A5100633298- Parasitic Infections and Diagnostics
- Virus-based gene therapy research
- Reproductive tract infections research
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- CAR-T cell therapy research
- Amoebic Infections and Treatments
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Data Mining Algorithms and Applications
- Advanced Biosensing Techniques and Applications
- Rabies epidemiology and control
- RNA Research and Splicing
- Animal Disease Management and Epidemiology
- Single-cell and spatial transcriptomics
- Microbial infections and disease research
- Neonatal Respiratory Health Research
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Pediatric health and respiratory diseases
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Plant nutrient uptake and metabolism
- Immune Response and Inflammation
Harbin University of Science and Technology
2025
Chinese University of Hong Kong
2021-2024
Tarim University
2023-2024
Nankai University
2024
Shandong First Medical University
2022
South China Agricultural University
2020-2021
Key Laboratory of Guangdong Province
2021
Taishan Medical University
2015-2019
Henan Agricultural University
2019
Shandong Agricultural University
2011
BackgroundChlamydia psittaci can infect a wide range of avian species, occasionally causing psittacosis (also known as parrot fever) in humans. Most human cases are associated with close contact pet birds or poultry. In December, 2020, an outbreak severe community-acquired pneumonia unknown aetiology was reported hospital Shandong province, China, and some the patients' contacts had respiratory symptoms. Our aims were to determine causative agent this epidemic whether there been...
A newly emerged duck parvovirus, which causes beak atrophy and dwarfism syndrome (BADS) in Cherry Valley ducks, has appeared Northern China since March 2015. To explore the genetic diversity among waterfowl parvovirus isolates, complete genome of an identified isolate designated SDLC01 was sequenced analyzed present study. Genomic sequence analysis showed that shared 90.8%–94.6% nucleotide identity with goose (GPV) isolates 78.6%–81.6% classical Muscovy (MDPV) isolates. Phylogenetic 443...
Abstract AAV-delivered CRISPR/Cas9 (AAV-CRISPR) has shown promising potentials in preclinical models to efficiently insert therapeutic gene sequences somatic tissues. However, the AAV input doses required were prohibitively high and posed serious risk of toxicity. Here, we performed AAV-CRISPR mediated homology-independent knock-in at a new target site mAlb 3’UTR demonstrated that single dose AAVs enabled long-term integration expression hF9 transgene both adult neonatal hemophilia B mice (...
Previously in The Lancet Microbe, Zhenjie Zhang and colleagues1Zhang Z Zhou H Cao et al.Human-to-human transmission of Chlamydia psittaci China, 2020: an epidemiological aetiological investigation.Lancet Microbe. 2022; 3: e512-e520Summary Full Text PDF PubMed Scopus (23) Google Scholar described the outbreak psittacosis China that occurred at end 2020.1Zhang Psittacosis, arising from infection with Gram-negative, obligate intracellular bacterium psittaci, is a global important zoonotic...
Ionic polymer–metal composites (IPMC) have the advantages of a large driving mass ratio, low voltage, and high current sensitivity, but their electrode continuity, energy storage, unclear response mechanisms limit further application development. In this study, Nafion is used as base film metallic silver material to modify IPMC electrodes. The physical electrochemical properties silver-based with three preparation processes are tested, effects different structures on storage performance...
Cryptosporidium parvum is a major zoonotic pathogen responsible for outbreaks of severe diarrhoea in humans and calves. Almost all investigations cryptosporidiosis caused by C. have focused on its IIa subtype family industrialized nations. From December 2018 to April 2019, approximately 200 neonatal calves large cattle farm Hebei Province, China, were diagnosed with watery over 40 died. To investigate the cause outbreak, faecal samples taken during after outbreak from ≤4 weeks age (n = n 56)...
Purpose Chlamydia psittaci ( C. ) has caused sporadic, but recurring, fatal community-acquired pneumonia outbreaks worldwide, posing a serious threat to public health. Our understanding of host inflammatory responses is limited, and many bronchitis cases have rapidly progressed with deterioration. Methods To clarify the response in psittacosis, we analyzed clinical parameters, compared transcriptomic data, concentrations plasma cytokines/chemokines, changes immune cell populations 17...
Cryptosporidium and Enterocytozoon bieneusi are important intestinal pathogens that infect humans various animals. Few reports available regarding the infections of two in Père David's deer. In this study, polymerase chain reaction (PCR) confirmed infection (1.6%) E. 45 (35.2%) 128 fecal samples collected from deer National Nature Reserve Shishou, Hubei Province, China. C. parvum (n = 1) genotype were identified using small subunit rRNA (SSU rRNA) gene. The was further subtyped as IIdA20G1...
To gain new insight into the functional interaction between dendritic cells and methionine encephalin (MENK) combined with pidotimod (PTD), we have analyzed effect of MENK plus PTD on morphology, phenotype functions murine bone-marrow derived (BMDCs) in vitro. The maturation BMDCs cultured presence either or alone, combination PTD, was detected. cell proliferation measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner...
In previous studies, association rules have been proven to be useful in classification problems over high dimensional gene expression data. However, due the nature of such data sets, it is often case that millions can derived many them are covered by exactly same set training tuples and thus support confidence. Ranking selecting from equivalent rule groups remain an interesting unexplored problem. this paper, we look at two interestingness measures for ranking within group: Max-Subrule-Conf...
Paired single-cell and spatially resolved transcriptomics (SRT) data supplement each other, providing in-depth insights into biological processes disease mechanisms. Previous SRT databases have limitations in curating sufficient pairs (SC-SP pairs) real-time heuristic analysis, which hinder the effort to uncover potential insights. Here, we developed Pairpot (http://pairpot.bioxai.cn), a database tailored for paired with analysis. curates 99 high-quality including 1,425,656 spots from 299...
Cryptosporidium parvum is a major zoonotic pathogen responsible for outbreaks of severe diarrhea in humans and calves. Almost all investigations cryptosporidiosis caused by C. have focused on its IIa subtype family industrialized nations. From December 2018 to April 2019, approximately 200 neonatal calves large cattle farm Hebei Province, China presented watery over 40 died. To investigate the cause outbreak, 179 223 fecal specimens were collected during after outbreak from farm, including...
The remarkable advance in gene editing technology presents unparalleled opportunities for transforming medicine and finding cures hereditary diseases. Human trials of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9)-based therapeutics have demonstrated promising results disrupting or deleting target sequences to treat specific However, the potential targeted insertion approaches, which offer distinct advantages over...
BST-2 is a new kind of antivirus protein that induced by IFN α/β. The gene fragment was amplified RT-PCR from pig kidney cells (PK-15) and subcloned into the pLenti7.3/V5-TOPO vector positive clone filtrated. plasmids were transfected HEK293T cells. expressed analyzed SDS-PAGE. Vesicular stomatitis virus (VSV)-CEF restrain experiment Porcine reproductive respiratory syndrome (PRRSV) intervence carried out. Results showed sequence same as in map GenBank its molecular weight 23 kD, which...