A5103122107- Galectins and Cancer Biology
- Bacteriophages and microbial interactions
- Metal complexes synthesis and properties
- RNA modifications and cancer
- Signaling Pathways in Disease
- Phytochemicals and Antioxidant Activities
- Bacterial Genetics and Biotechnology
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Supramolecular Self-Assembly in Materials
- Macrophage Migration Inhibitory Factor
- Inorganic and Organometallic Chemistry
- Nanoparticle-Based Drug Delivery
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- Synthesis and biological activity
- Peroxisome Proliferator-Activated Receptors
- Microbial Metabolites in Food Biotechnology
- Drug Transport and Resistance Mechanisms
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Cholesterol and Lipid Metabolism
- Biochemical and Molecular Research
- Natural Products and Biological Research
Shaoguan University
2025
Tianjin University
2023
Northeast Normal University
2018-2020
University of Science and Technology of China
2017-2019
Hefei University
2019
High Magnetic Field Laboratory
2017
Zhejiang International Studies University
2007-2013
Jilin University
2003
The University of Texas Southwestern Medical Center
2003
Perilla frutescens (Perilla) has great potential for utilization in food and medicine due to the abundance of secondary metabolites, especially phenolic compounds. However, molecular mechanism underlying compound synthesis perilla remains poorly understood. By targeted metabolome analysis, we found nine differentially accumulated metabolites among QS2, QS6, QO7 leaves unique which only exist QS6. Furthermore, transcriptome analysis showed differential expression genes (DEGs) were...
The expression of prototype galectin‐14 (Gal‐14) in human placenta is higher than any other galectin, suggesting that it may play a role fetal development and regulation immune tolerance during pregnancy. Here, we solved the crystal structure dimeric Gal‐14 found its global fold significantly different from galectins with two β‐strands (S5 S6) extending one monomer contributing to carbohydrate‐binding domain other. hemagglutination assay showed this lectin could induce agglutination chicken...
In green species, sucrose could help them antagonize abiotic stress. Sucrose phosphate synthase (SPS) is a well-known rate limiting enzyme in the synthesis of sucrose. To date, however, no crystal structure SPS from species has been solved. this study, we report first co-crystal Thermosynechococcus elongatus with UDP and sucrose-6-phosphate (S6P). catalytic site, side chains His158 Glu331 together two groups make hydrogen bonds four hydroxyl glucose residue S6P, scenario that causes these to...
Charcot-Leyden crystal protein/Gal-10, abundantly expressed in eosinophils and basophils, is related to several immune diseases. Recently, crystallographic biochemical studies showed that Gal-10 cannot bind lactose, because a glutamate residue (Glu33) from another monomer blocks the binding site. Moreover, actually forms novel dimeric structure compared other galectins. To investigate role Glu33 plays inhibiting lactose binding, we mutated this glutamine, aspartate, alanine. The of E33A...
Galectin-13 (Gal-13) plays numerous roles in regulating the relationship between maternal and fetal tissues. Low expression levels or mutations of lectin can result pre-eclampsia. The previous crystal structure gel filtration data show that Gal-13 dimerizes via formation two disulfide bonds formed by Cys136 Cys138. In present study, we mutated them to serine (C136S, C138S C136S/C138S), crystalized variants solved their structures. All crystallized as monomers. C136S structure, Cys138 a bond...
Placental protein 13/galectin-13 (Gal-13) is highly expressed in placenta, where its lower expression related to pre-eclampsia. Recently, the crystal structures of wild-type Gal-13 and variant R53H at high resolution were solved. The crystallographic biochemical results showed that could not bind lactose. Here, we used site-directed mutagenesis re-engineer ligand binding site Gal-13, so it Of six newly engineered mutants, able solve four them. Three variants (R53HH57R, R53HH57RD33G...
In spite of their wide application, the cellular uptake platinum based anticancer drugs is still unclear. The copper transport protein, hCTR1, proposed to facilitate cisplatin, whereas organic cation (OCT) more important for oxaliplatin. It has been reported that both N-terminal and C-terminal metal binding motifs hCTR1 are highly reactive which initial step protein assisted cisplatin. unknown how in transfer cytoplasmic media, whether various complexes possess different activities this...
All living things have pyrophosphatases that hydrolyze pyrophosphate and release energy. This energetically favorable reaction drives many unfavorable reactions. An accepted catalytic model of pyrophosphatase shows a water molecule activated by two divalent cations (M1 M2) within the center can attack in an SN2 mechanism thus molecule. However, our co-crystal structure Acinetobacter baumannii with from solvent may, fact, be actual water. In wild-type pyrophosphate, electron density centers...
MDM2 is a well-known oncoprotein overexpressed in variety of cancers, and the identification inhibitors that disrupt MDM2/p53 interaction great interest anticancer drug development. Here we designed platform for facile visualizable using co-expressed protein complexes MDM2/p53. A hexahistidine-tag on allows binding complex to Ni-NTA affinity resin, while fluorescent fused p53 enables direct visualization with MDM2. Hence, inhibition can be observed naked eye. The assay set up by directly...
Abstract Background: Indomethacin is one of the non-steroidal anti-inflammatory drugs (NSAIDs) , and it was considered to have chemosensitization effects on many cancers, however, mechanism its effect chemotherapy still unclear. In this study, using human lung cancer SPC-A-1 cells, we examined indomethacin sensitize s cells cisplatin (DDP). Methods: Human were inoculated subcutaneously into BALB/c-nu/nu mice, all transplanted nude mice divided a control group, DDP group combined with after...