A5088670019- Complement system in diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Renal Diseases and Glomerulopathies
- Cell Adhesion Molecules Research
- Urticaria and Related Conditions
- Autoimmune Bullous Skin Diseases
- Immune Cell Function and Interaction
- Prion Diseases and Protein Misfolding
- SARS-CoV-2 and COVID-19 Research
- Vitamin K Research Studies
- Chronic Lymphocytic Leukemia Research
- Platelet Disorders and Treatments
- Hematological disorders and diagnostics
- Acute Myeloid Leukemia Research
- Fungal Infections and Studies
- Atherosclerosis and Cardiovascular Diseases
- Reproductive System and Pregnancy
- COVID-19 Clinical Research Studies
- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
Centre Hospitalier Universitaire de Grenoble
2015-2025
Centre National de la Recherche Scientifique
2011-2025
Université Grenoble Alpes
2015-2025
Institut de Biologie Structurale
2021-2025
CEA Grenoble
2021-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2025
Institut pour l'avancée des biosciences
2016-2025
Inserm
2016-2025
Translational Innovation in Medicine and Complexity
2016-2022
Laboratoire National de Référence
2018
Most patients with idiopathic membranous nephropathy (IMN) have IgG4 autoantibodies against phospholipase A2 receptor (PLA2R). C3 and C5b-9 are found in immune deposits of IMN kidney biopsy specimens, but the pathway complement activation remains elusive. We report case a patient who developed intense staining for PLA2R, IgG4, C3, C5b-9, factor B, properdin very weak C1q, C4d, IgG1. Measurement mannan binding lectin (MBL) antigenic level activity revealed MBL deficiency. Genotyping...
Blast quantification in the bone marrow (BM) is crucial for evaluating myeloid neoplasms, with cytomorphology being only recognized analysis. The CD34 cell (CD34M) count by flow cytometry promising but impaired BM hemodilution. A modified version of Holdrinet index (mHI) routinely used to assess it, though not yet validated. By analyzing two differently hemodiluted samples from 51 patients suspicion myelodysplasia, this study confirms mHI accuracy assessing white blood purity. mHI-adjusted...
Ficolins are oligomeric innate immune recognition proteins consisting of a collagen-like region and fibrinogen-like domain that bind to pathogen- apoptotic cell-associated molecular patterns. To investigate their carbohydrate binding specificities, serum-derived L-ficolin recombinant H- M-ficolins were fluorescently labeled, ability was analyzed by glycan array screening. preferentially recognized disulfated N-acetyllactosamine tri- tetrasaccharides containing terminal galactose or...
Abstract Ficolins and pentraxins are soluble oligomeric pattern-recognition molecules that sense danger signals from pathogens altered self-cells might act synergistically in innate immune defense maintenance of tolerance. The interaction M-ficolin with the long pentraxin 3 (PTX3) has been characterized using surface plasmon resonance spectroscopy electron microscopy. was shown to bind PTX3 high affinity presence calcium ions. abolished EDTA inhibited by N-acetyl-D-glucosamine, indicating...
L- and H-ficolins are serum oligomeric defense proteins consisting of a collagen-like region fibrinogen-like recognition domain that bind to pathogen- apoptotic cell-associated molecular patterns. They share with mannan-binding lectin (MBL) the ability associate MBL-associated serine proteases (MASP)-1, -2, -3, protein MAp19 trigger complement pathway through MASP-2 activation. Recent studies have revealed essential role Lys(55) in collagenous MBL interaction MASPs calreticulin (CRT). To...
A precise identification and phenotypic characterization of human B-cell subsets is crucial importance in both basic research medicine. In the literature, flow cytometry studies for B-lymphocytes are mainly focused on description a particular cell stage, or specific stages observed single type sample. present work, we propose backbone 6 antibodies (CD38, CD27, CD10, CD19, CD5 CD45) an efficient gating strategy to identify, analysis tube, large number covering whole differentiation from...
The SARS-CoV-2 infection triggers excessive immune response resulting in increased levels of pro-inflammatory cytokines, endothelial injury, and intravascular coagulopathy. complement system (CS) activation participates to this hyperinflammatory response. However, it is still unclear which pathways (classical, alternative, or lectin pathway) pilots the effector mechanisms that contribute critical illness. To better understand correlates disease severity, we performed an analysis CS...
Abstract Background When a member of family has been diagnosed with hereditary angioedema (HAE) before child is born, the question early diagnosis arises. Indeed, first attacks may occur at birth. Early complicated by biological issues. Due to immaturity complement system, C1 Inhibitor (C1 INH) and C4 levels can be low birth, generally in range 60 100% adult reference values. Like most proteins, their normalize after one year life. However, this not always case, we report two...
Abstract Innate immunity is the major host defense against invasive aspergillosis. To determine whether collectin mannan-binding lectin (MBL) involved in initial protective through complement activation opportunistic fungal infections caused by Aspergillus, we performed vitro studies on 29 different strains of Aspergillus conidia from five species. Incubation human normal serum leads to alternative pathway, whereas neither classical nor pathways C4 and C2 cleavage are activated. Complement...
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of multiple autoantibodies. Antibodies against Ficolin-3 were previously identified in sera some SLE patients, but their prevalence and significance have not been yet investigated. The aims this study to determine anti-ficolin-3 antibodies among patients investigate potential as diagnostic and/or prognostic biomarkers SLE. In retrospective study, from (n = 165) selected preexisting declared...
Summary The overall role of complement in the host–pathogen relationship is now well understood. However, its involvement at a chronic stage infection, such as hepatitis C, less documented. Here, results are reported which point to use specific C4 monitoring follow-up HCV patients. This study concerns 66 patients with treated interferon alpha 2b alone or + ribavirin, and 50 healthy adults controls. Complement blood tests were performed measure C1q, C3, C4, mannan binding lectin (MBL), C1s-C1...
A growing number of studies have investigated the interaction between C1q and PrP, but oligomeric form PrP involved in this remains to be determined. Aggregation recombinant full-length murine presence 100 mm NaCl allowed us isolate three different types oligomers by size-exclusion chromatography. In contrast monomers fibrils, these activate classical complement pathway, smallest species containing 8–15 protomers being most efficient. We used Thioflavine T fluorescence monitor aggregation...
Systemic lupus erythematosus (SLE) is a multisystem inflammatory disease characterized by the production of various autoantibodies. The aim this study was to investigate presence anti-ficolin-2 antibodies in SLE patients and evaluate association between levels these autoantibodies, clinical manifestations, activity.This comparative using cohort 165 48 healthy subjects. were further divided into 2 groups (low activity [SLE Disease Activity Index (SLEDAI) score ≤4, n = 88] high [SLEDAI >4,...
Background: Acquired angioedemas are divided into type I associated with lymphoproliferation and II caused by anti-C1-inhibitor antibodies. Recent reports have suggested that this distinction is not so clear-cut, mainly because of the presence antibodies against C1 inhibitor in some cases belonging to group. We report herein 2 additional acquired angioedema antibody. Material Methods: One man 1 woman had for several years. In man, a monoclonal component been detected years before present...
Growth arrest and DNA damage-inducible gene 34 (GADD34) is an inducible cofactor of protein phosphatase 1, which has important role in the unfolded response. GADD34 been shown to be necessary for type I interferon proinflammatory cytokine production response viral infection murine models. We investigate expression rheumatoid arthritis (RA), cytokines have pathogenic role. The objective this study was evaluate potential as a biomarker RA patients. report case-control on peripheral blood...