A5024104987- Acute Myeloid Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hematological disorders and diagnostics
- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Calcium signaling and nucleotide metabolism
- Long-Term Effects of COVID-19
- Viral-associated cancers and disorders
- Lysosomal Storage Disorders Research
- Trypanosoma species research and implications
- Studies on Chitinases and Chitosanases
- Hematopoietic Stem Cell Transplantation
- Breastfeeding Practices and Influences
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Child Nutrition and Feeding Issues
- Synthesis and Biological Evaluation
- Acute Lymphoblastic Leukemia research
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Cancer therapeutics and mechanisms
Université Grenoble Alpes
2019-2024
Centre Hospitalier Universitaire de Grenoble
2018-2024
Centre National de la Recherche Scientifique
2021-2024
Institut pour l'avancée des biosciences
2021-2024
Inserm
2021-2024
Institut de Biosciences et Biotechnologies
2021
Centre Hospitalier Universitaire de Clermont-Ferrand
2015-2017
UPMC Hillman Cancer Center
2005-2008
University of Pittsburgh
2005
Tomsk National Research Medical Center
1999
Abstract The effect of adenosine and its analogues on the cytotoxic activity IL-2-activated NK cells was investigated. Adenosine is an endogenous ligand for four different receptor (AdoR) subtypes (AdoRA1, AdoRA2A, AdoRA2B, AdoRA3). Increased concentrations were found in ascites MethA sarcoma or culture medium 3LL Lewis lung carcinoma growing under hypoxic conditions. We hypothesize that intratumor impairs ability lymphokine-activated killer (LAK) to kill tumor cells. AdoR engagement LAK...
Adenosine is an important signaling molecule that regulates multiple physiologic processes and exerts major anti-inflammatory actions. Tumors have high concentrations of adenosine, which could inhibit the function tumor-infiltrating lymphoid cells. We investigated ability adenosine its stable analogue 2-chloroadenosine (CADO) to cytokine production cytotoxic activity lymphokine-activated killer (LAK) cells determined whether both these effects are initiated via a common pathway. CADO...
Abstract The goal of this study was to investigate the effects adenosine and its stable analogue 2-chloroadenosine (CADO) on cytotoxic activity cytokine production by human antimelanoma specific CD8+ CD4+ T-helper type 1 (Th1) clones. T cells inhibited CADO. Using Lab MAP multiplex technology, we found that inhibits various cytokines chemokines cells. Studies with CGS21680, a agonist A2A receptor (AdoRA2A), ZM241385, an AdoRA2-selective antagonist, indicate inhibitory are mediated via cyclic...
A precise identification and phenotypic characterization of human B-cell subsets is crucial importance in both basic research medicine. In the literature, flow cytometry studies for B-lymphocytes are mainly focused on description a particular cell stage, or specific stages observed single type sample. present work, we propose backbone 6 antibodies (CD38, CD27, CD10, CD19, CD5 CD45) an efficient gating strategy to identify, analysis tube, large number covering whole differentiation from...
The SARS-CoV-2 infection triggers excessive immune response resulting in increased levels of pro-inflammatory cytokines, endothelial injury, and intravascular coagulopathy. complement system (CS) activation participates to this hyperinflammatory response. However, it is still unclear which pathways (classical, alternative, or lectin pathway) pilots the effector mechanisms that contribute critical illness. To better understand correlates disease severity, we performed an analysis CS...
Venetoclax-azacitidine is the standard of treatment for unfit acute myeloid leukemia patients. In VIALE-A study, was given until progression but there are no data on its optimal duration responding patients who do not tolerate indefinite therapy. We retrospectively analyzed outcome discontinued venetoclax or venetoclax-azacitidine due to poor tolerance. Sixty-two newly diagnosed (ND) AML and 22 with morphological relapse refractory were included. ND cohort (n = 62), 28 stopped azacitidine 34...
Background Studies of normal bone marrow (BM) cell composition by flow cytometry are scarce. Presently, we aimed to quantify 14 subsets from infants elderly patients. Methods Cell in BM samples 180 individuals without morphologically abnormal leukocytes were analyzed using a single combination eight antibodies: CD3/CD10/CD38 / CD19/CD36/CD16/CD34/CD45. Results By comparison with the Holdrinet score, first validated immature granulocyte/neutrophil (IGRA/N) ratio as readily obtainable...
Suspicion of myelodysplastic syndromes (MDS) is one the commonest reasons for bone marrow aspirate in elderly patients presenting with persistent peripheral blood (PB) cytopenia unclear etiology. A PB assay that accurately rules out MDS would have major benefits. The diagnostic accuracy intra-individual robust coefficient variation (RCV) neutrophil myeloperoxidase (MPO) expression measured by flow cytometric analysis was evaluated a retrospective derivation study (44 cases and 44 controls)...
Diffuse large B-cell lymphoma (DLBCL) is a clinically heterogeneous entity, in which the first-line treatment currently consists of an immuno-chemotherapy regimen (R-CHOP). However, around 30% patients will not respond or relapse. Overexpression c-MYC p53 frequently found DLBCL, but association with prognosis remains controversial, as for other biomarkers previously linked DLBCL aggressivity (CD5, CD23, BCL2). The aim this study was to explore expression these and their correlation outcome,...
R-CHOP immuno-chemotherapy significantly improved clinical management of diffuse large B-cell lymphoma (DLBCL). However, 30-40% DLBCL patients still present a refractory disease or relapse. Most the prognostic markers identified to date fail accurately stratify high-risk patients. We have previously shown that nuclear protein CYCLON is associated with progression and resistance anti-CD20 immunotherapy in preclinical models. also recently reported it represents potent predictor relapse...
COVID-19 is caused by the human pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has resulted in widespread morbidity mortality. CD4 + T cells, CD8 cells neutralizing antibodies all contribute to control SARS-CoV-2 infection. However, heterogeneity a major factor disease severity immune innate adaptive responses SARS-CoV-2. We performed deep analysis flow cytometry of lymphocyte populations 125 hospitalized infected patients on day hospital admission. Five clusters...
High-grade B-cell lymphoma with rearrangements of MYC and BCL2 and/or BCL6 is an aggressive mature neoplasm, whereas B-lymphoblastic immature cell proliferation, a frequent positivity for terminal deoxynucleotidyl transferase. The transformation low-grade follicular into lymphoblastic neoplasm expressing transferase very rare event.A 55-year-old Caucasian man was followed grade 1-2 carrying t(14;18) IGH/BCL2+ initially treated R-CHOP. presented two relapses. In the third relapse, patient had...