A5082386508- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Fibroblast Growth Factor Research
- Organ Transplantation Techniques and Outcomes
- Pediatric Hepatobiliary Diseases and Treatments
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Hepatitis Viruses Studies and Epidemiology
- Systemic Lupus Erythematosus Research
- Drug-Induced Hepatotoxicity and Protection
- Tryptophan and brain disorders
- Viral Infections and Vectors
- Diabetes and associated disorders
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- Retinoids in leukemia and cellular processes
- Advanced Breast Cancer Therapies
Northwest University
2025
Dazhou Central Hospital
2025
National Institute of Diabetes and Digestive and Kidney Diseases
2015-2024
National Institutes of Health
2013-2023
Shenzhen Maternity and Child Healthcare Hospital
2017-2020
Shenzhen University Health Science Center
2020
Putian University
2017
National Institute of Allergy and Infectious Diseases
2006-2015
National Center for Advancing Translational Sciences
2014
National Cancer Institute
1992-1997
Hepatitis C virus (HCV) infection is a major cause of end-stage liver disease and leading indication for transplantation. Current therapy fails in many instances associated with significant side effects. HCV encodes only few proteins depends heavily on host factors propagation. Each these dependencies potential therapeutic target. To find required by HCV, we completed genome-wide small interfering RNA (siRNA) screen using an infectious cell culture system. We applied two-part screening...
VRC-HIVMAB060-00-AB (VRC01) is a broadly neutralizing HIV-1 monoclonal antibody (mAb) isolated from the B cells of an HIV-infected patient. It directed against CD4 binding site and capable potently majority diverse strains. This Phase I dose-escalation study in healthy adults was conducted at National Institutes Health (NIH) Clinical Center (Bethesda, MD, USA). Primary objectives were safety, tolerability pharmacokinetics (PK) VRC01 intravenous (i.v.) infusion 5, 20 or 40 mg/kg, given either...
The hepatitis B virus X protein (HBX) is essential for the establishment of HBV infection in vivo and exerts a pleiotropic effect on diverse cellular functions. yeast two-hybrid system had indicated that HBX could interact with two subunits 26S proteasome. Here we demonstrate an association proteasome complex by coimmunoprecipitation colocalization upon sucrose gradient centrifugation. Expression HepG2 cells caused modest decrease proteasome's chymotrypsin- trypsin-like activities hydrolysis...
Recombinant hepatitis C virus (HCV)-like particles (HCV-LPs) containing HCV structural proteins (core, E1, and E2) produced in insect cells resemble the putative virions are capable of inducing strong broad humoral cellular immune responses mice baboons. Here, we present evidence on immunogenicity induction protective immunity by HCV-LPs chimpanzees. Chimpanzees (two each group), were immunized with or plus AS01B adjuvant. After immunizations, all animals developed an HCV-specific response...
The combination of pegylated interferon (PEG-IFN) and ribavirin is the standard treatment for chronic hepatitis C. Our recent clinical study suggests that augments induction interferon-stimulated genes (ISGs) in patients treated C virus (HCV) infection. In order to further characterize mechanisms action ribavirin, we examined effect on ISG cell culture. addition, infectious HCV culture systems was studied. Similar (IFN)-α, potently inhibits JFH-1 infection Huh7.5.1 cells a dose-dependent...
Hepatitis C virus (HCV) infection affects an estimated 185 million people worldwide, with chronic often leading to liver cirrhosis and hepatocellular carcinoma. Although HCV is curable, there unmet need for the development of effective affordable treatment options. Through a cell-based high-throughput screen, we identified chlorcyclizine HCl (CCZ), over-the-counter drug allergy symptoms, as potent inhibitor infection. CCZ inhibited in human hepatoma cells primary hepatocytes. The mode action...
BackgroundEbolavirus and Marburgvirus cause severe hemorrhagic fever with high mortality are potential bioterrorism agents. There no available vaccines or therapeutic Previous clinical trials evaluated transmembrane-deleted point-mutation Ebolavirus glycoproteins (GPs) in candidate vaccines. Constructs this trial encode wild-type (WT) GP from Zaire Sudan species the Angola strain expressed a DNA vaccine.
A previous study demonstrated decreased allergenicity in vitro of some food allergens after conjugation with polyphenols. However, little is known about how polyphenol affects vivo allergenicity. We conjugated a well-known allergen, ovalbumin (OVA), quercetin (QUE) to assess the potential OVA and BALB/c mouse model. QUE could covalently conjugate changed protein structure, which might destroy and/or mask epitopes. Conjugation IgE binding properties release capacity OVA. In vivo, as compared...
