A5080691388- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Chemical Synthesis and Analysis
- Polydiacetylene-based materials and applications
- Thermodynamic properties of mixtures
- Surfactants and Colloidal Systems
- Supramolecular Self-Assembly in Materials
- Chemical and Physical Properties in Aqueous Solutions
- Mass Spectrometry Techniques and Applications
- Supramolecular Chemistry and Complexes
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Spectroscopy and Quantum Chemical Studies
- DNA and Nucleic Acid Chemistry
- Antibiotics Pharmacokinetics and Efficacy
- Crystallography and molecular interactions
- Genetics and Neurodevelopmental Disorders
- Advanced Polymer Synthesis and Characterization
- Pharmaceutical studies and practices
- RNA Research and Splicing
- Ionic liquids properties and applications
- Molecular Sensors and Ion Detection
- Synthesis and Characterization of Heterocyclic Compounds
- Protein Degradation and Inhibitors
Trinity University
2018-2024
EpiCypher (United States)
2023-2024
University of North Carolina at Chapel Hill
2021-2022
Baylor College of Medicine
2022
Harvard University
2013
Central Institute of Technology
1971
University of Bradford
1963-1970
Institute of Technology of Cambodia
1963
Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has been proposed that these PTMs form localized 'codes' are read by specialized regions (reader domains) chromatin-associated proteins (CAPs) to regulate downstream function. Substantial effort made define [CAP: histone PTM] specificities, and thus decipher the code guide epigenetic therapies. However, this largely done using reductive approach of isolated reader domains peptides, which cannot...
Protein-mimetic amphiphiles have significant promise as a platform to access the complex functions of natural biological materials and incorporate tunability environmental resilience synthetic materials. The fields polymer chemistry chemical biology concurrently approached development biomimetic with ranging from random amphiphilic copolymers peptide–lipid conjugates. In this Perspective, we strategies diverse arenas for controlling assembled morphologies dynamics protein-mimetic...
In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact N-termini availability the epigenetic machinery. Notably, H3 tail acetylation (e.g. K9ac, K14ac, K18ac) linked increased H3K4me3 engagement by BPTF PHD finger, but it unknown if this mechanism has a broader extension. Here, we show that promotes nucleosomal accessibility other H3K4 methyl readers, importantly, extends writers,...
Histone H2A lysine 119 (H2AK119Ub) is monoubiquitinated by Polycomb repressive complex 1 and deubiquitinated deubiquitinase (PR-DUB). PR-DUB cleaves H2AK119Ub to restrict focal at target sites protect active genes from aberrant silencing. The subunits (BAP1 ASXL1) are among the most frequently mutated epigenetic factors in human cancers. How establishes specificity for over other nucleosomal ubiquitination how disease-associated mutations of enzyme affect activity unclear. Here, we determine...
This Article describes the molecular recognition of peptides containing an N-terminal methionine (Met) by synthetic receptor cucurbit[8]uril (Q8) in aqueous solution and with submicromolar affinity. Prior work established that Q8 binds high affinity to aromatic amino acids, either simultaneous binding two residues, one from each different peptides, or residue its immediate neighbor on same peptide. The additional interface neighboring residues suggested possibility targeting nonaromatic...
During tumor development, promoter CpG islands that are normally silenced by Polycomb repressive complexes (PRCs) become DNA-hypermethylated. The molecular mechanism which de novo DNA methyltransferase(s) [DNMT(s)] catalyze methylation at PRC-regulated regions remains unclear. Here, we report a cryo-electron microscopy structure of the DNMT3A long isoform (DNMT3A1) amino-terminal region in complex with nucleosome carrying PRC1-mediated histone H2A lysine-119 monoubiquitination (H2AK119Ub)....
Chromatin is more than a simple genome packaging system, and instead locally distinguished by histone post-translational modifications (PTMs) that can directly change nucleosome structure / or be ″read″ chromatin-associated proteins to mediate downstream events. An accurate understanding of PTM binding preference vital explain normal function pathogenesis, has revealed multiple therapeutic opportunities. Such studies most often use peptides, even though these cannot represent the full...
