A5003511586- Mass Spectrometry Techniques and Applications
- Ion-surface interactions and analysis
- Analytical chemistry methods development
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Toxic Organic Pollutants Impact
- Atmospheric chemistry and aerosols
- Per- and polyfluoroalkyl substances research
- Nuclear physics research studies
- Atomic and Molecular Physics
- Nuclear Physics and Applications
- Chemical Synthesis and Analysis
- Advanced Chemical Physics Studies
- Microbial Community Ecology and Physiology
- Carbohydrate Chemistry and Synthesis
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Advanced Materials Characterization Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Redox biology and oxidative stress
- DNA and Nucleic Acid Chemistry
- Astronomical and nuclear sciences
- Electrohydrodynamics and Fluid Dynamics
- Protist diversity and phylogeny
- X-ray Spectroscopy and Fluorescence Analysis
Michigan State University
2023
Central Michigan University
2014-2022
Oregon State University
2005-2017
German Cancer Research Center
2010
Heidelberg University
2010
Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences
2007-2008
Swiss Federal Institute of Aquatic Science and Technology
2008
Division of Chemistry
2007
Stanford University
1989-2006
Ionwerks (United States)
2003-2004
Fluorochemicals have widespread applications and are released into municipal wastewater treatment plants via domestic wastewater. A field study was conducted at a full-scale plant to determine the mass flows of selected fluorochemicals. Flow-proportional, 24 h samples raw influent, primary effluent, trickling filter secondary final effluent grab primary, thickened, activated, anaerobically digested sludge were collected over 10 days analyzed by liquid chromatography electrospray-ionization...
Aqueous film-forming foams (AFFF) are complex mixtures containing fluorocarbon- and hydrocarbon-based surfactants that used to fight hydrocarbon-fueled fires. The military is the largest consumer of AFFF in United States, fire-training activities conducted at bases have led groundwater contamination by unspent fuels chemicals. A direct-injection, liquid-chromatography tandem mass spectrometry (LC MS/MS) method was developed quantify a suite fluorotelomer sulfonate collected from where were...
Fluorinated alkyl substances, which include perfluorooctane sulfonate (PFOS), constitute a diverse class of chemicals that occur in wide range products. Concern about the presence fluorinated substances environment has increased since PFOS and related fluoroalkyl were detected blood plasma nonoccupationally exposed humans animal tissues collected from around globe, including sparsely populated regions have no apparent sources. was also found to exhibit both bioaccumulative toxic properties....
Oligomers possessing the Morpholino phosphorodiamidate backbone were evaluated for resistance to a variety of enzymes and biologic fluids. A 25-mer was incubated with nucleases, proteases, esterases, serum, reaction mixtures directly analyzed by MALDI-TOF mass spectrometry. The completely resistant 13 different hydrolases serum plasma. excellent phosphorodiamidates enzymatic attack indicates their suitability in vivo use.
A quantitative method was developed for the determination of fluorinated alkyl substances in municipal wastewater influents and effluents. The consisted centrifugation followed by large-volume injection (500 μL) supernatant onto a liquid chromatograph with reverse-phase column detection electrospray ionization tandem mass spectrometry. analytes studied include perfluoroalkyl sulfonates, fluorotelomer perfluorocarboxylates, select sulfonamides. Recoveries from treatment plant (WWTP) raw final...
Fluorochemicals are persistent contaminants that globally distributed in air, water, sediments, and biota. Wastewater treatment plants (WWTPs) play an important role mitigating pollutant releases from municipalities to aquatic terrestrial environments. However, because WWTPs point sources of fluorochemicals, it is understand their contribution fluorochemical burdens the greater context watersheds. To this end, over a 1 week period, mass flows 11 fluorochemicals seven discharge effluent into...
Semivolatile fluorinated organic compounds (FOCs) were measured in archived air sample extracts collected from Hedo Station Observatory (HSO) on Okinawa, Japan and Mount Bachelor (MBO), Oregon U.S. during the springs of 2004 (MBO HSO) 2006 (MBO). Fluorotelomer alcohols (FTOHs) both Asian western masses, though masses had significantly higher concentrations. Concentrations fluorotelomer olefins 8:2 acrylate reported for first time. N-ethyl perfluorooctane sulfonamide, N-methyl sulfonamido...
"Candidatus Pelagibacter ubique," an abundant marine alphaproteobacterium, subsists in nature at low ambient nutrient concentrations and may often be exposed to limitation, but its genome reveals no evidence of global regulatory mechanisms for adaptation stationary phase. High-resolution capillary liquid chromatography coupled online LTQ mass spectrometer was used build accurate time (AMT) tag library that enabled quantitative examination proteomic differences between exponential-...
The gas-phase structures and fragmentation pathways of the singly protonated peptide arginylglycylaspartic acid (RGD) are investigated by means collision-induced-dissociation (CID) detailed molecular mechanics density functional theory (DFT) calculations. It is demonstrated that despite ionizing proton being strongly sequestered at guanidine group, RGD can easily be fragmented on charge directed pathways. This due to facile mobilization C-terminal or aspartic COOH protons thereby generating...
