A5072515038- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Endometriosis Research and Treatment
- Effects and risks of endocrine disrupting chemicals
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Ovarian function and disorders
- Extracellular vesicles in disease
- Pregnancy and preeclampsia studies
- Endometrial and Cervical Cancer Treatments
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Estrogen and related hormone effects
- Genomic variations and chromosomal abnormalities
- Birth, Development, and Health
- Reproductive Physiology in Livestock
- Prenatal Screening and Diagnostics
- Nerve injury and regeneration
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Cancer Risks and Factors
- Oral Health Pathology and Treatment
- Cutaneous lymphoproliferative disorders research
- Neuropeptides and Animal Physiology
- Genetic Syndromes and Imprinting
Tallinn University of Technology
2014-2025
Competence Centre on Health Technologies (Estonia)
2011-2020
University of Tartu
2009-2015
University of Helsinki
2007
Cytokines are key modulators of the immune system and also contribute to regulation ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used analyze follicular cytokines (proinflammatory: IL-1 β , IL-6, IL-18, IFN- γ α TNF- IL-12, IL-23;, anti-inflammatory: G-CSF), chemokines (MIP-1 MIP-1 MCP-1, RANTES, IL-8), other biomarkers (sAPO-1/Fas, CD44(v6)) in 153 women undergoing vitro fertilization (IVF). Cytokine origin studied by mRNA analysis granulosa cells. Higher...
Does cellular composition of the endometrial biopsy affect gene expression profile whole-tissue samples?The differences in epithelial and stromal cell proportions biopsies modify profiles results differential analyses.Each type has its unique profile. The cells vary tissue during menstrual cycle, along with individual technical variation due to method tools used obtain biopsy.Using cell-population specific transcriptome data computational deconvolution approach, we estimated taken early...
The period of time when the embryo and endometrium undergo significant morphological alterations to facilitate a successful implantation-known as "window implantation"-is critical moment in human reproduction. Embryo communicate extensively during this period, lipid bilayer bound nanoscale extracellular vesicles (EVs) are purported be integral communication.To investigate nature EV-mediated embryo-maternal communication, we have supplemented trophoblast analogue spheroid (JAr) derived EVs an...
Women of reproductive age are exposed to ubiquitous chemicals such as phthalates, parabens, and per- polyfluoroalkyl substances (PFAS), which have potential endocrine disrupting properties might affect fertility. Our objective was investigate associations between endocrine-disrupting (EDCs) female fertility in two cohorts women attending clinics. In a total population 333 Sweden Estonia, we studied the fertility, evaluating ovarian sensitivity index (OSI) an indicator response, well clinical...
The plasticizer di(2-ethylhexyl) phthalate (DEHP) is known to have endocrine-disrupting properties mediated by its many metabolites that form upon exposure in biological systems. In a previous study, we reported an inverse association between DEHP the human ovarian follicular fluid (FF) and responsiveness of follicles controlled stimulation during vitro fertilization (IVF) treatments. Here, explored this further through molecular analysis FF samples. Ninety-six IVF patients from Swedish (N =...
Communication between various ovarian cell types is a prerequisite for folliculogenesis and ovulation. In antral follicles granulosa cells divide into two distinct populations of mural cumulus (CGC), enveloping the antrum surrounding oocyte, respectively. Both types, with compartment in excess, contribute to floating (FGC) population follicular fluid. The aim this study was compare transcriptomes FGC CGC stimulated obtained from 19 women undergoing IVF–ICSI procedure. were fluid during...
The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signaling and are involved processes of folliculogenesis oocyte maturation. Studies on gene expression regulation human interest due their potential for estimating viability vitro fertilization success. However, posttranscriptional studies micro-RNA (miRNA) level ovary have been scarce. current study determined miRNA profile by deep sequencing 2 intrafollicular somatic cell types: mural cumulus (MGCs CGCs,...
Accumulating data have shown the involvement of microRNAs (miRNAs) in endometriosis pathogenesis. In this study, we used a novel approach to determine endometriotic lesion-specific miRNAs by high-throughput small RNA sequencing paired samples peritoneal lesions and matched healthy surrounding tissues together with eutopic endometria same patients. We found five specific epithelial cells – miR-34c, miR-449a, miR-200a, miR-200b miR-141 showing significantly higher expression compared tissues....
Abstract The inner uterine lining (endometrium) is a unique tissue going through remarkable changes each menstrual cycle. Endometrium has its characteristic DNA methylation profile, although not much known about the endometrial methylome throughout impact of on gene expression and thereby function tissue, including establishing receptivity to implanting embryo, also unclear. Therefore, this study used genome-wide technologies characterize correlation between in biopsies collected from 17...
