A5092614035- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- RNA Interference and Gene Delivery
EpiCypher (United States)
2023-2024
During tumor development, promoter CpG islands that are normally silenced by Polycomb repressive complexes (PRCs) become DNA-hypermethylated. The molecular mechanism which de novo DNA methyltransferase(s) [DNMT(s)] catalyze methylation at PRC-regulated regions remains unclear. Here, we report a cryo-electron microscopy structure of the DNMT3A long isoform (DNMT3A1) amino-terminal region in complex with nucleosome carrying PRC1-mediated histone H2A lysine-119 monoubiquitination (H2AK119Ub)....
Abstract Transcription activators are said to stimulate gene expression by “recruiting” coactivators promoters, yet this term fits several different kinetic models. To directly analyze dynamics of activator-coactivator interactions, single-molecule microscopy was used image promoter DNA, a transcription activator, and the Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex within nuclear extract. SAGA readily, but transiently, binds nucleosome-free DNA without while chromatin template association...