Histone post-translational modifications (PTMs) often serve as distinct recognition sites for the recruitment of chromatin-associated proteins (CAPs) epigenome regulation. While CAP-PTM interactions have been extensively studied using histone peptides, this cannot consider regulatory potential multi-site binding on intact nucleosomes. To overcome limitation, we applied Nucleosome Mass Spectrometry (Nuc-MS), a native Top-Down MS approach that enables controlled disassembly CAP:nucleosome...
Journal Article The temperature dependence of the critical micelle concentrations cationic surface-active agents Get access J E Adderson, Adderson Postgraduate School Studies in Pharmacy, University Bradford, Yorks, UK Search for other works by this author on: Oxford Academic Google Scholar H Taylor Central Institute Technology, Petone, New Zealand Pharmacy and Pharmacology, Volume 23, Issue 4, April 1971, Pages 311–312, https://doi.org/10.1111/j.2042-7158.1971.tb08668.x Published: 12 2011...
Abstract The temperature dependence of the critical micelle concentrations decyl, dodecyl and tetradecyl α-picolinium bromides has been determined by measurement conductance. Constants for equation relating log. CMC alkyl chain length have calculated range 5° to 70°, in increments 5°. An estimate effect upon degree counterion binding is recorded. Thermodynamic parameters using uncharged phase-separation model a theoretical interpretation process micellization given.
ABSTRACT In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact N-termini availability the epigenetic machinery. Notably, H3 tail acetylation ( e.g. , K9ac, K14ac, K18ac) linked increased H3K4me3 engagement by BPTF PHD finger, but it unknown if this mechanism has broader extension. Here we show that promotes nucleosomal accessibility other H3K4 methyl readers, importantly, extends...
Abstract The maintenance of gene expression patterns during metazoan development is achieved by the actions Polycomb group (PcG) complexes. An essential modification marking silenced genes monoubiquitination histone H2A lysine 119 (H2AK119Ub) deposited E3 ubiquitin ligase activity non-canonical Repressive Complex 1. Deubiquitinase (PR-DUB) complex cleaves monoubiquitin from to restrict focal H2AK119Ub at target sites and protect active aberrant silencing. BAP1 ASXL1, subunits that form...
Peptide-polymer amphiphiles (PPAs) are tunable hybrid materials that achieve complex assembly landscapes by combining the sequence-dependent properties of peptides with structural diversity polymers. Despite their promise as biomimetic materials, determining how polymer and peptide simultaneously affect PPA self-assembly remains challenging. We herein present a systematic study structure-assembly relationships. PPAs containing oligo(ethyl acrylate) random-coil were used to determine role...
Abstract Twelve compounds derived from histamine have been prepared. In these the primary amine hydrogen atoms of are replaced by methyl groups and imidazole nucleus is substituted in two position a range aliphatic aromatic residues. Most members series histamine-like pharmacological properties; antihistamine activity.
During tumor development, promoter CpG islands (CGIs) that are normally silenced by Polycomb repressive complexes (PRCs) become DNA hypermethylated. The molecular mechanism which
Abstract Peptide–polymer amphiphiles (PPAs) are tunable hybrid materials that achieve complex assembly landscapes by combining the sequence‐dependent properties of peptides with structural diversity polymers. Despite their promise as biomimetic materials, determining how polymer and peptide simultaneously affect PPA self‐assembly remains challenging. We herein present a systematic study structure–assembly relationships. PPAs containing oligo(ethyl acrylate) random‐coil were used to determine...
Peptide polymer amphiphiles (PPAs) are highly tunable hybrid materials that achieve complex, protein-like assembly landscapes by combining sequence-dependent properties of peptides with structural diversity polymers. Despite their promise as functional biomimetic materials, determining how and peptide simultaneously affect PPA self-assembly remains challenging. We herein present a systematic study critical components within the design space dictate self-assembled morphologies. PPAs...