Abstract Since the emergence of Omicron variants at end 2021, they quickly became dominant globally. The may be more easily transmitted compared to earlier Wuhan and other variants. In this study, we aimed elucidate mechanisms altered infectivity associated with We systemically evaluated mutations located in S2 sequence spike identified that are responsible for viral fusion. demonstrated near S1/S2 cleavage site decrease cleavage, resulting reduced fusogenicity. Mutations HR1 sequences also...
Hepatitis B virus (HBV) has a unique fourth open reading frame coding for 16.5-kDa protein known as hepatitis X (HBX). The importance of HBX in the life cycle HBV been well established, but underlying molecular function remains controversial. We previously identified proteasome subunit PSMA7 that interacts specifically with Saccharomyces cerevisiae two-hybrid system. Here we demonstrate PSMC1, an ATPase-like 19 S component, also and PSMA7. Analysis interacting domains among PSMA7, by...
We have previously reported the production of hepatitis C virus-like particles (HCV-LP) using a recombinant baculovirus containing cDNA HCV structural proteins (core, E1, and E2). These resemble putative virions are capable inducing strong broad humoral cellular immune responses in mice. Here we present evidence on immunogenicity HCV-LP effects novel adjuvant systems nonhuman primate model, baboon. Three groups four baboons were immunized with HCV-LP, AS01B (monophosphoryl lipid A QS21), or...
Detection of RNAs on microarrays is rapidly becoming a standard approach for molecular biologists. However, current methods frequently discriminate against structured and/or small RNA species. Here we present an that bypasses these problems. Unmodified hybridized directly to DNA and detected with the high-affinity, nucleotide sequence-independent, DNA/RNA hybrid-specific mouse monoclonal antibody S9.6. Subsequent reactions fluorescently-labeled anti-mouse IgG or biotin-labeled together...
Recent functional genomics studies including genome-wide small interfering RNA (siRNA) screens demonstrated that hepatitis C virus (HCV) exploits an extensive network of host factors for productive infection and propagation. How these co-opted functions interact with various steps HCV replication cycle exert pro- or antiviral effects on remains largely undefined. Here we present unbiased systematic strategy to functionally interrogate dependencies uncovered from our previous infectious...
The establishment of protocols to differentiate human pluripotent stem cells (hPSCs) including embryonic (ESC) and induced (iPSC) into functional hepatocyte-like (HLCs) creates new opportunities study liver metabolism, genetic diseases infection hepatotropic viruses (hepatitis B C viruses) in the context specific background. While supporting efficient differentiation HLCs, published are limited terms fully mature hepatocytes a smaller-well format. This limitation handicaps application these...
NAFLD is a growing public health burden. However, the pathogenesis of has not yet been fully elucidated, and importance genetic factors only recently appreciated. Genomic studies have revealed strong association between progression I148M variant in patatin-like phospholipase domain-containing protein 3 (PNPLA3). Nonetheless, very little known about mechanisms by which this gene its variants can influence disease development. To investigate these mechanisms, we developed an vitro model that...
Both the level of expression and cellular distribution transcripts for transforming growth factor-alpha (TGF-alpha) were studied in adult rat liver after partial hepatectomy during hepatic differentiation fetal, neonatal, livers by northern blot analysis situ hybridization. A marked increase TGF-alpha was observed neonatal regeneration following modification Solt-Farber protocol. Quantitation silver grains hybridization with a riboprobe revealed sixfold to eightfold fetal hepatocytes....
Class B scavenger receptors (SR-Bs) bind lipoproteins and play an important role in lipid metabolism. Most recently, SR-B type I (SR-BI) its splicing variant SR-BII have been found to mediate bacterial adhesion cytosolic invasion mammalian cells. In this study, we demonstrate that SR-BI is a key host factor required for hepatitis C virus (HCV) uptake cross-presentation by human dendritic cells (DCs). Whereas monocytes T were characterized very low or undetectable expression levels, DCs...
Alcohol consumption and viral hepatitis infection synergistically accelerate liver injury, but the underlying mechanism is not fully understood. Here we have examined effects of ethanol on B protein X (HBX)- or C core (HCV protein)-mediated activation NF-kappaB, a critical signal in hepatic regeneration, tumor transformation. Acute acetaldehyde exposure potentiates HBX HCV NF-kappaB primary mouse hepatocytes. Such potentiation can be abolished by blocking metabolism overexpression dominant...