A5086614084- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Diet and metabolism studies
- Receptor Mechanisms and Signaling
- Growth Hormone and Insulin-like Growth Factors
- melanin and skin pigmentation
- Neuroendocrine regulation and behavior
- Tryptophan and brain disorders
- Thyroid Disorders and Treatments
- Hypothalamic control of reproductive hormones
- Neuropeptides and Animal Physiology
- Dietary Effects on Health
Instituto Multidisciplinario de Biología Celular
2015-2024
Comisión de Investigaciones Científicas
2017-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2024
Universidad Nacional de La Plata
2017-2023
Institute of Cell Biology
2023
Biochemistry Research Institute of La Plata
2017
The ghrelin receptor or growth hormone secretagogue (GHSR) is a G-protein-coupled that controls and insulin secretion, food intake, reward-seeking behaviors. Liver-expressed antimicrobial peptide 2 (LEAP2) was recently described as an endogenous antagonist of GHSR. Here, we present study aimed at delineating the structural determinants required for LEAP2 activity toward We demonstrate entire sequence not necessary its actions. Indeed, N-terminal part alone confers binding to LEAP2. found...
Ghrelin is a stomach-derived hormone that regulates food intake and neuroendocrine function by acting on its receptor, GHSR (Growth Hormone Secretagogue Receptor). Recent evidence indicates key of ghrelin to signal stress the brain. It has been suggested one potential stress-related targets CRF (Corticotropin-Releasing Factor)-producing neurons hypothalamic paraventricular nucleus, which secrete neuropeptide into median eminence activate hypothalamic-pituitary-adrenal axis. However, neural...
Ghrelin is an octanoylated peptide hormone that potently and rapidly increases food intake. The orexigenic action of ghrelin involves the hypothalamic arcuate nucleus (ARC), which accessible to plasma expresses high levels receptor. Local administration in a variety other brain nuclei also It currently unclear, however, whether these non-ARC targets are impacted by physiological ghrelin. Thus, present study aimed clarify participate short-term actions First, c-Fos induction into mouse brains...
Ghrelin is a stomach-derived octanoylated peptide hormone that plays variety of well-established biological roles acting via its specific receptor known as growth secretagogue (GHSR). In plasma, des-octanoylated form ghrelin, named des-acyl ghrelin (DAG), also exists. DAG suggested to be signalling molecule has targets, including the brain, and regulates some physiological functions. However, no for been reported until now, and, consequently, potential role remained matter debate. present...
The growth hormone secretagogue receptor type 1a (GHSR1a) has the highest known constitutive activity of any G protein–coupled (GPCR). GHSR1a mediates action ghrelin, and its activation increases transcriptional electrical in hypothalamic neurons. Although is present at GABAergic presynaptic terminals, effect on neurotransmitter release remains unclear. activities voltage-gated calcium channels, CaV2.1 CaV2.2, which mediate are modulated by many GPCRs. Here, we show that both...
Abstract Ghrelin is a potent orexigenic peptide hormone that acts through the growth secretagogue receptor (GHSR), G protein–coupled highly expressed in hypothalamus. In vitro studies have shown GHSR displays high constitutive activity, whose physiological relevance uncertain. As gene expression hypothalamus known to increase fasting conditions, we tested hypothesis activity at hypothalamic level drives fasting-induced hyperphagia. We found refed wild-type (WT) mice displayed robust...
Ghrelin is known to act on the area postrema (AP), a sensory circumventricular organ located in medulla oblongata that regulates variety of important physiological functions. However, neuronal targets ghrelin AP and their potential role are currently unknown. In this study, we used wild-type genetically modified mice gain insights into neurons expressing receptor [growth hormone secretagogue (GHSR)] role. We show circulating mainly accesses but not adjacent nucleus solitary tract. Also, both...
Ghrelin exists in two forms circulation, acyl ghrelin and des-acyl ghrelin, both of which have distinct fundamental roles a variety physiological functions. Despite this fact, large proportion papers simply measure refer to plasma without specifying the acylation status. It is therefore critical assess state status all studies. In study we tested effect administration on hypothalamic-pituitary-adrenal axis anxiety-like behavior mice lacking endogenous ghrelin-O-acyltransferase (GOAT)...
Fasting down-regulates the hypothalamus-pituitary-thyroid (HPT) axis activity through a reduction of TRH synthesis in neurons parvocellular paraventricular nucleus hypothalamus (PVN). These project to median eminence (ME), where terminals are close cytoplasmic extensions β2 tanycytes. Tanycytes express pyroglutamyl peptidase II (PPII), TRH-degrading ectoenzyme that controls amount reaches anterior pituitary. We tested hypothesis regulation ME PPII is another mechanism by which fasting...
Arcuate nucleus (ARC) neurons producing Agouti-related peptide (AgRP) and neuropeptide Y (NPY; ARCAgRP/NPY neurons) are activated under energy-deficit states. innervate the hypothalamic paraventricular (PVH), ARC→PVH projections recognized as key regulators of food intake. Plasma ghrelin levels increase states activate by acting on growth hormone secretagogue receptor (GHSR). Here, we hypothesized that activation in fasted mice would promote morphological remodeling ARCAgRP/NPY→PVH a...
Abstract The hormone ghrelin displays several well-characterized functions, including some with pharmaceutical interest. receptor for ghrelin, the growth secretagogue (GHSR), is expressed in hypothalamic paraventricular nucleus (PVH), a critical hub integration of metabolic, neuroendocrine, autonomic, and behavioral functions. Here, we performed neuroanatomical functional characterization neuronal types mediating actions PVH male mice. We found that fluorescent mainly labels neurons...
The melanocortin 4 receptor (MC4R) is a G protein-coupled involved in food intake and energy expenditure regulation. MC4R activation modifies neuronal activity but the molecular mechanisms by which this regulation occurs remain unclear. Here, we tested hypothesis that regulates of voltage-gated calcium channels and, as consequence, synaptic activity. We also whether proposed effect amygdala, brain area known to mediate anorexigenic actions signaling. Using patch-clamp technique, found with...
Abstract The growth hormone secretagogue receptor (GHSR), primarily known as the for hunger ghrelin, potently controls food intake, yet specific Ghsr-expressing cells mediating orexigenic effects of this remain incompletely characterized. Since Ghsr is expressed in gamma-aminobutyric acid (GABA)–producing neurons, we sought to investigate whether selective expression a subset GABA neurons sufficient mediate GHSR's on feeding. First, crossed mice that express tamoxifen-dependent Cre...