A5043171946- Parasites and Host Interactions
- Sarcoma Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Parasite Biology and Host Interactions
- Research on Leishmaniasis Studies
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Zoonotic diseases and public health
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Wnt/β-catenin signaling in development and cancer
- Inflammatory mediators and NSAID effects
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Poxvirus research and outbreaks
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Vector-borne infectious diseases
- Immunotherapy and Immune Responses
- Eicosanoids and Hypertension Pharmacology
University of Colorado Anschutz Medical Campus
2018-2025
University of Colorado Denver
2013-2024
University of Pennsylvania
1987-1990
Houghton University
1977
Eicosanoids are bioactive lipid mediators derived from arachidonic acid1 (AA), which is released by cytosolic phospholipase A2 (cPLA2). AA metabolized through three major pathways, cyclooxygenase (COX), lipoxygenase (LO) and cytochrome P450, to produce a family of eicosanoids, individually have been shown pro- or anti-tumorigenic activities in cancer. However, cancer progression likely depends on complex changes multiple eicosanoids produced cells tumor microenvironment systematic...
Abstract Purpose: Ewing sarcoma (EwS) is the second most common bone cancer in children, accounting for 2% of pediatric diagnoses. Patients who present with metastatic disease at time diagnosis have a dismal prognosis, compared to >70% 5-year survival those localized disease. Novel therapeutic approaches that can impact are desperately needed, as well deeper understanding heterogeneity EwS tumors. Experimental Design: Here, we utilized single cell RNA-sequencing characterize...
Abstract Ewing sarcoma is the second most common bone cancer in children, accounting for 2% of pediatric diagnoses. Patients who present with metastatic disease at time diagnosis have a dismal prognosis, compared to >70% 5-year survival those localized disease. Here, we utilized single cell RNA-sequencing characterize transcriptional landscape primary tumors and surrounding tumor microenvironment (TME). Copy-number analysis identified subclonal evolution within patients prior treatment....
Lung-specific overexpression of prostacyclin synthase (PGIS) decreases tumor initiation in murine lung cancer models. Prostacyclin analogs prevent formation mice and reverse bronchial dysplasia former smokers. However, the effect on progression has not been well studied. We investigated effects pulmonary PGIS an orthotopic immunocompetent mouse model using two cell lines. Pulmonary significantly inhibited CMT167 growth, increased CXCL9 expression, CD4+ tumor-infiltrating lymphocytes....
SUMMARY The expression of virus, thymus and tumour-specific antigens was studied in two Marek’s disease lymphoblastoid cell lines (MSB-1 HPRS-line 2). proportions cells which spontaneously expressed virus or formed infective centres vitro were considerably higher MSB-1 than 2, but did not exceed 10%. In contrast to antigens, thymus-specific on the majority cells. Treatment with IdUrd increased proportion forming both expressing MSB-1. A decline IdUrd-treated untreated cultures noted during...
SUMMARY Precipitating antigens present in extracts of chick embryo cells infected with the HPRS-16 attenuated strain Marek’s disease virus (att-MDV) were separated by gel filtration on Sephadex G200 and some their properties determined. The two main detected convalescent MD serum, referred to as ‘B’ ‘C’ antigens, had mobilities 0.55 0.25 respectively relative phenol red electrophoresis 7.5% acrylamide gel. B antigen was relatively stable low mol. wt. comparison C antigen. instances also...
Ewing sarcoma is the second most common bone cancer in children, and while patients who present with metastatic disease at time of diagnosis have a dismal prognosis. tumors are driven by fusion gene EWS/Fli1, these genetically homogenous, transcriptional heterogeneity can lead to variety cellular processes including metastasis. In this study, we demonstrate that cells, canonical Wnt/β-Catenin signaling pathway heterogeneously activated vitro vivo, correlating hypoxia EWS/Fli1 activity. cells...
Abstract These studies assess the roles of subpopulations T lymphocytes in inducing and modulating resistance to Schistosoma mansoni. CDF rats were depleted RT 7.1+ (anti-Pan-T), W3/25+ (anti-T helper/inducer), or OX8+ suppressor) cells by vivo administration monoclonal antibodies (mAb). The development parasites immunity challenge S. mansoni compared with results undepleted normal congenitally athymic rats. Discrete adoptively transferred ascertain effects upon parasite protective immune...
These studies explore auto-anti-idiotypic mechanisms as potential regulators of the protective immune response against Schistosoma mansoni. Anti-idiotypic responses were stimulated by immunization mice with lymphoblasts, bearing specific idiotypic receptors. receptors produced in vitro stimulation Ag-reactive T cells soluble cercarial immunogen, keyhole limpet hemocyanin, or Con A. The animals then exposed to irradiated cercariae, SRBC. results indicate that immunogen lymphoblast recipient...
Auto-anti-idiotypic mechanisms can regulate the protective immune response against Schistosoma mansoni. Anti-idiotypic responses were stimulated by immunization of mice either with nonspecifically induced lymphoblasts, produced Con A, or Ag-induced lymphoblasts bearing specific idiotypic receptors. The effect anti-idiotypic upon clonotypic cellular reactivity was assessed in vitro through suppression antigen-mediated blast transformation cloned T cells and vivo resistance to S. mansoni...
These studies explore the suppression of resistance to schistosomiasis mansoni through interactions autologous immune functions derived from an induced anti-idiotypic response. This anti-clonotypic response is by immunization with syngeneic L3T4+ receptor-bearing lymphoblasts and for sake description termed "auto-anti-idiotypic". It antigenically restricted cannot be allogeneic cells. Anti-idiotypic profoundly suppressed development protective immunity after exposure irradiated cercariae...
Abstract Introduction: Metastatic Ewing sarcoma (ES) has a dismal prognosis, and clarifying the molecular cellular mechanism of metastasis is most pressing need to improving prognosis. Recently, heterogenous expression driving fusion gene EWS-FLI1, as well canonical Wnt/β-catenin signaling activity been increasingly recognized potential mediator metastatic progression. However, targeting pathway difficult due its complexity cross-activity, not much known how ES cells maintain Wnt...