A5089240010- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Chromosomal and Genetic Variations
- Renal and related cancers
- Plant nutrient uptake and metabolism
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Nanoplatforms for cancer theranostics
- Sexual Differentiation and Disorders
- Lung Cancer Research Studies
- RNA Research and Splicing
- Sphingolipid Metabolism and Signaling
- Nanoparticle-Based Drug Delivery
- Nuclear Structure and Function
- Estrogen and related hormone effects
- Ovarian function and disorders
- Cellular transport and secretion
- Neuropeptides and Animal Physiology
China Medical University
2020
Institute of Biomedical Sciences, Academia Sinica
2009-2020
Academia Sinica
2013
Baylor College of Medicine
2003-2011
Institut de Biologie Moléculaire et Cellulaire
2006-2007
University of Nevada, Reno
2007
Cellular Research (United States)
2006
Cytokinesis in somatic cells concludes with the formation of a midbody, which is abscised to form individual daughter cells. In contrast, germ cell cytokinesis results permanent intercellular bridge connecting through large cytoplasmic channel. During spermatogenesis, proposed roles for include communication, synchronization, and chromosome dosage compensation haploid Although several essential components midbody have recently been identified, vertebrate until now not described. Herein, we...
Zona pellucida binding protein 1 (ZPBP1), a spermatid and spermatozoon that localizes to the acrosome, was originally identified in pigs named for its oocyte zona pellucida.In an silico search germ cell-specific genes, Zpbp1 novel paralog, Zpbp2, were discovered confirmed be expressed only testes both mice humans.To study vivo functions of ZPBP proteins, we disrupted Zpbp2 mice.Males lacking ZPBP1 sterile, with abnormal round-headed sperm morphology no forward motility.Ultrastructural...
We previously isolated Aurora-C/Aie1 in a screen for kinases expressed mouse sperm and eggs. Here, we show the localization of endogenous Aurora-C examine its roles during female meiosis. was detected at centromeres along chromosome arms prometaphase I-metaphase I concentrated metaphase II, which also phosphorylated Thr171. During anaphase I-telophase transition, dephosphorylated relocalized to midzone midbody. Microinjection kinase-deficient (AurC-KD) mRNA into oocytes significantly...
Centriole duplication begins with the formation of a single procentriole next to preexisting centriole. CPAP (centrosomal protein 4.1–associated protein) was previously reported participate in centriole elongation. Here, we show that CEP120 is cell cycle–regulated directly interacts and required for duplication. levels increased gradually from early S G2/M decreased significantly after mitosis. Forced overexpression either or not only induced assembly overly long centrioles but also produced...
Sperm flagellar motion is the outcome of a dynamic interplay between axonemal cytoskeleton, peri-axonemal accessory structures, and multiple regulatory networks that coordinate to produce beat waveform. Tektins are conserved components proteome in evolutionarily diverse species believed play essential roles mechanics sperm motility. Using database mining, we identified new paralogs previously annotated tektins, including tektin 4 (TEKT4), which shares 77.1% identity with its nearest human...
Abstract Tektins are evolutionarily conserved flagellar (and ciliary) filamentous proteins present in the axoneme and peri‐axonemal structures diverse metazoan species. We have previously shown that tektin 3 (TEKT3) 4 (TEKT4) male germ cell‐enriched proteins, TEKT4 is essential for coordinated progressive sperm motility mice. Here we report mice null TEKT3 produce with reduced (47.2% motility) forward progression, increased structural bending defects. Male TEKT3‐null however maintain normal...
Docetaxel-based chemotherapy for prostate cancer is the clinical standard of care. However, nonspecific targeting, multiple drug resistance, and adverse side effects are common obstacles. Various natural compounds, including epigallocatechin-3-gallate (EGCG) in combination with taxane, have potential to be developed as anticancer therapeutics. Although synergistic hydrophobic-hydrophilic drugs been used some success, main drawbacks this approach poor bioavailability, unfavorable...
Abstract Mutations in many centriolar protein-encoding genes cause primary microcephaly. Using super-resolution and electron microscopy, we find that the human microcephaly protein, RTTN, is recruited to proximal end of procentriole at early S phase, located inner luminal walls centrioles. Further studies demonstrate RTTN directly interacts with STIL acts downstream STIL-mediated centriole assembly. CRISPR/Cas9-mediated gene knockout p53-deficient cells induce amplification primitive bodies...
Primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by small brain size with mental retardation. CPAP (also known as CENPJ), microcephaly-associated gene, plays key role in centriole biogenesis. Here, we generated previously unreported conditional knockout allele the mouse Cpap gene. Our results showed that deletion central nervous system preferentially induces formation of monopolar spindles radial glia progenitors (RGPs) at around embryonic day 14.5 and causes robust...
Through in silico subtraction and microarray analysis, we identified mouse Gpr149, a novel, oocyte-enriched transcript that encodes predicted orphan G-protein-coupled receptor (GPR). Phylogenetic analysis of GPR149 from fish to mammals suggests it is widely conserved vertebrates. By multitissue RT-PCR found Gpr149 highly expressed the ovary also brain digestive tract at low levels. levels are newborn ovaries but increase throughout folliculogenesis. In ovary, granulosa cells did not express...
Growth differentiation factor 9 (GDF9) is preferentially expressed in oocytes and essential for female fertility. To identify regulatory elements that confer high-level expression of GDF9 the ovary but repression other tissues, we generated transgenic mice which regions Gdf9 locus were fused to reporter genes. Two transgenes (-10.7/+5.6mGdf9-GFP) (-3.3/+5.6mGdf9-GFP) contained sequences either 10.7 or 3.3 kb upstream 5.6 downstream initiation codon demonstrated specifically oocytes, thereby...
Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells. We previously reported the identification of TEX14 as first essential intercellular bridge protein, demonstration required for male fertility, and finding utilize components cytokinesis machinery to form. Herein, we report RNA binding motif protein 44 (RBM44) a novel cell protein. RBM44 was identified by proteomic analysis after enrichment using marker is highly between mouse human contains...
Abstract Spermatogenesis is a tightly regulated process leading to the development of spermatozoa. To elucidate molecular spermatogenic mechanisms, we identified an acrosome‐specific gene AEP1 in spermatids, which located rat chromosome 17p14 with transcript size 3,091 bp encoding signal peptide, zinc finger‐like motif, coiled‐coil region, several predicted glycosylation and phosphorylation sites. Northern blot RT‐PCR analyses revealed restricted expression testis only. In postnatal testes,...
8113 Background: Cerebral metastases contribute most to the failure of locally advanced non-small cell lung cancer (NSCLC) management. Implications prophylactic cranial irradiation (PCI) for NSCLC are still controversial. Our study assessed risk factors brain in patients with postoperative NSCLC. PCI discussed. Methods: 233 treated surgical resection stage III-N2 were retrospective analyzed. To find out NSCLC, and establish mathematical model. Results: Median survival entire patient was 28...