large viral plaques. The plaque variants were isolated designated CHIKV-SP CHIKV-BP, respectively. CHIKV-BP characterized in vitro vivo to compare...
Global cyclical outbreaks of human enterovirus infections has positioned A71 (EV-A71) as a neurotropic virus clinical importance. However, there remains scarcity internationally approved antivirals and vaccines.
The on-going COVID-19 pandemic has given rise to SARS-CoV-2 clades and variants with differing levels of symptoms severity. To this end, we aim systematically elucidate the changes in pathogenesis as evolved from ancestral recent Omicron VOC, on their mechanisms (e.g. cytokine storm) resulting tissue damage, using established K18-hACE2 murine model. We reported that among viruses tested, infection profiles were initially similar between early but started differ greatly starting VOC Delta,...
Abstract As SARS-CoV-2 Omicron and other variants of concern continue spreading around the world, development antibodies vaccines to confer broad protective activity is a global priority. Here, we report on identification special group nanobodies from immunized alpaca with exceptional breadth potency against diverse sarbecoviruses including SARS-CoV-1, BA.1, BA.2. Crystal structure analysis one representative nanobody, 3-2A2-4, revealed highly conserved epitope between cryptic outer face...