A5048210189- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Plant nutrient uptake and metabolism
- Chalcogenide Semiconductor Thin Films
- Semiconductor materials and interfaces
- Algal biology and biofuel production
- Thin-Film Transistor Technologies
- Advanced biosensing and bioanalysis techniques
- Conducting polymers and applications
- Ferroelectric and Piezoelectric Materials
- Pesticide and Herbicide Environmental Studies
- Neuroscience and Neural Engineering
- Cancer Research and Treatment
- Internet of Things and Social Network Interactions
- Lipid metabolism and biosynthesis
- RNA and protein synthesis mechanisms
- Anodic Oxide Films and Nanostructures
- Electrocatalysts for Energy Conversion
- Artificial Immune Systems Applications
- Endoplasmic Reticulum Stress and Disease
Korea University
2006-2024
University of Groningen
2014-2020
Namseoul University
2004-2012
Florida State University
1989
Louisiana State University
1987
Engineering of Saccharomyces cerevisiae for the simultaneous utilization hexose and pentose sugars is vital cost-efficient cellulosic bioethanol production. This yeast lacks specific transporters depends on endogenous low affinity uptake. Consequently, engineered xylose-fermenting strains first utilize D-glucose before D-xylose can be transported metabolized. We have used an evolutionary engineering approach that a quadruple hexokinase deletion S. strain to select growth in presence high...
The yeast Saccharomyces cerevisiae is unable to ferment pentose sugars like d-xylose. Through the introduction of respective metabolic pathway, S. able xylose but first utilizes d-glucose before d-xylose can be transported and metabolized. Low affinity uptake occurs through endogenous hexose (Hxt) transporters. For a more robust sugar fermentation, co-consumption desired as fermentation in particular prone inhibition by compounds present pretreated lignocellulosic feedstocks.Evolutionary...
Engineering of the yeast Saccharomyces cerevisiae for improved utilization pentose sugars is vital cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engineered into specific transporters, they remain subjected to glucose-regulated protein degradation. Therefore, in absence glucose or when exhausted from medium, some Hxt proteins with high xylose transport capacity are rapidly degraded and removed cytoplasmic membrane. Thus, turnover such...
ABSTRACT Engineering Saccharomyces cerevisiae for the utilization of pentose sugars is an important goal production second-generation bioethanol and biochemicals. However, S. lacks specific transporters, in presence glucose, pentoses enter cell inefficiently via endogenous hexose transporters (HXTs). By means vivo engineering, we have developed a quadruple hexokinase deletion mutant that evolved into strain efficiently utilizes d -xylose high -glucose concentrations. A genome sequence...
ABSTRACT Hxt2 is a glucose repressed, high affinity transporter of the yeast Saccharomyces cerevisiae and subjected to induced degradation. Hxt11 sugar that stably expressed at membrane irrespective concentration. To transfer this property Hxt2, N‐terminal tail was replaced by corresponding region yielding chimeric Hxt11/2 transporter. This resulted in stable expression improved growth on 8% d ‐glucose 4% ‐xylose. Mutation N361 into threonine reversed specificity for ‐xylose over with...
A sandwich-type immunosensor composed of antigen−double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement fluorescence intensity and quantitative analysis single-stranded DNA based on concentration free glyphosate. The reaction between DNA−gold NPs immobilized antibody substrate carried out 2 h. results antigen−antibody were measured basis obtained from comparison with antigens at concentrations 0.01−100 μg mL−1 detection NPs. For glyphosate(0.01−100 mL−1),...
Optimizing D‐xylose transport in Saccharomyces cerevisiae is essential for efficient bioethanol production from cellulosic materials. We have used a gene shuffling approach of hexose (Hxt) transporters order to increase the affinity D‐xylose. Various libraries were transformed transporter deletion strain, and shuffled genes selected via growth on low concentrations This screening yielded two homologous fusion proteins (fusions 9,4 9,6), both consisting major central part Hxt2 various smaller...
ABSTRACT Optimizing D-xylose consumption in Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. An evolutionary engineering approach was used to elevate a xylose-fermenting S. strain carrying the D-xylose-specific N367I mutation endogenous chimeric Hxt36 hexose transporter. This carries quadruple hexokinase deletion that prevents glucose utilization, and allows selection of improved growth rates on presence high D-glucose concentrations. Evolutionary...
Abstract We report the fabrication of organic thin‐film transistors (OTFTs) with high‐ k gate dielectrics Mn‐doped Bi 2 Ti O 7 (BTO) films. 3% BTO films deposited on polymer substrates by pulsed laser deposition at room temperature exhibit low leakage currents 2.1 × 10 –8 A/cm an applied electric field 0.3 MV/cm, while undoped show much higher 4.3 –4 . Mn doping effectively reduces number oxygen vacancies in and improves electrical properties. Low operation voltage significantly reduced are...