Fluorescein was covalently attached through a cystamine linker group to carboxy-derivatized polyacrylamide microspheres generate phagocytosable particles containing fluorescent reporter groups. A unique feature of these beads is that the dye recoverable in near-quantitative yield from intracellular environments by thiol reduction disulfide bond. Fluorescence microscopy indicated individual neutrophils could bind as many ∼20 serum-opsonized beads, although no appreciable cellular association...
Abstract We used a proteomic approach to identify novel proteins that may regulate metabotropic glutamate receptor 5 (mGluR5) responses by direct or indirect protein interactions. This does not rely on the heterologous expression of and offers advantage identifying interactions in native environment. The mGluR5 was immunoprecipitated from rat brain lysates; co‐immunoprecipitating were analyzed mass spectrometry identified peptides matched databases determine correlating parent proteins....
Summary Besides 19 potential reductive dehalogenase genes, the genome of Dehalococcoides ethenogenes strain 195 contains over 60 genes annotated as encoding oxidoreductases, including five hydrogenase complexes and a formate dehydrogenase (Fdh). Using quantitative reverse transcriptase polymerase chain reaction, we found that periplasmic Hup Fdh were most highly expressed in batch‐grown pure cultures, which H 2 partial pressure was > 0.1 atm, butyrate/tetrachloroethene‐mixed pressures 10...
Proteins from Escherichia coli were isolated based on their ability to bind DNA and digested in-solution with trypsin; the resulting peptides separated using HPLC subsequently analyzed MALDI TOF/TOF ESI Q-TOF instruments. Various properties of observed two ionization techniques compared taking into account differences between mass analyzers. This empirical analysis a data set containing hundreds thousands individual amino acids supports some currently held notions regarding complementary...
Summary Ecological studies indicate that aerobic anoxygenic phototrophic bacteria (AAP) use bacteriochlorophyll to support electron transport are widely distributed in the oceans. All cultivated marine AAP alpha‐3 and alpha‐4 Proteobacteria , but metagenomic evidence indicates uncultured Gammaproteobacteria important members of ocean surface microbial communities. Here we report description obligately oligotrophic, have genes for photosynthesis. Three strains belonging OM60 clade were...
Resonant electron capture mass spectra of aliphatic and aromatic amino acids their methyl esters show intense [M−H]- negative ions in the low-energy range. Ion formation results from a predissociation mechanism mediated by resonant state. Methylation general has little influence on electronic structure according to quantum chemical calculations, but corresponding esters, [M−Me]-, could be ascertained arise only at higher resonance energies. Aromatic are characterized an additional...
ABSTRACT The filamentous virion of the closterovirus Beet yellows virus (BYV) consists a long body formed by major capsid protein (CP) and short tail composed minor (CPm) virus-encoded Hsp70 homolog. By using nano-liquid chromatography-tandem mass spectrometry biochemical analyses, we show here that BYV 64-kDa (p64) is fourth integral component virions. N-terminal domain p64 exposed at surface accessible to antibodies mild trypsin digestion. In contrast, C-terminal embedded in inaccessible...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSequencing procyanidin oligomers by fast atom bombardment mass spectrometryJoseph J. Karchesy, Richard W. Hemingway, Yeap L. Foo, Elisabeth. Barofsky, and Douglas F. BarofskyCite this: Anal. Chem. 1986, 58, 12, 2563–2567Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/ac00125a044https://doi.org/10.1021/ac00125a044research-articleACS...
Abstract With the phaseout of manufacture some polybrominated diphenyl ether (PBDE) formulations, namely penta‐brominated (BDE) and octa‐BDE, continued use deca‐BDE formulation, it is important to be able predict photodegradation more highly brominated congeners. A model was developed validated products their relative concentrations from PBDEs. The enthalpies formation 209 PBDE congeners were calculated, reaction rate constants obtained. predicted for PBDEs show linear correlation with...
Abstract The Facility for Rare Isotope Beams (FRIB) began operation with 1 kW beam power scientific users in May 2022 upon completion of 8 years project construction. ramp-up to the ultimate 400 kW, planned over a 6-year period, will enable facility reach its full potential discovery isotope science and applications. In December 2023, record-high 10.4 uranium was delivered target. Technological developments accelerator improvements are being made entire key ramp-up. Major technological...
Abstract Massive‐cluster impact is demonstrated to be an effective ionization technique for the mass analysis of proteins as large 17 kDa. The design cluster source permits coupling both magnetic‐sector and quadrupole spectrometers. Mass spectra are characterized by almost total absence chemical background a predominance multiply charged ions formed from 100% glycerol matrix. number charge states produced observed range +3 +9 chicken egg lysozyme (14 310 Da). lower m/z values provided higher...