A5108750625- HIV Research and Treatment
- Genomics and Chromatin Dynamics
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Advanced biosensing and bioanalysis techniques
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Viral Infections and Vectors
- HIV, Drug Use, Sexual Risk
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Poxvirus research and outbreaks
- Acute Myeloid Leukemia Research
- Hepatitis Viruses Studies and Epidemiology
- RNA Research and Splicing
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Histone Deacetylase Inhibitors Research
- Virus-based gene therapy research
- Disaster Response and Management
- Neonatal Respiratory Health Research
- Viral Infections and Outbreaks Research
- Influenza Virus Research Studies
EpiCypher (United States)
2023-2025
National Institute of Allergy and Infectious Diseases
1997-2014
National Institutes of Health
2011-2014
Vanderbilt University
1991-1993
BackgroundEbolavirus and Marburgvirus cause severe hemorrhagic fever with high mortality are potential bioterrorism agents. There no available vaccines or therapeutic Previous clinical trials evaluated transmembrane-deleted point-mutation Ebolavirus glycoproteins (GPs) in candidate vaccines. Constructs this trial encode wild-type (WT) GP from Zaire Sudan species the Angola strain expressed a DNA vaccine.
Journal Article Augmentation of Human Immunodeficiency Virus Type 1 Neutralizing Antibody by Priming with gp160 Recombinant Vaccinia and Boosting in Vaccinia-Naive Adults Get access Barney S. Graham, Graham Reprints or correspondence: Dr. Dept. Medicine, Division Infectious Diseases, A-3310 MCN, Vanderbilt University School Nashville, TN 37232. Search for other works this author on: Oxford Academic PubMed Google Scholar Thomas J. Matthews, Matthews Robert B. Belshe, Belshe Mary Lou Clements,...
The safety and immunogenicity of a human immunodeficiency virus type 1 (HIV-1) gp160 recombinant vaccinia (HIVAC-1e) vaccine was evaluated in vaccinia-naive, healthy adults at low risk for acquiring HIV-1 infection. Volunteers (n = 36) were randomized to receive HIVAC-1e or control two dosages by bifurcated needle puncture 0 2 months; 12 6 recipients received either 10(6) 10(7) pfu/mL each inoculation. There no significant difference lesion size, level viral replication, systemic symptoms...
Journal Article Safety and Immunogenicity of a Fully Glycosylated Recombinant gp160 Human Immunodeficiency Virus Type 1 Vaccine in Subjects at Low Risk Infection Get access Robert B. Belshe, Belshe Reprints or correspondence: Dr. Division Infectious Diseases, Dept. Medicine, St. Louis University School 1402 S. Grand Blvd., Louis, MO 63104. Search for other works by this author on: Oxford Academic PubMed Google Scholar Mary Lou Clements, Clements Raphael Dolin, Dolin Barney Graham, Graham...
BALB/c mice were depleted of B cells by anti-mu treatment to investigate the pathogenesis respiratory syncytial virus (RSV) infection in absence antibody. Termination RSV replication after primary occurred with same kinetics anti-mu-treated as phosphate-buffered saline (PBS)-treated controls. Yet, when rechallenged, more permissive than PBS-treated Anti-mu-treated also experienced greater illness controls during both and rechallenge. Passive transfer RSV-specific immune serum before...
Histone post-translational modifications (PTMs) often serve as distinct recognition sites for the recruitment of chromatin-associated proteins (CAPs) epigenome regulation. While CAP-PTM interactions have been extensively studied using histone peptides, this cannot consider regulatory potential multi-site binding on intact nucleosomes. To overcome limitation, we applied Nucleosome Mass Spectrometry (Nuc-MS), a native Top-Down MS approach that enables controlled disassembly CAP:nucleosome...
The first generation of candidate vaccines to prevent HIV infection consisted recombinant envelope proteins (Env, gp120, and gp160) derived from a single laboratory strain HIV, designated IIIB/LAV, but produced with different expression systems. In this study we examined the fine specificity human Ab response each vaccine compared them responses workers infected same HIV. best responders protocol were studied compared. Detailed comparisons possible because all immunologic assays performed...
Abstract Transcription activators are said to stimulate gene expression by “recruiting” coactivators promoters, yet this term fits several different kinetic models. To directly analyze dynamics of activator-coactivator interactions, single-molecule microscopy was used image promoter DNA, a transcription activator, and the Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex within nuclear extract. SAGA readily, but transiently, binds nucleosome-free DNA without while chromatin template association...
In an unmodified state, positively charged histone N-terminal tails engage nucleosomal DNA in a manner which restricts access to not only the underlying DNA, but also key tail residues subject binding and/or modification. Charge-neutralizing modifications, such as acetylation, serve disrupt this DNA-tail interaction, facilitating residues. We previously showed that polyacetylation-mediated chromatin "switch" governs read-write capability of H3K4me3 by MLL1 methyltransferase complex. Here, we...