Volatile organic compounds (VOCs) are directly associated with human health concerns and environmental safety. Therefore, it is urgent to achieve accurate detection of VOCs both qualitatively quantitatively. In this work, the qualitative ethyl benzene (EB), isopropyl alcohol (IPA), acetate (EA)—three pure in liquid phase—was discussed using terahertz (THz) microfluidic electromagnetic-induced transparency (EIT) meta-sensors. The THz response illustrated that an increase VOCs' volumes (1–...

For non-model plants, functional characterization of genes is still hampered by lack efficient stable transformation procedures. Here, we report a simple, fast and technique with Agrobacterium rhizogenes for generating transgenic roots in living plants to facilitate studies vivo. We showed that injection A. into stems various plant species lead root generation, which can sustain growth after the original, non-transgenic were cut off. A system was established pigeon pea, major woody food...

SUMMARY Calcineurin B‐like (CBL)‐interacting protein kinases (CIPKs) play a central role in Ca 2+ signalling and promote drought tolerance plants. The CIPK gene family pigeon pea ( Cajanus cajan L.), major food crop affected by drought, has not previously been characterised. Here, we identified 28 genes the genome. Five CcCIPK were strongly upregulated roots upon treatment selected for further characterisation. Overexpression of CcCIPK13 CcCIPK14 increased survival rates two‐ to three‐fold...

Congenital heart defects (CHD) is one of the most common birth in China. Many studies have examined risk factors for CHD, but their predictive abilities not been evaluated. In particular, few attempted to predict risks CHD from, necessarily unbalanced, population-based cross-sectional data. Therefore, we developed and validated machine learning models predicting, before during pregnancy, women's bearing children with CHD. We compared results these a large-scale, comprehensive retrospective...

Graphene has been widely used for various biological and biomedical applications due to its unique physiochemical properties. This study aimed evaluate the cardiotoxicity of graphene oxide (GO) reduced GO (rGO) in vitro vivo, as well investigate underlying toxicity mechanisms.GO was by gamma irradiation prepare rGO then characterized UV/visible light absorption spectroscopy. Rat myocardial cells (H9C2) were exposed or with different absorbed radiation doses. The cytotoxicity evaluated MTT...

Objective: Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway is closely related to tumorigenesis. Suppressors cytokine 3 (SOCS3) a negative regulator JAK-STAT pathway. MiR-340 expression significantly upregulated in gastric cancer (GC) tissue. This study investigated the role miR-340 regulating SOCS3 affecting GC cell proliferation, cycle, apoptosis.Patients methods: Dual luciferase assay was used verify targeted relationship between SOCS3. tissue...

Cancer cells use aerobic glycolysis to sustain their proliferation. Long non‑coding RNA brain cytoplasmic 1 (BCYRN1) has been reported act as an oncogene in non‑small‑cell lung cancer (NSCLC). The present study investigated the role of BCYRN1 NSCLC glycolysis. expression was detected and tissues using reverse transcription‑quantitative PCR. effect on examined by measuring cell glucose catabolism lactate synthesis. relationships between microRNA (miR)‑149, miR‑149 pyruvate kinase M1/2 (PKM2)...

Abstract Sorbitol is a major photosynthate produced in leaves and transported through the phloem of apple (Malus domestica) other tree fruits Rosaceae. stimulates its own metabolism, but underlying molecular mechanism remains unknown. Here, we show that sucrose nonfermenting 1 (SNF1)-related protein kinase (SnRK1) involved regulating sorbitol-responsive expression both SORBITOL DEHYDROGENASE (SDH1) ALDOSE-6-PHOSPHATE REDUCTASE (A6PR), encoding 2 key enzymes sorbitol metabolism. SnRK1...

Abstract Sorbitol is an important primary metabolite that serves as both a carbon source and signal to pathogens. The leaf diseases caused by Alternata alternata are particularly serious in crabapple ( Malus micromalus ). Here, we found sorbitol can enhance the resistance of A. R1 increasing content flavonoid catechin. Nanomaterials emerging technology tool efficiently deliver lncRNA target cells. nanoencapsulated lncRNA809 (SPc/lncRNA809) exhibits significant R1strain. To elucidate effect...

As the coronavirus disease-2019 (COVID-19) pandemic persists, post-COVID-19 syndrome (PS), characterized by symptoms like chest pain, fatigue, and palpitations, is becoming a significant medical social issue. COVID-19 patients with existing coronary artery disease (CAD) may face higher risks of complications. It crucial to assess if PS also have CAD, though data limited. We studied 75 68 non-COVID-19 admitted our hospital between 2022/12/20 2023/01/20. Demographic, laboratory, clinical were...

