A5057298004- Influenza Virus Research Studies
- interferon and immune responses
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
- Virology and Viral Diseases
- Evolution and Genetic Dynamics
- Mycobacterium research and diagnosis
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Pneumocystis jirovecii pneumonia detection and treatment
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Inflammasome and immune disorders
- Tuberculosis Research and Epidemiology
Genome Institute of Singapore
2023-2025
Agency for Science, Technology and Research
2018-2025
Harvard University
2020-2022
Broad Institute
2020
Singapore Immunology Network
2018
Johns Hopkins Medicine
2012
Johns Hopkins University
2012
University of Pittsburgh
2011
The arthropod-transmitted chikungunya virus (CHIKV) causes a flu-like disease that is characterized by incapacitating arthralgia. re-emergence of CHIKV and the continual risk new epidemics have reignited research in pathogenesis. Virus-specific antibodies been shown to control clearance, but present at sub-neutralizing concentrations can also augment infection exacerbates severity. To explore this occurrence, was investigated presence CHIKV-specific both primary human cells murine macrophage...
BackgroundRNA viruses account for many human diseases and pandemic events but are often not targetable by traditional therapeutics modalities. Here, we demonstrate that adeno-associated virus (AAV) -delivered CRISPR-Cas13 directly targets eliminates the positive-strand EV-A71 RNA in cells infected mice.MethodsWe developed a Cas13gRNAtor bioinformatics pipeline to design CRISPR guide RNAs (gRNAs) cleave conserved viral sequences across phylogeny an AAV-CRISPR-Cas13 using vitro plaque assay...
The frequency of individual genetic mutations conferring drug resistance (DR) to Mycobacterium tuberculosis has not been studied previously in Central America, the place origin many immigrants United States. current gold standard for detecting multidrug-resistant (MDR-TB) is phenotypic susceptibility testing (DST), which resource-intensive and slow, leading increased MDR-TB transmission community. We evaluated multiplex allele-specific polymerase chain reaction (MAS-PCR) as a rapid molecular...
Seroprevalence of antibodies against influenza viruses from 1000 people between the ages 0 to 90 years age (100 samples for each decade life) in Pittsburgh, PA, USA was measured. One year removed outbreak novel H1N1 into human population Northern Hemisphere and following emergence a new H3N2 isolate, sera collected determine hemagglutination-inhibition A/H1N1, A/H3N2, B representative vaccine used 2010-2011 season. The seroprevalence virus, A/California/7/2009 (H1N1), increased previously...
While hundreds of genes are induced by type I interferons, their roles in restricting the influenza virus life cycle remain mostly unknown. Using a loss-of-function CRISPR screen cells prestimulated with interferon beta (IFN-β), we identified small number factors required for A replication. In addition to known components signaling pathway, found that replication termination factor 2 (RTF2) restricts at nuclear stage (and perhaps other stages) viral cycle, based on several lines evidence....