Abstract Context Clinically used endometrial (EM) receptivity assays are based on transcriptomic patterning of biopsies at midsecretory endometrium (MSE) to identify the possible displacement or disruption window implantation (WOI) in patients with recurrent failure (RIF). However, invasive and cannot be performed same cycle vitro fertilization embryo transfer, while uterine fluid (UF) analysis is considered minimally can immediately precede transfer. Objective To determine whether UF...
A significant proportion of mammalian fertilization is mediated through the proteomic composition sperm surface. These protein constituents can present as biomarkers to control and regulate breeding agricultural animals. Previous studies have addressed bovine cell apical plasma membrane (PM) proteome with nitrogen cavitation enrichment. Alternative workflows would enable expand compositional data more globally around entire sperm's We used a surface biotin-labeling in combination...
The endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about miRNAs involved mid-secretory functions. We analysed complete miRNome from early secretory (pre-receptive) (receptive) phases fertile women patients with recurrent failure (RIF) reveal differentially expressed (DE) endometrium. Furthermore, we investigated...
Cell-free RNAs have the potential to act as a means of gene expression regulation between cells and are therefore used diagnostic markers describing state tissue environment. The origin functions such in human ovarian follicle, environment oocyte maturation, unclear. current study investigates difference microRNA profiles fertile women polycystic ovary syndrome (PCOS) patients three compartments from same preovulatory follicle: mural granulosa (MGC), cell-free follicular fluid (FF),...
Abstract Purpose Ovarian tissue cryopreservation is vital for fertility preservation, yet its effect on ovarian follicle survival and transcriptomic signature requires further investigation. This study delves into the effects of vitrification morphology, function, changes, helping to find possibilities protocol improvements. Methods cortex from 19 bovine animals were used conduct pre- post-vitrification culture followed by histological assessment, immunohistochemistry, TUNEL assay....
Follicular fluid extracellular vesicles (FF EVs) facilitate communication between oocytes and somatic cells within the ovarian follicle, playing a pivotal role in follicular development. This study highlights molecular functional distinctions small (SEV) large (LEV) FF EV subpopulations, revealing their specialized regulatory roles granulosa cell (GC) biology consequential impact on function. Single-EV profiling uncovered distinct tetraspanin distributions, with LEVs containing lower...
STUDY QUESTION: How do human cortical (cPOCs) and medullary (mPOCs) primary ovarian cells respond to acute X-ray exposure? SUMMARY ANSWER: Acute high-dose exposure causes a shift in cPOCs mPOCs transcriptomic profiles impairs significantly their cell-cell adhesion ability. WHAT IS KNOWN ALREADY: Radiotherapy is leading cancer treatment, due its effectiveness targeting malignant cells. However, it can also affect healthy cells, potentially causing organ dysfunction, among which ovaries. When...
Targeted next-generation sequencing (NGS) methods have become essential in medical research and diagnostics. In addition to NGS sensitivity high-throughput capacity, precise biomolecule counting based on unique molecular identifier (UMI) has potential increase detection accuracy. Although UMIs are widely used basic its introduction clinical assays is still progress. Here, we present a robust cost-effective TAC-seq (Targeted Allele Counting by sequencing) method that uses estimate the...
While follicular fluid (FF) is well known to provide an optimal environment for oogenesis, its functional roles following release into the oviduct during ovulation are currently elusive. We hypothesized that FF and FF-derived extracellular vesicles (EVs) may be conveyors of signals capable inducing functionally-relevant transcriptional responses in oviductal cells. The aim this study was, therefore, evaluate effect EVs on transcriptome primary bovine epithelial cells (BOECs). examined gene...
Chemical health risk assessment is based on single chemicals, but humans and wildlife are exposed to extensive mixtures of industrial substances pharmaceuticals. Such exposures life-long correlate with multiple morbidities, including infertility. How combinatorial effects chemicals should be handled in hazard characterization open questions. Further, test systems missing for several relevant outcomes reproductive fertility women. Here, our aim was screen ovarian cell models phthalate induced...
Phthalates are endocrine disrupting chemicals (EDCs) found in common consumer products such as soft plastics and cosmetics. Although the knowledge regarding adverse effects of phthalates on female fertility accumulating, information hormone sensitive endometrium is still scarce. Here, we studied endometrial cell proliferation gene expression. Human primary epithelial stromal cells were isolated from healthy fertile-aged women (n=3), compared to lines T-HESC Ishikawa. Three different...