A5092173370- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Ovarian function and disorders
- Fish Ecology and Management Studies
- Single-cell and spatial transcriptomics
- Sperm and Testicular Function
Karolinska Institutet
2023-2025
University of Helsinki
2023-2025
Abstract Purpose Ovarian tissue cryopreservation is vital for fertility preservation, yet its effect on ovarian follicle survival and transcriptomic signature requires further investigation. This study delves into the effects of vitrification morphology, function, changes, helping to find possibilities protocol improvements. Methods cortex from 19 bovine animals were used conduct pre- post-vitrification culture followed by histological assessment, immunohistochemistry, TUNEL assay....
Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on level or with limited RNA input. We describe different enzymes for and cDNA amplification, modified lysis buffer, additional clean-up steps before amplification. also detail an optimized sequencing method handpicked single cells, tens hundreds as input material mammalian preimplantation development. For complete details the use execution this protocol, please refer Ezer et al.1
Abstract STUDY QUESTION What is the effect of different oxygen (O 2 ) levels on transcriptomic profile bovine embryos during in vitro culture? SUMMARY ANSWER Embryos grown hypoxia (6% O from zygotes until blastocyst stage had highest formation rate, whereas normoxia (20% delayed reprogramming and embryonic genome activation, induced changes energy metabolism gene expression. WHAT IS KNOWN ALREADY Mammalian preimplantation embryo development a complex sequence events where, within week,...
Abstract Background Embryonic genome activation (EGA) is a critical step in early embryonic development, as it marks the transition from relying on maternal factors to initiation of transcription embryo’s own genome. The associated with EGA are not well understood and need further investigation. PRD-like (PRDL) homeodomain (TFs) considered play crucial roles this event during development but these TFs have evolved differently, even within mammalian lineages. Different numbers PRDL been...