Nancy C.P. Leong
A5068549612- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Sphingolipid Metabolism and Signaling
- Neuroscience and Neuropharmacology Research
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Biomedical Research and Pathophysiology
- Lysosomal Storage Disorders Research
- Erythrocyte Function and Pathophysiology
- Cannabis and Cannabinoid Research
- Calcium signaling and nucleotide metabolism
- HIV Research and Treatment
- Folate and B Vitamins Research
- Diet and metabolism studies
National University of Singapore
2022-2025
Yong In University
2024
Accumulation of sphingolipids, especially sphingosines, in the lysosomes is a key driver several lysosomal storage diseases. The transport mechanism for sphingolipids from lysosome remains unclear. Here, we identified SPNS1, which shares highest homology to SPNS2 - sphingosine-1-phosphate (S1P) transporter, functions as transporter lysolipids lysosome. We generated Spns1 knockout cells and mice employed lipidomic metabolomic approaches reveal SPNS1 ligand identity. Global caused embryonic...
Abstract Human feline leukaemia virus subgroup C receptor-related proteins 1 and 2 (FLVCR1 FLVCR2) are members of the major facilitator superfamily . Their dysfunction is linked to several clinical disorders, including PCARP, HSAN Fowler syndrome 2–7 Earlier studies concluded that FLVCR1 may function as a haem exporter 8–12 , whereas FLVCR2 was suggested act importer 13 yet conclusive biochemical detailed molecular evidence remained elusive for both transporters 14–16 Here, we show...
Abstract Mutations in the orphan transporter MFSD7c (also known as Flvcr2 ), are linked to Fowler syndrome. Here, we used Mfsd7c knockout ( –/– ) mice and cell-based assays reveal that is a choline at blood–brain barrier (BBB). We performed comprehensive metabolomics analysis detected differential changes of metabolites brains livers embryos. Particularly, found choline-related were altered but not Thus, hypothesized regulates level brain. Indeed, expression human cells significantly...
Abstract FLVCR1 encodes Feline leukemia virus subgroup C receptor 1 (FLVCR1), a solute carrier (SLC) transporter within the Major Facilitator Superfamily. is widely expressed transmembrane protein with plasma membrane and mitochondrial isoforms implicated in heme, choline, ethanolamine transport. While Flvcr1 knockout mice die utero skeletal malformations defective erythropoiesis reminiscent of Diamond-Blackfan anemia, rare biallelic pathogenic variants are linked to childhood or adult-onset...
Abstract Human feline leukemia virus subgroup C receptor-related proteins 1 and 2 (FLVCR1 2) are members of the major facilitator superfamily . Their dysfunction is linked to several clinical disorders, including PCARP, HSAN, Fowler syndrome 2–7 Earlier studies concluded that FLVCR1 may function as a putative heme exporter 8–12 , while FLVCR2 was suggested act importer 13 yet conclusive biochemical detailed molecular evidence remained elusive for both transporters 14–17 Here, we show...
Abstract Mutations of MFSD7c (also known as Flvcr2 ), which is an orphan transporter, are linked to Fowler syndrome 1, 2 . Here, we use Mfsd7c knockout mice and cell-based assays reveal that a choline transporter at the blood-brain barrier (BBB). We performed comprehensive metabolomics detected differential changes metabolites in brains livers ( −/− ) embryos. Particularly, found choline-related were altered but not Thus, hypothesized regulates levels brain. Indeed, expression human cells...
Abstract Accumulation of sphingolipids, especially sphingosines, in the lysosomes is attributed to pathogenesis several lysosomal storage diseases. In search for a protein that mediates release we identified SPNS1 which shares highest homology SPNS2, sphingosine-1-phosphate (S1P) transporter. We generated knockout cells and mice Spns1 employed lipidomics metabolomics identify ligands. found knockouts resulted accumulation including sphingosines embryonic brains cell lines. These results...