Hepatic encephalopathy (HE) is among the most common complications of cirrhosis. Data for cirrhosis with HE typically unbalanced. Traditional statistical methods and machine learning algorithms thus cannot identify a few classes. In this paper, we use to construct risk prediction model liver complicated by improve efficiency its prediction.We collected medical data from 1,256 patients performed preprocessing extract 81 features these irregular data. To predict in cirrhotic patients, compared...

Summary Aluminium (Al) toxicity decreases crop production in acid soils general, but many crops have evolved complex mechanisms to resist it. However, our current understanding of how plants cope with Al stress and perform resistance is still at the initial stage. In this study, citrate transporter CcMATE35 was identified be involved response. The release increased substantially over‐expression (OE) lines under stress, indicating enhanced resistance. It demonstrated that transcription factor...

BACKGROUND Ezrin-radixin-moesin (ERM) plays an important role in multiple links of tumors. It also involved breast cancer invasion and metastasis, might be a potential biomarker cancer. Another study suggested that ERM expression was regulated directly by miR-200c, had critical miR-200c suppressing cell migration. This aimed to investigate the effect miR-200b on line its influence metastasis ability vitro. MATERIAL AND METHODS Breast lines MCF-7 MDA-MB-231 with different metastatic...

Matrix metalloproteinase-9 (MMP-9) plays an important role in inflammation and matrix degradation involved atherosclerosis plaque rupture. The T allele of rs3918242 has been reported to lead a high promoter activity associate with the extent coronary artery disease (CAD). And some studies have that G rs17576 might be associated CAD. aim this study was assess association between polymorphisms MMP-9 gene CAD Chinese Han population.This case-control comprised 258 cases 153 controls from...

Abstract Flavonoids are secondary metabolites that play important roles in fruit and vegetable development. Here, we examined the function of hyperoside, a unique flavonoid okra (Abelmoschus esculentus), known to promote both flowering seed set. We showed exogenous application hyperoside significantly improved pollen germination rate tube growth by almost 50%, resulting 42.7% increase set rate. Of several genes induced treatment, AeUF3GaT1, which encodes an enzyme catalyzes last step...

Mediation analysis has been a useful tool for investigating the effect of mediators that lie in path from independent variable to outcome. With increasing dimensionality such as (epi)genomics studies, high-dimensional mediation model is needed. In this work, we focus on epigenetic studies with goal identify important DNA methylations act between an exposure disease Specifically, gene-based implemented kernel principal component capture potential nonlinear effect. We first review current...

The flowers of okra (Abelmoschus esculentus) open and wilt within only a few hours, this is accompanied by accumulation hyperoside, secondary metabolite in the flavonoid pathway. However, little known about relationship between flavonoids flowering. Here, we found that exogenous application hyperoside extended duration full-blooming period more than 3-fold, was 14.7-fold increase expression CALCIUM-DEPENDENT PROTEIN KINASE6 (AeCDPK6). Gene profiling indicated transcription factor AeMYB30...

As a multifunctional hormone-like molecule, melatonin exhibits pleiotropic role in plant salt stress tolerance. While actin cytoskeleton is essential to tolerance stress, it unclear if and how participates the melatonin-mediated alleviation of stress. Here, we report that alleviates damage pigeon pea by activating kinase-like protein, which interacts with an actin-depolymerizing factor. Cajanus cajan Actin-Depolymerizing Factor 9 (CcADF9) has function severing filaments highly expressed...

High-throughput omics data are generated almost with no limit nowadays. It becomes increasingly important to integrate different types disentangle the molecular machinery of complex diseases hope for better disease prevention and treatment. Since relationship among features typically unknown, a supervised learning model assuming particular distribution specific structure will not serve purpose capture underlying between multiple phenotype. In this work, we briefly reviewed methods kernel...

Recent studies have revealed the important role of long noncoding RNAs (lncRNAs) in heart development and pathogenesis. This study was aimed to investigate NEAT1 hypoxia-induced cardiac injury explore its possible molecular mechanism. Real-time PCR (RT-PCR) used determine relative RNA expression potential target microRNA, miR-129-5p, plasma patients with acute myocardial infarction, failure, angina, as well H2O2-treated H9c2 cells. The overexpression or inhibition cell migration...